Literature summary for 1.2.1.80 extracted from

Schirmer, A.; Rude, M.A.; Li, X.; Popova, E.; del Cardayre, S.B.
Microbial biosynthesis of alkanes (2010), Science, 329, 559-562.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of strain PCC7942 orf1593 and orf1594, the latter encoding an aldehde decarbonylase, both separately and together in Escherichia coli. Extracts of recombinant PCC7942_orf1594-expressing cells contain substantial quantities of evenchain fatty aldehydes and fatty alcohols, whereas coexpression of both PCC7942_orf1593 and orf1594 results in the production of odd chain alkanes and alkenes, as well as even-chain fatty aldehydes and fatty alcohols. Orf1593 and orf1594 appear to be part of a larger operon that contains a gene encoding a subunit of the acetyl-CoA carboxylase, accA, which is essential for growth	Synechococcus sp.

Cloned (Comment)

Organism

expression of strain PCC7942 orf1593 and orf1594, the latter encoding an aldehde decarbonylase, both separately and together in Escherichia coli. Extracts of recombinant PCC7942_orf1594-expressing cells contain substantial quantities of evenchain fatty aldehydes and fatty alcohols, whereas coexpression of both PCC7942_orf1593 and orf1594 results in the production of odd chain alkanes and alkenes, as well as even-chain fatty aldehydes and fatty alcohols. Orf1593 and orf1594 appear to be part of a larger operon that contains a gene encoding a subunit of the acetyl-CoA carboxylase, accA, which is essential for growth

Synechococcus sp.

Protein Variants

Protein Variants	Comment	Organism
additional information	replacement of the PC7942_orf1593 and 1594 orthologues in Synechocystis sp. PCC6803, where the orthologous genes apparently form only a bicistronic operon, with a kanamycin-resistance cassette. This replacement abolishes the presence of alkanes in the extracts of photoautoptrophically grown cells. PCC7942_orf 1593 and orf1594 coexpression in an Escherichia coli strain devoid of acyl-CoAs leads to very similar levels of hydrocarbon production, comparable to those in an Escherichia coli wild-type strain	Synechococcus sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0079	-	oleoyl-[acyl carrier protein]	pH and temperature not specified in the publication	Synechococcus elongatus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
fatty acyl-[acyl carrier protein] + NAD(P)H + H+	Synechococcus elongatus	kinetically preferred and likely the in vivo substrate	fatty aldehyde + acyl carrier protein + NAD(P)+	-	?
fatty acyl-[acyl carrier protein] + NAD(P)H + H+	Synechococcus elongatus PCC 7942	kinetically preferred and likely the in vivo substrate	fatty aldehyde + acyl carrier protein + NAD(P)+	-	?
hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+	Synechococcus sp.	acyl-ACP is kinetically preferred and is likely the in vivo substrate	hexadecanal + acyl-carrier protein + NAD(P)+	-	?
hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+	Synechococcus sp. PCC 7942	acyl-ACP is kinetically preferred and is likely the in vivo substrate	hexadecanal + acyl-carrier protein + NAD(P)+	-	?

Organism

Organism	UniProt	Comment	Textmining
Synechococcus elongatus	-	-	-
Synechococcus elongatus PCC 7942	-	-	-
Synechococcus sp.	-	orf1594 or sll0209	-
Synechococcus sp. PCC 7942	-	orf1594 or sll0209	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
fatty acyl-[acyl carrier protein] + NAD(P)H + H+	kinetically preferred and likely the in vivo substrate	Synechococcus elongatus	fatty aldehyde + acyl carrier protein + NAD(P)+	-	?
fatty acyl-[acyl carrier protein] + NAD(P)H + H+	kinetically preferred and likely the in vivo substrate	Synechococcus elongatus PCC 7942	fatty aldehyde + acyl carrier protein + NAD(P)+	-	?
hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+	substrate selectivity	Synechococcus sp.	hexadecanal + acyl-carrier protein + NAD(P)+	-	?
hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+	acyl-ACP is kinetically preferred and is likely the in vivo substrate	Synechococcus sp.	hexadecanal + acyl-carrier protein + NAD(P)+	-	?
hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+	substrate selectivity	Synechococcus sp. PCC 7942	hexadecanal + acyl-carrier protein + NAD(P)+	-	?
hexadecanoyl-[acyl-carrier protein] + NAD(P)H + H+	acyl-ACP is kinetically preferred and is likely the in vivo substrate	Synechococcus sp. PCC 7942	hexadecanal + acyl-carrier protein + NAD(P)+	-	?
additional information	does not reduce acyl-CoA	Synechococcus elongatus	?	-	?
additional information	does not reduce acyl-CoA	Synechococcus elongatus PCC 7942	?	-	?
oleoyl-[acyl carrier protein] + NAD(P)H + H+	-	Synechococcus elongatus	oleic aldehyde + acyl carrier protein + NAD(P)+	-	?
oleoyl-[acyl carrier protein] + NAD(P)H + H+	-	Synechococcus elongatus PCC 7942	oleic aldehyde + acyl carrier protein + NAD(P)+	-	?

Synonyms

Synonyms	Comment	Organism
AAR	-	Synechococcus sp.
AAR	-	Synechococcus elongatus
acyl-ACP reductase	-	Synechococcus sp.
acyl-ACP reductase	-	Synechococcus elongatus
acyl-acyl carrier protein reductase	-	Synechococcus elongatus
fatty aldehyde-generating acyl-ACP reductase	-	Synechococcus sp.

Cofactor

Cofactor	Comment	Organism	Structure
NAD(P)H	-	Synechococcus sp.
NAD(P)H	-	Synechococcus elongatus