Cloned (Comment) | Organism |
---|---|
wild-type GluTR and variants produced as N-terminal His6-fusion proteins | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C50S | inactive | Escherichia coli |
E54K | retains 6% reductase and 2% esterase activity | Escherichia coli |
H99N | retains 5% reductase and 4% esterase activity | Escherichia coli |
Q116L | lacks reductase activity whereas 30% esterase activity is retained | Escherichia coli |
R52K | retains 5% reductase and 4% esterase activity | Escherichia coli |
R52Q | inactive | Escherichia coli |
S109A | retains 28% reductase and 30% esterase activity | Escherichia coli |
T49V | retains 10% reductase and 5% esterase activity | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A6X1 | - |
- |
Purification (Comment) | Organism |
---|---|
wild-type GluTR and variants | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutaminyl-tRNAGlu + NADPH + H+ | - |
Escherichia coli | ? + NADP+ + tRNAGlu | - |
? | |
L-glutamyl-tRNAGlu + NADPH + H+ | - |
Escherichia coli | L-glutamate 1-semialdehyde + NADP+ + tRNAGlu | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GluTR | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Escherichia coli |