Cloned (Comment) | Organism |
---|---|
construction of a betaine aldehyde dehydrogenase/choline dehydrogenase fusion protein and expression in Escherichia coli and Nicotiana tabacum. Escherichia coli cells expressing the fusion protein are able to grow to higher final densities and to accumulate more glycine betaine than cells expressing choline dehydrogenase alone | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
Betaine aldehyde | pH 7.5, 20°C, native betaine-aldehyde dehydrogenase or betaine aldehyde dehydrogenase/choline dehydrogenase fusion protein | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
117000 | - |
2 * 117000, betaine aldehyde dehydrogenase/choline dehydrogenase fusion protein, SDS-PAGE | Escherichia coli |
250000 | - |
betaine aldehyde dehydrogenase/choline dehydrogenase fusion protein, gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
betaine aldehyde + NAD+ + H2O | Escherichia coli | the osmoprotective compound glycine betaine is produced from choline by two enzymes. Choline dehydrogenase oxidizes choline to betaine aldehyde and then further to glycine betaine, while betaine aldehyde dehydrogenase facilitates the conversion of betaine aldehyde to glycine betaine | betaine + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
betaine aldehyde + NAD+ + H2O | - |
Escherichia coli | betaine + NADH + H+ | - |
? | |
betaine aldehyde + NAD+ + H2O | the osmoprotective compound glycine betaine is produced from choline by two enzymes. Choline dehydrogenase oxidizes choline to betaine aldehyde and then further to glycine betaine, while betaine aldehyde dehydrogenase facilitates the conversion of betaine aldehyde to glycine betaine | Escherichia coli | betaine + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 2 * 117000, betaine aldehyde dehydrogenase/choline dehydrogenase fusion protein, SDS-PAGE | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli |