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Literature summary for 1.2.1.80 extracted from

  • Gao, Y.; Zhang, H.; Fan, M.; Jia, C.; Shi, L.; Pan, X.; Cao, P.; Zhao, X.; Chang, W.; Li, M.
    Structural insights into catalytic mechanism and product delivery of cyanobacterial acyl-acyl carrier protein reductase (2020), Nat. Commun., 11, 1525 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.1.80

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Synechococcus elongatus

Crystallization (Commentary)

Crystallization (Comment) Organism
ligand-free enzyme and three complex structures in which the enzyme binds to aldehyde-deformylating oxygenase and various ligands (stearoyl-CoA, NADPH, thioester), hanging drop vapor diffusion method, using 0.1 M citric acid, pH6.0, and 1 M (NH4)2SO4 (ligand-free enzyme) or 0.1 M HEPES, pH 7.5, and 20% (w/v) PEG8000 (enzyme in complex with ligands) Synechococcus elongatus
sitting-drop vapor diffusion method at 18°C, structure of the ligand-free enzyme, and three acyl-acyl carrier protein reductase/aldehyde-deformylating oxygenase complex structures in which acyl-acyl carrier protein reductase bind various ligands Synechococcus elongatus

Protein Variants

Protein Variants Comment Organism
C294S the mutant avoids spontaneous formation of the acyl-enzyme intermediate Synechococcus elongatus
K80A the mutant does not bind to aldehyde-deformylating oxygenase Synechococcus elongatus
R73A the mutant does not bind to aldehyde-deformylating oxygenase Synechococcus elongatus
R79A the mutant does not bind to aldehyde-deformylating oxygenase Synechococcus elongatus
Y247A the inactive mutant does not bind to aldehyde-deformylating oxygenase Synechococcus elongatus
Y247F the mutant shows strongly increased activity compared to the wild type enzyme Synechococcus elongatus

Organism

Organism UniProt Comment Textmining
Synechococcus elongatus Q54765
-
-
Synechococcus elongatus PCC 7942 Q54765
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni affinity column chromatography Synechococcus elongatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
stearoyl-CoA + NADPH + H+
-
 Synechococcus elongatus octadecanal + CoA + NADP+
-
 ?
stearoyl-CoA + NADPH + H+
-
 Synechococcus elongatus PCC 7942 octadecanal + CoA + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
AAR
-
 Synechococcus elongatus
acyl-ACP reductase
-
 Synechococcus elongatus
acyl-acyl carrier protein (acyl-ACP) reductase
-
 Synechococcus elongatus
acyl-acyl carrier protein reductase
-
 Synechococcus elongatus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Synechococcus elongatus

General Information

General Information Comment Organism
physiological function the enzyme is involved in an alkane biosynthesis pathway. It forms a complex with aldehyde-deformylating oxygenase that efficiently converts long chain fatty acyl-ACP/fatty acyl-CoA into hydrocarbon Synechococcus elongatus