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Literature summary for 1.2.1.65 extracted from

  • Zhao, H.; Li, Y.; Chen, W.; Cai, B.
    A novel salicylaldehyde dehydrogenase-NahV involved in catabolism of naphthalene from Pseudomonas putida ND6 (2007), Chin. Sci. Bull., 52, 1942-1948.
No PubMed abstract available
Search for more literature for 1.2.1.65

Application

Application Comment Organism
additional information two genes code for salicylaldehyde dehydrogenase. NahF resides in the naphthalene degradation upper pathway operon as the meta-cleavage pathway gene, whereas NahV is situated outside of this operon. NahF-like genes occur in all naphthalene-degradation bacteria, whereas nahV-like genes are present in only some naphthalene-degrading bacteria Pseudomonas putida
additional information two genes code for salicylaldehyde dehydrogenase. NahF resides in the naphthalene degradation upper pathway operon as the meta-cleavage pathway gene, whereas NahV is situated outside of this operon. NahF-like genes occurr in all naphthalene-degradation bacteria, whereas nahV-like genes are present in only some naphthalene-degrading bacteria Pseudomonas putida

Cloned(Commentary)

Cloned (Comment) Organism
nahF inserted into pET21b(+) and expressed in Escherichia coli BL21 (DE3) Pseudomonas putida
nahV inserted into pET21b(+) and expressed in Escherichia coli BL21 (DE3) Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
Al3+ inhibits NahF activity by 42%; inhibits NahV activity by 15% Pseudomonas putida
Ba2+ inhibits NahF activity by 62%; inhibits NahV activity by 52% Pseudomonas putida
Ca2+ inhibits NahF activity by 63%; inhibits NahV activity by 35% Pseudomonas putida
Cs+ inhibits NahF activity by 44%; inhibits NahV activity by 69% Pseudomonas putida
Cu2+ inhibits NahF activity by 70% Pseudomonas putida
Li+ inhibits NahF activity by 50%; inhibits NahV activity by 57% Pseudomonas putida
Mg2+ inhibits NahF activity by 34%; inhibits NahV activity by 36% Pseudomonas putida
Ni2+ inhibits NahF activity by 60%; inhibits NahV activity by 70% Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00016
-
 NAD+
-
 Pseudomonas putida
0.00109
-
 NAD+
-
 Pseudomonas putida
0.034
-
 Salicylaldehyde
-
 Pseudomonas putida
0.146
-
 Salicylaldehyde
-
 Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ enhances NahV activity by 63% activity Pseudomonas putida
Fe2+ enhances NahF activity by 40% activity Pseudomonas putida
Fe2+ enhances NahV activity by 152% Pseudomonas putida
additional information Zn2+ has no effect on NahF activity Pseudomonas putida
Zn2+ enhances NahV activity by 14% activity Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 1 * 50000, SDS-PAGE Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida Q6XUJ4
-
-
Pseudomonas putida Q6XUN9
-
-
Pseudomonas putida ND6 Q6XUJ4
-
-
Pseudomonas putida ND6 Q6XUN9
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tagged NahF purified by Ni-NTA chromatography Pseudomonas putida
His-tagged NahV purified by Ni-NTA chromatography Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-chlorosalicylaldehyde + NAD+ + H2O
-
 Pseudomonas putida 5-chlorosalicylate + NADH + H+
-
 ?
5-chlorosalicylaldehyde + NAD+ + H2O
-
 Pseudomonas putida ND6 5-chlorosalicylate + NADH + H+
-
 ?
caprylic aldehyde + NAD+ + H2O
-
 Pseudomonas putida caprylic acid + NADH + H+
-
 ?
caprylic aldehyde + NAD+ + H2O
-
 Pseudomonas putida ND6 caprylic acid + NADH + H+
-
 ?
formaldehyde + NAD+ + H2O
-
 Pseudomonas putida formate + NADH + H+
-
 ?
formaldehyde + NAD+ + H2O
-
 Pseudomonas putida ND6 formate + NADH + H+
-
 ?
glutaraldehyde + NAD+ + H2O
-
 Pseudomonas putida glutarate + NADH + H+
-
 ?
glutaraldehyde + NAD+ + H2O
-
 Pseudomonas putida ND6 glutarate + NADH + H+
-
 ?
glyoxal + NAD+ + H2O
-
 Pseudomonas putida glyoxylate + NADH + H+
-
 ?
m-nitrobenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida m-nitrobenzoate + NADH + H+
-
 ?
o-methoxybenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida o-methoxybenzoate + NADH + H+
-
 ?
o-methoxybenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida ND6 o-methoxybenzoate + NADH + H+
-
 ?
o-nitrobenzaldehyde + NAD+ + H2O
-
 Pseudomonas putida o-nitrobenzoate + NADH + H+
-
 ?
salicylaldehyde + NAD+ + H2O best substrate Pseudomonas putida salicylate + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 50000, SDS-PAGE Pseudomonas putida

Synonyms

Synonyms Comment Organism
NahF
-
 Pseudomonas putida
NahV
-
 Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 72.8% activity is retained for NahF after a 30 min exposure Pseudomonas putida
50
-
 NahV looses 88.8% activity after a 30 min exposure Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas putida

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas putida
-
-
 5.08
Pseudomonas putida
-
-
 5.63