Literature summary for 1.2.1.72 extracted from

Naula, C.; Alibu, V.P.; Brock, J.M.; Veitch, N.J.; Burchmore, R.J.; Barrett, M.P.
A new erythrose 4-phosphate dehydrogenase coupled assay for transketolase (2008), J. Biochem. Biophys. Methods, 70, 1185-1187.

Search for more literature for 1.2.1.72

Application

Application	Comment	Organism
degradation	coupling of a transketolase reaction (using Leishmania mexicana transketolase) that converts D-fructose 6-phosphate to D-erythrose 4-phosphate, which can then be converted to 4-phosphate D-erythronate using E4PD, whereby D-ribose 5-phosphate and D-glyceraldehyde 3-phosphate can both be used as ketol acceptor substrates in the reaction	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0A9B6	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-erythrose 4-phosphate + NAD+	production of E4P can be coupled to the NADH generating E4PD activity which enables measurement of the transketolase reaction involving F6P as donor substrate	Escherichia coli	4-phosphoerythronate + NADH	-	?
D-ribose 5-phosphate + NAD+	D-Ribose 5-phosphate can be used as ketol acceptor substrate in the reaction although D-ribose 5-phosphate is also a substrate for the coupling enzyme	Escherichia coli	? + NADH	-	?
additional information	fructose 6-phosphate, glycerol 6-phosphate and glyceraldehyde 3-phosphate can not be used as substrate by E4PD when added up to 50 mM	Escherichia coli	?	-	?

Synonyms

Synonyms	Comment	Organism
E4PD	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Escherichia coli

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)