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Literature summary for 1.2.1.80 extracted from

  • Warui, D.M.; Pandelia, M.E.; Rajakovich, L.J.; Krebs, C.; Bollinger, J.M.; Booker, S.J.
    Efficient delivery of long-chain fatty aldehydes from the Nostoc punctiforme acyl-acyl carrier protein reductase to its cognate aldehyde-deformylating oxygenase (2015), Biochemistry, 54, 1006-1015.
    View publication on PubMed
Search for more literature for 1.2.1.80

Cloned(Commentary)

Cloned (Comment) Organism
expession in Escherichia coli Nostoc punctiforme

Inhibitors

Inhibitors Comment Organism Structure
K+ K+, NH4+, and Rb+ at 0.2 M activate up to 20fold. Inhibitory at higher concentrations Nostoc punctiforme
NH4+ K+, NH4+, and Rb+ at 0.2 M activate up to 20fold. Inhibitory at higher concentrations Nostoc punctiforme
Rb+ K+, NH4+, and Rb+ at 0.2 M activate up to 20fold. Inhibitory at higher concentrations Nostoc punctiforme

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
 stearoyl-[acyl-carrier protein] presence of potassium ions, pH 7.6, 37°C Nostoc punctiforme
0.027
-
 stearoyl-[acyl-carrier protein] pH 7.6, 37°C Nostoc punctiforme

Metals/Ions

Metals/Ions Comment Organism Structure
K+ K+, NH4+, and Rb+ at 0.2 M activate up to 20fold. Inhibitory at hiher concentrations Nostoc punctiforme
NH4+ K+, NH4+, and Rb+ at 0.2 M activate up to 20fold. Inhibitory at hiher concentrations Nostoc punctiforme
Rb+ K+, NH4+, and Rb+ at 0.2 M activate up to 20fold. Inhibitory at hiher concentrations Nostoc punctiforme

Organism

Organism UniProt Comment Textmining
Nostoc punctiforme B2J1M0
-
-
Nostoc punctiforme ATCC 29133 B2J1M0
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation codon-optimized gene for expression in Escherichia coli Nostoc punctiforme
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
stearoyl-[acyl-carrier protein] + NAD(P)H + H+
-
 Nostoc punctiforme stearylaldehyde + an [acyl-carrier protein] + NAD(P)+ the enzyme specifically transfers the pro-R hydride of NADPH to the Cys294-thioester intermediate to afford its aldehyde product ?
stearoyl-[acyl-carrier protein] + NAD(P)H + H+
-
 Nostoc punctiforme ATCC 29133 stearylaldehyde + an [acyl-carrier protein] + NAD(P)+ the enzyme specifically transfers the pro-R hydride of NADPH to the Cys294-thioester intermediate to afford its aldehyde product ?

Synonyms

Synonyms Comment Organism
Npun_R1710
-
 Nostoc punctiforme

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0003
-
 stearoyl-[acyl-carrier protein] pH 7.6, 37°C Nostoc punctiforme
0.0028
-
 stearoyl-[acyl-carrier protein] presence of potassium ions, pH 7.6, 37°C Nostoc punctiforme

Cofactor

Cofactor Comment Organism Structure
NAD(P)H
-
 Nostoc punctiforme

General Information

General Information Comment Organism
physiological function enzyme and aldehyde-deformylating oxygenase ADO form a tight isolable complex with a Kd of 3 microM. When the aldehyde substrate is supplied to ADO by acyl-acyl carrier protein reductase, efficient in vitro turnover is observed in the absence of solubilizing agents. Catalysis by acyl-acyl carrier protein reductase proceeds via formation of a covalent intermediate involving residue C294. The enzyme specifically transfers the pro-R hydride of NADPH to the Cys294-thioester intermediate to afford its aldehyde product Nostoc punctiforme