Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 2fold stimulation at 5 mM | Sulfurisphaera tokodaii |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sulfurisphaera tokodaii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | - |
Sulfurisphaera tokodaii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
NADPH | pH 7.8, 65°C | Sulfurisphaera tokodaii | |
0.04 | - |
malonyl-CoA | pH 7.8, 65°C | Sulfurisphaera tokodaii | |
0.1 | - |
malonyl-CoA | pH 7.8, 65°C | Metallosphaera sedula |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 2fold stimulation at 5 mM | Sulfurisphaera tokodaii | |
Mn2+ | 2fold stimulation at 5 mM | Sulfurisphaera tokodaii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
or dimer, 4 * 39000, calculated, 4 * 45000, SDS-PAGE, enzyme contains bound RNA | Sulfurisphaera tokodaii |
39000 | - |
or tetramer, 2 * 39000, calculated, 2 * 45000, SDS-PAGE, enzyme contains bound RNA | Sulfurisphaera tokodaii |
43000 | - |
4 * 43000, SDS-PAGE | Metallosphaera sedula |
45000 | - |
or dimer, 4 * 39000, calculated, 4 * 45000, SDS-PAGE, enzyme contains bound RNA | Sulfurisphaera tokodaii |
45000 | - |
or tetramer, 2 * 39000, calculated, 2 * 45000, SDS-PAGE, enzyme contains bound RNA | Sulfurisphaera tokodaii |
150000 | - |
gel filtration | Metallosphaera sedula |
160000 | - |
gel filtration | Sulfurisphaera tokodaii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Metallosphaera sedula | - |
- |
- |
Sulfurisphaera tokodaii | Q96YK1 | - |
- |
Purification (Comment) | Organism |
---|---|
partly | Metallosphaera sedula |
recombinant protein | Sulfurisphaera tokodaii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.6 | - |
pH 7.8, 65°C | Metallosphaera sedula |
44 | - |
pH 7.8, 65°C | Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonate semialdehyde + coenzyme A + NADP+ | - |
Sulfurisphaera tokodaii | malonyl-CoA + NADPH + H+ | - |
r | |
malonyl-CoA + NADPH + H+ | - |
Metallosphaera sedula | malonate semialdehyde + coenzyme A + NADP+ | - |
? | |
malonyl-CoA + NADPH + H+ | - |
Sulfurisphaera tokodaii | malonate semialdehyde + coenzyme A + NADP+ | - |
r | |
succinate semialdehyde + coenzyme A + NADP+ | - |
Sulfurisphaera tokodaii | succinyl-CoA + NADPH + H+ | - |
r | |
succinyl-CoA + NADPH + H+ | at 25% of the rate with malonyl-CoA | Sulfurisphaera tokodaii | succinate semialdehyde + coenzyme A + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | or tetramer, 2 * 39000, calculated, 2 * 45000, SDS-PAGE, enzyme contains bound RNA | Sulfurisphaera tokodaii |
More | enzyme in its native state is associated with small RNA | Sulfurisphaera tokodaii |
tetramer | 4 * 43000, SDS-PAGE | Metallosphaera sedula |
tetramer | or dimer, 4 * 39000, calculated, 4 * 45000, SDS-PAGE, enzyme contains bound RNA | Sulfurisphaera tokodaii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 8 | malonyl-CoA | per subunit, pH 7.8, 65°C | Sulfurisphaera tokodaii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Sulfurisphaera tokodaii |
7.8 | - |
- |
Metallosphaera sedula |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | - |
half-maximal activity | Sulfurisphaera tokodaii |
6.5 | - |
half-maximal activity | Metallosphaera sedula |
8 | - |
half-maximal activity | Sulfurisphaera tokodaii |
8.5 | - |
half-maximal activity | Metallosphaera sedula |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
coenzyme A | - |
Sulfurisphaera tokodaii | |
additional information | no cofactor: NADH | Metallosphaera sedula | |
additional information | no cofactor: NADH, phosphate | Sulfurisphaera tokodaii | |
NADP+ | - |
Sulfurisphaera tokodaii | |
NADPH | - |
Metallosphaera sedula | |
NADPH | - |
Sulfurisphaera tokodaii |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.04 | - |
pH 7.8, 65°C | Sulfurisphaera tokodaii | iodoacetamide |
Organism | Comment | Expression |
---|---|---|
Metallosphaera sedula | in heterotropically grown cells, activity is downregulated to 40 nmol per min and mg | down |
Metallosphaera sedula | under autotrophic conditions, 10fold upregulation | up |