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Literature summary for 1.2.1.70 extracted from
- Physical and kinetic interactions between glutamyl-tRNA reductase and glutamate-1-semialdehyde aminotransferase of Chlamydomonas reinhardtii (2005), J. Biol. Chem., 280, 24301-24307.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| glutamate-1-semialdehyde aminotransferase | 2.5fold activity in presence of glutamate-1-semialdehyde aminotransferase | Chlamydomonas reinhardtii |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Chlamydomonas reinhardtii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C48A | no activity | Chlamydomonas reinhardtii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
2 * 52500 + 2 * 46000, sucrose gradient sedimentation of glutamyl-tRNA reductase preincubated with glutamate-1-semialdehyde aminotransferase. Both enzyme also co-precipitate in immunoprecipitation experiments | Chlamydomonas reinhardtii |
| 52500 | - |
2 * 52500 + 2 * 46000, sucrose gradient sedimentation of glutamyl-tRNA reductase preincubated with glutamate-1-semialdehyde aminotransferase. Both enzyme also co-precipitate in immunoprecipitation experiments | Chlamydomonas reinhardtii |
| 52500 | - |
2 * 52500, sucrose gradient sedimentation compared to calculation from sequence | Chlamydomonas reinhardtii |
| 101000 | - |
sucrose density gradient sedimentation, dimer | Chlamydomonas reinhardtii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlamydomonas reinhardtii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-affinity chromatography | Chlamydomonas reinhardtii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Chlamydomonas reinhardtii | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamyl-tRNAGlu + NADH + H+ | - |
Chlamydomonas reinhardtii | L-glutamate 1-semialdehyde + NAD+ + tRNAGlu | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 52500, sucrose gradient sedimentation compared to calculation from sequence | Chlamydomonas reinhardtii |
| heterotetramer | 2 * 52500 + 2 * 46000, sucrose gradient sedimentation of glutamyl-tRNA reductase preincubated with glutamate-1-semialdehyde aminotransferase. Both enzyme also co-precipitate in immunoprecipitation experiments | Chlamydomonas reinhardtii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glutamyl-tRNA reductase | - |
Chlamydomonas reinhardtii |
| GTR | - |
Chlamydomonas reinhardtii |
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Release 2023.1 (January 2023)
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