Activating Compound | Comment | Organism | Structure |
---|---|---|---|
K+ | 125 mM KCl, 1.5 fold increase in activity | Acinetobacter baylyi | |
Na+ | 125 mM NaCl, 1.5 fold increase in activity | Acinetobacter baylyi |
Cloned (Comment) | Organism |
---|---|
into the vector pET-Duet1 for expression in Escherichia coli BL21DE3 cells | Acinetobacter baylyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
4-hydroxybenzaldehyde | apparent KM | Acinetobacter baylyi | |
0.029 | - |
benzaldehyde | apparent KM | Acinetobacter baylyi | |
0.037 | - |
3,4-Dihydroxybenzaldehyde | apparent KM | Acinetobacter baylyi | |
0.044 | - |
4-hydroxy-3-methoxybenzaldehyde | apparent KM | Acinetobacter baylyi | |
0.6 | - |
NAD+ | apparent KM | Acinetobacter baylyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
determined by SDS-PAGE | Acinetobacter baylyi |
116000 | - |
determined by gel filtration | Acinetobacter baylyi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter baylyi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
on a His-Trap FF IMAC column | Acinetobacter baylyi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.2 | - |
clarified lysate, total activity 37 U, protein concentration 5.77 mg/ml | Acinetobacter baylyi |
22 | - |
IMAC eluted 4-hydroxy benzaldehyde dehydrogenase, total activity 35 Units, protein concentration 0.27 mg/ml | Acinetobacter baylyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dihydroxybenzaldehyde + NAD+ + H2O | - |
Acinetobacter baylyi | 2,4-dihydroxybenzoate + NADH + H+ | - |
? | |
3,4-dihydroxybenzaldehyde + NAD+ + H2O | - |
Acinetobacter baylyi | 3,4-dihydroxybenzoate + NADH + H+ | - |
? | |
3-hydroxy-4-methoxybenzaldehyde + NAD+ + H2O | - |
Acinetobacter baylyi | 3-hydroxy-4-methoxybenzoate + NADH + H+ | - |
? | |
3-hydroxybenzaldehyde + NAD+ + H2O | - |
Acinetobacter baylyi | 3-hydroxybenzoate + NADH + H+ | - |
? | |
3-methoxybenzaldehyde + NAD+ + H2O | - |
Acinetobacter baylyi | 3-methoxybenzoate + NADH + H+ | - |
? | |
4-hydroxy-3-methoxybenzaldehyde + NAD+ + H2O | - |
Acinetobacter baylyi | 4-hydroxy-3-methoxybenzoate + NADH + H+ | - |
? | |
4-hydroxybenzaldehyde + NAD+ + H2O | - |
Acinetobacter baylyi | 4-hydroxybenzoate + NADH + H+ | - |
? | |
benzaldehyde + NAD+ + H2O | - |
Acinetobacter baylyi | benzoate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 55000 | Acinetobacter baylyi |
Synonyms | Comment | Organism |
---|---|---|
4-hydroxy benzaldehyde dehydrogenase | - |
Acinetobacter baylyi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
21 | - |
activity assay | Acinetobacter baylyi |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
21 | 45 | 21°C 100%, 37°C 90%, 45°C 75% activity | Acinetobacter baylyi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
4-hydroxybenzaldehyde | apparent Kcat | Acinetobacter baylyi | |
4.6 | - |
benzaldehyde | apparent Kcat | Acinetobacter baylyi | |
14 | - |
NAD+ | apparent Kcat | Acinetobacter baylyi | |
18 | - |
4-hydroxy-3-methoxybenzaldehyde | apparent Kcat | Acinetobacter baylyi | |
25 | - |
3,4-Dihydroxybenzaldehyde | apparent Kcat | Acinetobacter baylyi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.3 | - |
- |
Acinetobacter baylyi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.9 | 10.7 | the activities measured at pH 6.85 and 10.7 are approximately 30% of the activity measured at pH 9.3 | Acinetobacter baylyi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Acinetobacter baylyi |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Acinetobacter baylyi | determined by isoelectric focusing electrophoresis | - |
5.6 |
Acinetobacter baylyi | predicted from the amino acid sequence | - |
5.8 |