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Literature summary for 1.2.1.8 extracted from

  • Chen, C.; Joo, J.C.; Brown, G.; Stolnikova, E.; Halavaty, A.S.; Savchenko, A.; Anderson, W.F.; Yakunin, A.F.
    Structure-based mutational studies of substrate inhibition of betaine aldehyde dehydrogenase BetB from Staphylococcus aureus (2014), Appl. Environ. Microbiol., 80, 3992-4002.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Gold cells Staphylococcus aureus

Protein Variants

Protein Variants Comment Organism
G234A the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme Staphylococcus aureus
G234S the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme Staphylococcus aureus
G234T the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme Staphylococcus aureus
H448F the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme Staphylococcus aureus
H448F/P449M the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme Staphylococcus aureus
H448F/P449M/Y450L the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme Staphylococcus aureus
H448F/Y450L the mutant demonstrates a complete loss of substrate inhibition Staphylococcus aureus
L161M/Q162M the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme Staphylococcus aureus
P449M/Y450L the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme Staphylococcus aureus
Q162M the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme Staphylococcus aureus
S290T the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme Staphylococcus aureus
V288D the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme Staphylococcus aureus
W456H the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme Staphylococcus aureus
Y450L the mutant with reduced catalytic efficiency demonstrates reduced substrate inhibition with betaine aldehyde compared to the wild type enzyme Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
Betaine aldehyde substrate inhibition at concentrations of betaine aldehyde as low as 0.15 mM Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
Betaine aldehyde mutant enzyme G234T, at pH 8.0 and 30°C Staphylococcus aureus
0.12
-
Betaine aldehyde mutant enzyme G234A, at pH 8.0 and 30°C Staphylococcus aureus
0.12
-
NAD+ mutant enzyme G234S, at pH 8.0 and 30°C Staphylococcus aureus
0.17
-
Betaine aldehyde wild type enzyme, at pH 8.0 and 30°C Staphylococcus aureus
0.17
-
Betaine aldehyde mutant enzyme G234S, at pH 8.0 and 30°C Staphylococcus aureus
0.26
-
NAD+ wild type enzyme, at pH 8.0 and 30°C Staphylococcus aureus
0.26
-
Betaine aldehyde mutant enzyme H448F, at pH 8.0 and 30°C Staphylococcus aureus
0.27
-
NAD+ mutant enzyme V288D, at pH 8.0 and 30°C Staphylococcus aureus
0.3
-
NAD+ mutant enzyme G234T, at pH 8.0 and 30°C Staphylococcus aureus
0.34
-
NAD+ mutant enzyme H448F/Y450L, at pH 8.0 and 30°C Staphylococcus aureus
0.38
-
NAD+ mutant enzyme W456H, at pH 8.0 and 30°C Staphylococcus aureus
0.41
-
NAD+ mutant enzyme S290T, at pH 8.0 and 30°C Staphylococcus aureus
0.42
-
NAD+ mutant enzyme H448F, at pH 8.0 and 30°C Staphylococcus aureus
0.43
-
NAD+ mutant enzyme P449M/Y450L, at pH 8.0 and 30°C Staphylococcus aureus
0.57
-
Betaine aldehyde mutant enzyme H448F/P449M, at pH 8.0 and 30°C Staphylococcus aureus
0.68
-
NAD+ mutant enzyme Q162M, at pH 8.0 and 30°C Staphylococcus aureus
0.71
-
NAD+ mutant enzyme L161M7Q162M, at pH 8.0 and 30°C Staphylococcus aureus
0.91
-
Betaine aldehyde mutant enzyme Q162M, at pH 8.0 and 30°C Staphylococcus aureus
1.01
-
NAD+ mutant enzyme Y450L, at pH 8.0 and 30°C Staphylococcus aureus
1.12
-
NAD+ mutant enzyme G234A, at pH 8.0 and 30°C Staphylococcus aureus
1.12
-
NAD+ mutant enzyme H448F/P449M, at pH 8.0 and 30°C Staphylococcus aureus
1.18
-
Betaine aldehyde mutant enzyme H448F/Y450L, at pH 8.0 and 30°C Staphylococcus aureus
1.92
-
Betaine aldehyde mutant enzyme Y450L, at pH 8.0 and 30°C Staphylococcus aureus
1.94
-
Betaine aldehyde mutant enzyme P449M/Y450L, at pH 8.0 and 30°C Staphylococcus aureus
2.16
-
Betaine aldehyde mutant enzyme L161M7Q162M, at pH 8.0 and 30°C Staphylococcus aureus
3.42
-
NAD+ mutant enzyme H448F/P449M/Y450L, at pH 8.0 and 30°C Staphylococcus aureus
4.61
-
Betaine aldehyde mutant enzyme H448F/P449M/Y450L, at pH 8.0 and 30°C Staphylococcus aureus
4.76
-
Betaine aldehyde mutant enzyme V288D, at pH 8.0 and 30°C Staphylococcus aureus
13.8
-
Betaine aldehyde mutant enzyme S290T, at pH 8.0 and 30°C Staphylococcus aureus
14.8
-
Betaine aldehyde mutant enzyme W456H, at pH 8.0 and 30°C Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
betaine aldehyde + NAD+ + H2O Staphylococcus aureus
-
betaine + NADH + 2 H+
-
ir

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
betaine aldehyde + NAD+ + H2O
-
Staphylococcus aureus betaine + NADH + 2 H+
-
ir

Synonyms

Synonyms Comment Organism
BADH
-
Staphylococcus aureus
BetB
-
Staphylococcus aureus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
Betaine aldehyde mutant enzyme G234T, at pH 8.0 and 30°C Staphylococcus aureus
0.25
-
Betaine aldehyde mutant enzyme V288D, at pH 8.0 and 30°C Staphylococcus aureus
0.94
-
Betaine aldehyde mutant enzyme H448F, at pH 8.0 and 30°C Staphylococcus aureus
1.09
-
Betaine aldehyde mutant enzyme H448F/Y450L, at pH 8.0 and 30°C Staphylococcus aureus
1.14
-
Betaine aldehyde mutant enzyme G234A, at pH 8.0 and 30°C Staphylococcus aureus
1.35
-
Betaine aldehyde mutant enzyme Y450L, at pH 8.0 and 30°C Staphylococcus aureus
1.63
-
Betaine aldehyde mutant enzyme S290T, at pH 8.0 and 30°C Staphylococcus aureus
1.8
-
Betaine aldehyde mutant enzyme H448F/P449M/Y450L, at pH 8.0 and 30°C Staphylococcus aureus
1.87
-
Betaine aldehyde mutant enzyme P449M/Y450L, at pH 8.0 and 30°C Staphylococcus aureus
2.92
-
Betaine aldehyde mutant enzyme G234S, at pH 8.0 and 30°C Staphylococcus aureus
3.2
-
Betaine aldehyde mutant enzyme W456H, at pH 8.0 and 30°C Staphylococcus aureus
4.06
-
Betaine aldehyde mutant enzyme H448F/P449M, at pH 8.0 and 30°C Staphylococcus aureus
4.46
-
Betaine aldehyde mutant enzyme L161M7Q162M, at pH 8.0 and 30°C Staphylococcus aureus
9.9
-
Betaine aldehyde mutant enzyme Q162M, at pH 8.0 and 30°C Staphylococcus aureus
11
-
Betaine aldehyde wild type enzyme, at pH 8.0 and 30°C Staphylococcus aureus

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme can use both NAD+ and NADP+ as cofactors, but the NAD+-dependent activity is at least 10 times higher Staphylococcus aureus
NADP+ the enzyme can use both NAD+ and NADP+ as cofactors, but the NAD+-dependent activity is at least 10 times higher Staphylococcus aureus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.34
-
Betaine aldehyde pH and temperature not specified in the publication Staphylococcus aureus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.053
-
Betaine aldehyde mutant enzyme V288D, at pH 8.0 and 30°C Staphylococcus aureus
0.12
-
Betaine aldehyde mutant enzyme S290T, at pH 8.0 and 30°C Staphylococcus aureus
0.22
-
Betaine aldehyde mutant enzyme W456H, at pH 8.0 and 30°C Staphylococcus aureus
0.39
-
Betaine aldehyde mutant enzyme H448F/P449M/Y450L, at pH 8.0 and 30°C Staphylococcus aureus
0.7
-
Betaine aldehyde mutant enzyme Y450L, at pH 8.0 and 30°C Staphylococcus aureus
0.91
-
Betaine aldehyde mutant enzyme G234T, at pH 8.0 and 30°C Staphylococcus aureus
0.92
-
Betaine aldehyde mutant enzyme H448F/Y450L, at pH 8.0 and 30°C Staphylococcus aureus
0.96
-
Betaine aldehyde mutant enzyme P449M/Y450L, at pH 8.0 and 30°C Staphylococcus aureus
2.06
-
Betaine aldehyde mutant enzyme L161M7Q162M, at pH 8.0 and 30°C Staphylococcus aureus
3.62
-
Betaine aldehyde mutant enzyme H448F, at pH 8.0 and 30°C Staphylococcus aureus
7.12
-
Betaine aldehyde mutant enzyme H448F/P449M, at pH 8.0 and 30°C Staphylococcus aureus
9.5
-
Betaine aldehyde mutant enzyme G234A, at pH 8.0 and 30°C Staphylococcus aureus
10.9
-
Betaine aldehyde mutant enzyme Q162M, at pH 8.0 and 30°C Staphylococcus aureus
17.2
-
Betaine aldehyde mutant enzyme G234S, at pH 8.0 and 30°C Staphylococcus aureus
64.7
-
Betaine aldehyde wild type enzyme, at pH 8.0 and 30°C Staphylococcus aureus