Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Nocardioides sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, about 30% inhibition | Nocardioides sp. | |
EDTA | 10 mM, 28% inhibition | Nocardioides sp. | |
ethylenedioxybis(ethylamine)-N,N,N',N'-tetraacetic acid | 10 mM, 14% inhibition | Nocardioides sp. | |
Mg2+ | 10 mM, about 30% inhibition | Nocardioides sp. | |
Mn2+ | 10 mM, about 30% inhibition | Nocardioides sp. | |
additional information | less than 15% inhibition in presence of 10 mM Zn2+, Co2+ or Cu2+ | Nocardioides sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.083 | - |
NAD+ | pH 9.4, 25°C | Nocardioides sp. | |
0.1 | - |
2-carboxybenzaldehyde | pH 9.4, 25°C | Nocardioides sp. | |
2.8 | - |
2-carboxybenzaldehyde | pH 9.4, 25°C | Nocardioides sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
4 * 53000, SDS-PAGE | Nocardioides sp. |
205000 | - |
gel filtration | Nocardioides sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-carboxybenzaldehyde + NAD+ + H2O | Nocardioides sp. | the enzyme is involved in phenanthrene degradation | o-phthalic acid + NADH + H+ | - |
? | |
2-carboxybenzaldehyde + NAD+ + H2O | Nocardioides sp. KP7 | the enzyme is involved in phenanthrene degradation | o-phthalic acid + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nocardioides sp. | Q79EM7 | - |
- |
Nocardioides sp. KP7 | Q79EM7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Nocardioides sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:phenanthrene-grown cell | - |
Nocardioides sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-carboxybenzaldehyde + NAD+ + H2O | - |
Nocardioides sp. | o-phthalic acid + NADH + H+ | - |
? | |
2-carboxybenzaldehyde + NAD+ + H2O | the enzyme is involved in phenanthrene degradation | Nocardioides sp. | o-phthalic acid + NADH + H+ | - |
? | |
2-carboxybenzaldehyde + NAD+ + H2O | - |
Nocardioides sp. KP7 | o-phthalic acid + NADH + H+ | - |
? | |
2-carboxybenzaldehyde + NAD+ + H2O | the enzyme is involved in phenanthrene degradation | Nocardioides sp. KP7 | o-phthalic acid + NADH + H+ | - |
? | |
2-nitrobenzaldehyde + NAD+ + H2O | - |
Nocardioides sp. | ? | - |
? | |
2-nitrobenzaldehyde + NAD+ + H2O | - |
Nocardioides sp. KP7 | ? | - |
? | |
additional information | no activity with: 3-carboxybenzaldehyde, 4-carboxybenzaldehyde, benzaldehyde, salicylaldehyde, 2-methylbenzaldehyde, 2-chlorobenzaldehyde, 2-fluorobenzaldehyde, 2-benzaldehydesulfonate, 2-methoxybenzaldehyde, 1-hydroxy-2-naphthoaldehyde and n-hexylaldehyde | Nocardioides sp. | ? | - |
? | |
additional information | no activity with: 3-carboxybenzaldehyde, 4-carboxybenzaldehyde, benzaldehyde, salicylaldehyde, 2-methylbenzaldehyde, 2-chlorobenzaldehyde, 2-fluorobenzaldehyde, 2-benzaldehydesulfonate, 2-methoxybenzaldehyde, 1-hydroxy-2-naphthoaldehyde and n-hexylaldehyde | Nocardioides sp. KP7 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 53000, SDS-PAGE | Nocardioides sp. |
Synonyms | Comment | Organism |
---|---|---|
phdK | - |
Nocardioides sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Nocardioides sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
32 | - |
NAD+ | pH 9.4, 25°C | Nocardioides sp. | |
39 | - |
2-carboxybenzaldehyde | pH 9.4, 25°C | Nocardioides sp. | |
48 | - |
2-nitrobenzaldehyde | pH 9.4, 25°C | Nocardioides sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Nocardioides sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Nocardioides sp. |