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Literature summary for 1.2.1.8 extracted from

  • Cruz-Valencia, R.; Arvizu-Flores, A.; Rosas-Rodriguez, J.; Valenzuela-Soto, E.
    Effect of the drug cyclophosphamide on the activity of porcine kidney betaine aldehyde dehydrogenase (2021), Mol. Cell. Biochem., 476, 1467-1475 .
    View publication on PubMed
Search for more literature for 1.2.1.8

Inhibitors

Inhibitors Comment Organism Structure
cyclophosphamide CTX, an antineoplastic and immuno-suppressant pre-drug, the enzyme loses 43% and 69% of its activity at 0.2 mM and 2.0 mM CTX, respectively, after 120 min. Docking of CTX to pkBADH and molecular modeling. The pseudofirst-order rate constant of inactivation (kobs) is dependent on CTX concentration only at low concentrations (0.2 and 0.6 mM CTX), and then the kobs between 1.0 and 2.0 mM show similar values. CTX can have access more easily to the catalytic cysteine when NAD+ opens the active site for substrate betaine aldehyde binding according to the kinetic mechanism, kinetics of inactivation of pkBADH by CTX in the presence of NADH, overview. Reversibility of inactivation, pkBADH inactivated with 2 mM CTX and incubated with DTT recovers 96% of its activity, and 93% with 2-mercaptoethanol, but in the presence of glutathione (GSH), the enzyme is not reactivated Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
betaine aldehyde + NAD+ + H2O Sus scrofa
-
 betaine + NADH + 2 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa A0A6M5K8J2
-
-

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
betaine aldehyde + NAD+ + H2O
-
 Sus scrofa betaine + NADH + 2 H+
-
 ?

Synonyms

Synonyms Comment Organism
BADH
-
 Sus scrofa
betaine aldehyde dehydrogenase
-
 Sus scrofa
pkBADH
-
 Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Sus scrofa

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Sus scrofa

General Information

General Information Comment Organism
evolution all BADHs known have cysteine in the active site involved in the aldehyde binding, whereas the porcine kidney enzyme (pkBADH) also has a neighborhood cysteine, both sensitive to oxidation Sus scrofa