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Literature summary for 1.2.1.84 extracted from
- Fatty alcohol production in engineered E. coli expressing Marinobacter fatty acyl-CoA reductases (2013), Appl. Microbiol. Biotechnol., 97, 7061-7071 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene maqu_2220, recombinant expression in Escherichia coli leading to fatty alcohol production, coexpression with gene fadD in Escherichia coli strain BL21DELTAfadE | Marinobacter nauticus |
| gene maqu_2507, recombinant expression in Escherichia coli leading to fatty alcohol production, coexpression with gene fadD in Escherichia coli strain BL21DELTAfadE | Marinobacter nauticus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | de novo fatty alcohol production in Escherichia coli strain AL338 containing plasmidpAL144 or strain AL306, with fadD gene, encoding fatty acyl-CoA synthase, and maqu_2507 gene, method optimization. Biosynthetic pathways of fatty alcohols in genetically engineered Escherichia coli, overview. The amount of fatty alcohols in AL339 is significantly increased to 218.2 mg/l, 9fold higher than that of the control strain AL338. The percentage of medium chain fatty alcohols (12:1, 12:0, 14:1, 14:0, and 16:1) in total fatty alcohols are dramatically increased, especially the C12 and C14 fatty alcohols, accounting for 89% of total fatty alcohols. At the same time, the percentage of C16 and C18 fatty alcohols drops drastically from more than 60% to about 9% and from more than 15% to less than 2%, respectively | Marinobacter nauticus |
| additional information | de novo fatty alcohol production in Escherichia coli strain AL379 containing plasmidpAL144 or in strain AL307, with fadD gene, encoding fatty acyl-CoA synthase, and maqu_2220 gene, method optimization. Biosynthetic pathways of fatty alcohols in genetically engineered Escherichia coli, composition and amounts of fatty alcohols, overview | Marinobacter nauticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| a long-chain acyl-CoA + 2 NADPH + 2 H+ | Marinobacter nauticus | - |
a long-chain alcohol + 2 NADP+ + coenzyme A | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Marinobacter nauticus | A1U2T0 | - |
- |
| Marinobacter nauticus | A1U3L3 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| a long-chain acyl-CoA + 2 NADPH + 2 H+ | - |
Marinobacter nauticus | a long-chain alcohol + 2 NADP+ + coenzyme A | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| fatty acyl-CoA reductase | - |
Marinobacter nauticus |
| male sterility C-terminal domain | UniProt | Marinobacter nauticus |
| Maqu_2220 | - |
Marinobacter nauticus |
| Maqu_2507 | - |
Marinobacter nauticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
in vivo assay at | Marinobacter nauticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
in vivo assay at | Marinobacter nauticus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Marinobacter nauticus | |
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Release 2023.1 (January 2023)
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