Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.1.70 extracted from

  • Kars, G.; Alparslan, U.e.
    Heterologous expression of glutamyl-tRNA reductase gene in Rhodobacter sphaeroides O.U.001 to enhance 5-aminolevulinic acid production (2014), Biotechnol. Biotechnol. Equip., 28, 1034-1041 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.70

Cloned(Commentary)

Cloned (Comment) Organism
recombinant enzyme expression in Rhodobacter sphaeroides strain DSM 5864, which synthesizes 5-aminolevulinic acid through the C-4 pathway and also has the genes coding for the enzymes of the C-5 pathway, except for glutamyl-tRNA reductase. Expression of the glutamyl-tRNA reductase gene from Rhodospirillum rubrum enabled the C-5 pathway in Rhodobacter sphaeroides to function upon assembling all the required genes Rhodospirillum rubrum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu Rhodospirillum rubrum
-
 L-glutamyl-tRNAGlu + NADPH + H+
-
 ?
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu Rhodospirillum rubrum DSM 467
-
 L-glutamyl-tRNAGlu + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodospirillum rubrum
-
-
-
Rhodospirillum rubrum DSM 467
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
 Rhodospirillum rubrum L-glutamyl-tRNAGlu + NADPH + H+
-
 ?
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
 Rhodospirillum rubrum DSM 467 L-glutamyl-tRNAGlu + NADPH + H+
-
 ?

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Rhodospirillum rubrum

General Information

General Information Comment Organism
physiological function the enzyme is required for the biosynthesis of 5-aminolevulinic acid Rhodospirillum rubrum