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Literature summary for 1.2.1.78 extracted from

  • Iwabuchi, T.; Harayama, S.
    Biochemical and genetic characterization of 2-carboxybenzaldehyde dehydrogenase, an enzyme involved in phenanthrene degradation by Nocardioides sp. strain KP7 (1997), J. Bacteriol., 179, 6488-6494.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.78

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Nocardioides sp.

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 10 mM, about 30% inhibition Nocardioides sp.
EDTA 10 mM, 28% inhibition Nocardioides sp.
ethylenedioxybis(ethylamine)-N,N,N',N'-tetraacetic acid 10 mM, 14% inhibition Nocardioides sp.
Mg2+ 10 mM, about 30% inhibition Nocardioides sp.
Mn2+ 10 mM, about 30% inhibition Nocardioides sp.
additional information less than 15% inhibition in presence of 10 mM Zn2+, Co2+ or Cu2+ Nocardioides sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.083
-
 NAD+ pH 9.4, 25°C Nocardioides sp.
0.1
-
 2-carboxybenzaldehyde pH 9.4, 25°C Nocardioides sp.
2.8
-
 2-carboxybenzaldehyde pH 9.4, 25°C Nocardioides sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 4 * 53000, SDS-PAGE Nocardioides sp.
205000
-
 gel filtration Nocardioides sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-carboxybenzaldehyde + NAD+ + H2O Nocardioides sp. the enzyme is involved in phenanthrene degradation o-phthalic acid + NADH + H+
-
 ?
2-carboxybenzaldehyde + NAD+ + H2O Nocardioides sp. KP7 the enzyme is involved in phenanthrene degradation o-phthalic acid + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Nocardioides sp. Q79EM7
-
-
Nocardioides sp. KP7 Q79EM7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Nocardioides sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:phenanthrene-grown cell
-
 Nocardioides sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-carboxybenzaldehyde + NAD+ + H2O
-
 Nocardioides sp. o-phthalic acid + NADH + H+
-
 ?
2-carboxybenzaldehyde + NAD+ + H2O the enzyme is involved in phenanthrene degradation Nocardioides sp. o-phthalic acid + NADH + H+
-
 ?
2-carboxybenzaldehyde + NAD+ + H2O
-
 Nocardioides sp. KP7 o-phthalic acid + NADH + H+
-
 ?
2-carboxybenzaldehyde + NAD+ + H2O the enzyme is involved in phenanthrene degradation Nocardioides sp. KP7 o-phthalic acid + NADH + H+
-
 ?
2-nitrobenzaldehyde + NAD+ + H2O
-
 Nocardioides sp. ?
-
 ?
2-nitrobenzaldehyde + NAD+ + H2O
-
 Nocardioides sp. KP7 ?
-
 ?
additional information no activity with: 3-carboxybenzaldehyde, 4-carboxybenzaldehyde, benzaldehyde, salicylaldehyde, 2-methylbenzaldehyde, 2-chlorobenzaldehyde, 2-fluorobenzaldehyde, 2-benzaldehydesulfonate, 2-methoxybenzaldehyde, 1-hydroxy-2-naphthoaldehyde and n-hexylaldehyde Nocardioides sp. ?
-
 ?
additional information no activity with: 3-carboxybenzaldehyde, 4-carboxybenzaldehyde, benzaldehyde, salicylaldehyde, 2-methylbenzaldehyde, 2-chlorobenzaldehyde, 2-fluorobenzaldehyde, 2-benzaldehydesulfonate, 2-methoxybenzaldehyde, 1-hydroxy-2-naphthoaldehyde and n-hexylaldehyde Nocardioides sp. KP7 ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 53000, SDS-PAGE Nocardioides sp.

Synonyms

Synonyms Comment Organism
phdK
-
 Nocardioides sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Nocardioides sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
32
-
 NAD+ pH 9.4, 25°C Nocardioides sp.
39
-
 2-carboxybenzaldehyde pH 9.4, 25°C Nocardioides sp.
48
-
 2-nitrobenzaldehyde pH 9.4, 25°C Nocardioides sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Nocardioides sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Nocardioides sp.