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Literature summary for 1.2.1.65 extracted from
- Expression, purification and preliminary crystallographic studies of NahF, a salicylaldehyde dehydrogenase from Pseudomonas putida G7 involved in naphthalene degradation (2012), Acta Crystallogr. Sect. F, 68, 93-97.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| nahF gene subcloned into the pET28a(TEV) vector and the recombinant protein with 6xHis tag at the N-terminaus overexpressed in Escherichia coli BL21 Arctic Express (DE3) cells | Pseudomonas putida |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystals of recombinant 6xHis-NahF obtained at 291 K and diffracted to 2.42 A resolution | Pseudomonas putida |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 125000 | - |
- |
Pseudomonas putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
| Pseudomonas putida G7 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by Ni2+ affinity chromatography followed by gel filtration | Pseudomonas putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | nah operon in Pseudomonas putida, which is present on the 83 kb metabolic plasmid NAH7 includes nahF gene coding for salicylaldehyde dehydrogenase | Pseudomonas putida | ? | - |
? |  |
| additional information | nah operon in Pseudomonas putida, which is present on the 83 kb metabolic plasmid NAH7 includes nahF gene coding for salicylaldehyde dehydrogenase | Pseudomonas putida G7 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | suggested by the elution profile in the SEC chromatogram | Pseudomonas putida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NahF | - |
Pseudomonas putida |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | NahF catalyzes the last reaction in the conversion of naphthalene to salicylate | Pseudomonas putida |
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