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Literature summary for 1.2.1.70 extracted from
- Cellular levels of heme affect the activity of dimeric glutamyl-tRNA reductase (2011), Biochem. Biophys. Res. Commun., 405, 134-139.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| N-terminal His6-tagged GluTR expressed in Escherichia coli | Acidithiobacillus ferrooxidans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
SDSPAGE of His6-tagged GluTR | Acidithiobacillus ferrooxidans |
| 50490 | - |
MALDI-TOF mass spectrometry of His6-tagged GluTR | Acidithiobacillus ferrooxidans |
| 104000 | - |
cross-linking of GluTR with glutaraldehyde | Acidithiobacillus ferrooxidans |
| 148000 | - |
relative molecular mass of native recombinant GluTR after removal of His6-tag, determined by gel permeation chromatography | Acidithiobacillus ferrooxidans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acidithiobacillus ferrooxidans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by Ni2+-Sepharose affinity chromatography to near homogeneity | Acidithiobacillus ferrooxidans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamyl-tRNAGlu + NADPH + H+ | - |
Acidithiobacillus ferrooxidans | L-glutamate 1-semialdehyde + NADP+ + tRNAGlu | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 50000, probably enzyme has an overall V shape that makes the protein migrate slower in gel permeation chromatography | Acidithiobacillus ferrooxidans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GluTR | - |
Acidithiobacillus ferrooxidans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | enzyme shows Soret peak at 420 nm and a broad absorbance around 540 nm, data suggest that heme is bound preferentially to the dimeric form of GluTR | Acidithiobacillus ferrooxidans | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | GluTR is the first enzyme committed to tetrapyrrole biosynthesis by the C5-pathway | Acidithiobacillus ferrooxidans |
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Release 2023.1 (January 2023)
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