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Literature summary for 1.2.1.75 extracted from

  • Alber, B.; Olinger, M.; Rieder, A.; Kockelkorn, D.; Jobst, B.; Hägler, M.; Fuchs, G.
    Malonyl-coenzyme A reductase in the modified 3-hydroxypropionate cycle for autotrophic carbon fixation in archaeal Metallosphaera and Sulfolobus spp (2006), J. Bacteriol., 188, 8551-8559.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 2fold stimulation at 5 mM Sulfurisphaera tokodaii

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sulfurisphaera tokodaii

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide
-
Sulfurisphaera tokodaii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
NADPH pH 7.8, 65°C Sulfurisphaera tokodaii
0.04
-
malonyl-CoA pH 7.8, 65°C Sulfurisphaera tokodaii
0.1
-
malonyl-CoA pH 7.8, 65°C Metallosphaera sedula

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 2fold stimulation at 5 mM Sulfurisphaera tokodaii
Mn2+ 2fold stimulation at 5 mM Sulfurisphaera tokodaii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
or dimer, 4 * 39000, calculated, 4 * 45000, SDS-PAGE, enzyme contains bound RNA Sulfurisphaera tokodaii
39000
-
or tetramer, 2 * 39000, calculated, 2 * 45000, SDS-PAGE, enzyme contains bound RNA Sulfurisphaera tokodaii
43000
-
4 * 43000, SDS-PAGE Metallosphaera sedula
45000
-
or dimer, 4 * 39000, calculated, 4 * 45000, SDS-PAGE, enzyme contains bound RNA Sulfurisphaera tokodaii
45000
-
or tetramer, 2 * 39000, calculated, 2 * 45000, SDS-PAGE, enzyme contains bound RNA Sulfurisphaera tokodaii
150000
-
gel filtration Metallosphaera sedula
160000
-
gel filtration Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Metallosphaera sedula
-
-
-
Sulfurisphaera tokodaii Q96YK1
-
-

Purification (Commentary)

Purification (Comment) Organism
partly Metallosphaera sedula
recombinant protein Sulfurisphaera tokodaii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6
-
pH 7.8, 65°C Metallosphaera sedula
44
-
pH 7.8, 65°C Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonate semialdehyde + coenzyme A + NADP+
-
Sulfurisphaera tokodaii malonyl-CoA + NADPH + H+
-
r
malonyl-CoA + NADPH + H+
-
Metallosphaera sedula malonate semialdehyde + coenzyme A + NADP+
-
?
malonyl-CoA + NADPH + H+
-
Sulfurisphaera tokodaii malonate semialdehyde + coenzyme A + NADP+
-
r
succinate semialdehyde + coenzyme A + NADP+
-
Sulfurisphaera tokodaii succinyl-CoA + NADPH + H+
-
r
succinyl-CoA + NADPH + H+ at 25% of the rate with malonyl-CoA Sulfurisphaera tokodaii succinate semialdehyde + coenzyme A + NADP+
-
r

Subunits

Subunits Comment Organism
dimer or tetramer, 2 * 39000, calculated, 2 * 45000, SDS-PAGE, enzyme contains bound RNA Sulfurisphaera tokodaii
More enzyme in its native state is associated with small RNA Sulfurisphaera tokodaii
tetramer 4 * 43000, SDS-PAGE Metallosphaera sedula
tetramer or dimer, 4 * 39000, calculated, 4 * 45000, SDS-PAGE, enzyme contains bound RNA Sulfurisphaera tokodaii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 8 malonyl-CoA per subunit, pH 7.8, 65°C Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
Sulfurisphaera tokodaii
7.8
-
-
Metallosphaera sedula

pH Range

pH Minimum pH Maximum Comment Organism
6
-
half-maximal activity Sulfurisphaera tokodaii
6.5
-
half-maximal activity Metallosphaera sedula
8
-
half-maximal activity Sulfurisphaera tokodaii
8.5
-
half-maximal activity Metallosphaera sedula

Cofactor

Cofactor Comment Organism Structure
coenzyme A
-
Sulfurisphaera tokodaii
additional information no cofactor: NADH Metallosphaera sedula
additional information no cofactor: NADH, phosphate Sulfurisphaera tokodaii
NADP+
-
Sulfurisphaera tokodaii
NADPH
-
Metallosphaera sedula
NADPH
-
Sulfurisphaera tokodaii

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.04
-
pH 7.8, 65°C Sulfurisphaera tokodaii iodoacetamide

Expression

Organism Comment Expression
Metallosphaera sedula in heterotropically grown cells, activity is downregulated to 40 nmol per min and mg down
Metallosphaera sedula under autotrophic conditions, 10fold upregulation up