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Literature summary for 1.2.1.86 extracted from
- Citral dehydrogenase involved in geraniol oxidation pathway purification, characterization and kinetic studies from Persicaria minor (Polygonum minus Huds.) (2018), J. Plant Biochem. Biotechnol., 27, 1-12 .
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.31 | - |
NAD+ | isoform citral dehydrogenase II, at pH 9.5 and 35°C | Persicaria minor |  |
| 0.33 | - |
NAD+ | isoform citral dehydrogenase I, at pH 9.5 and 35°C | Persicaria minor | |
| 0.45 | - |
citral | isoform citral dehydrogenase I, at pH 9.5 and 35°C | Persicaria minor | |
| 0.56 | - |
citral | isoform citral dehydrogenase II, at pH 9.5 and 35°C | Persicaria minor | |
| 0.83 | - |
trans-cinnamaldehyde | isoform citral dehydrogenase I, at pH 9.5 and 35°C | Persicaria minor | |
| 1.11 | - |
alpha-methyl cinnamaldehyde | isoform citral dehydrogenase I, at pH 9.5 and 35°C | Persicaria minor | |
| 1.25 | - |
trans-cinnamaldehyde | isoform citral dehydrogenase II, at pH 9.5 and 35°C | Persicaria minor | |
| 1.54 | - |
alpha-methyl cinnamaldehyde | isoform citral dehydrogenase II, at pH 9.5 and 35°C | Persicaria minor | |
| 2.5 | - |
S-perillyl aldehyde | isoform citral dehydrogenase II, at pH 9.5 and 35°C | Persicaria minor | |
| 3.33 | - |
S-perillyl aldehyde | isoform citral dehydrogenase I, at pH 9.5 and 35°C | Persicaria minor |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
isoform citral dehydrogenase I, gel filtration | Persicaria minor |
| 110000 | - |
isoform citral dehydrogenase II, gel filtration | Persicaria minor |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| citral + NAD+ + H2O | Persicaria minor | - |
geranic acid + NADH + H+ | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Persicaria minor | - |
synonym Polygonum minus | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Toyopearl GigaCapQ-650 M column chromatography, Toyopearl Giga Cap S-650 M column chromatography, Toyopearl AF-Blue-650 M column chromatography and TSK gel G3000SW gel filtration | Persicaria minor |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Persicaria minor | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.4 | - |
crude extract, at pH 9.5 and 35°C | Persicaria minor |
| 138 | - |
isoform citral dehydrogenase II, after 31.5fold purification, at pH 9.5and 35°C | Persicaria minor |
| 241.2 | - |
isoform citral dehydrogenase I, after 54.9fold purification, at pH 9.5 and 35°C | Persicaria minor |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-methyl cinnamaldehyde + NAD+ + H2O | isoform citral dehydrogenase I shows 62% activity compared to citral, isoform citral dehydrogenase II shows 85% activity compared to citral | Persicaria minor | alpha-methyl cinnamic acid + NADH + H+ | - |
? | |
| citral + NAD+ + H2O | - |
Persicaria minor | geranic acid + NADH + H+ | - |
ir | |
| citral + NAD+ + H2O | 100% activity | Persicaria minor | geranic acid + NADH + H+ | - |
ir | |
| geranial + NAD+ + H2O | - |
Persicaria minor | geranic acid + NADH + H+ | - |
? | |
| additional information | the enzyme shows no reaction with NADP+. No reduction reaction is observed either with NADH or NADPH as a coenzyme | Persicaria minor | ? | - |
- |
|
| S-perillyl aldehyde + NAD+ + H2O | isoform citral dehydrogenase I shows 37% activity compared to citral, isoform citral dehydrogenase II shows 54% activity compared to citral | Persicaria minor | perillic acid + NADH + H+ | - |
? | |
| trans-cinnamaldehyde + NAD+ + H2O | isoform citral dehydrogenase I shows 73% activity compared to citral, isoform citral dehydrogenase II shows 87% activity compared to citral | Persicaria minor | cinnamic acid + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | 1 * 62000 + 1 * 60000, isoform citral dehydrogenase I, SDS-PAGE | Persicaria minor |
| heterodimer | 1 * 65000 + 1 * 58000, isoform citral dehydrogenase II, SDS-PAGE | Persicaria minor |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| citral dehydrogenase I | isoform | Persicaria minor |
| citral dehydrogenase II | isoform | Persicaria minor |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Persicaria minor |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
20% of citral dehydrogenase II activity remains while citral dehydrogenase I is completely inactivated at 60°C | Persicaria minor |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Persicaria minor |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | below pH 8.0, both isoenzymes show less than 50% of relative activity while complete inactivation is observed at pH 5.0-6.0 and pH 10.5-11.0 | Persicaria minor |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Persicaria minor | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Persicaria minor | isoform citral dehydrogenase II, isoelectric focusing | - |
4.2 |
| Persicaria minor | isoform citral dehydrogenase I, isoelectric focusing | - |
7.1 |
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Release 2023.1 (January 2023)
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