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Literature summary for 1.2.1.8 extracted from

  • Diaz-Sanchez, A.; Gonzalez-Segura, L.; Rudino-Pinera, E.; Lira-Rocha, A.; Torres-Larios, A.; Munoz-Clares, R.
    Novel NADPH-cysteine covalent adduct found in the active site of an aldehyde dehydrogenase (2011), Biochem. J., 439, 443-452.
    View publication on PubMed
Search for more literature for 1.2.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
native and mutant enzyme C286A in complex with NADPH, hanging drop vapor diffusion method, using 85 mM HEPES/NaOH buffer, pH 7.5, 8.5% (v/v) propan-2-ol, 17% (w/v) PEG 4000, and 15% (v/v) glycerol Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
C286A mutant with reduced reactivity Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
NAD(P)H reversible inactivation Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
betaine aldehyde + NAD+ + H2O Pseudomonas aeruginosa
-
 glycine betaine + NADH + H+
-
 ir
betaine aldehyde + NADP+ + H2O Pseudomonas aeruginosa
-
 glycine betaine + NADPH + H+
-
 ir

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9HTJ1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
betaine aldehyde + NAD+ + H2O
-
 Pseudomonas aeruginosa glycine betaine + NADH + H+
-
 ir
betaine aldehyde + NADP+ + H2O
-
 Pseudomonas aeruginosa glycine betaine + NADPH + H+
-
 ir

Synonyms

Synonyms Comment Organism
BADH
-
 Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme can use NADP+ with similar efficiency to NAD+ Pseudomonas aeruginosa
NADP+ the enzyme can use NADP+ with similar efficiency to NAD+ Pseudomonas aeruginosa