Cloned (Comment) | Organism |
---|---|
bphI and bphJ cloned into the plasmids pBTL4-T7 and pET28a | Paraburkholderia xenovorans |
Protein Variants | Comment | Organism |
---|---|---|
I195F | created by site-specific mutagenesis, no significant reduction of channeling efficiency of the enzyme complex toward acetaldehyde or propionaldehyde | Paraburkholderia xenovorans |
I195L | created by site-specific mutagenesis | Paraburkholderia xenovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 3 | propionaldehyde | wild-type protein, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
7.4 | - |
acetaldehyde | I195L mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
7.7 | - |
propionaldehyde | I195L mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
24 | - |
acetaldehyde | wild-type protein, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
35 | - |
acetaldehyde | I195F mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
48 | - |
propionaldehyde | I195F mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paraburkholderia xenovorans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
BphI and BphJ form a stable complex as they bind and coelute from Ni2+-NTA column, after purification N-terminal histidine tag of BphJ is proteolytically cleaved by thrombin digestion | Paraburkholderia xenovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + CoA + NAD+ | - |
Paraburkholderia xenovorans | acetyl-CoA + NADH + H+ | - |
? | |
propionaldehyde + CoA + NAD+ | - |
Paraburkholderia xenovorans | propanoyl-CoA + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BphJ | - |
Paraburkholderia xenovorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.9 | - |
propionaldehyde | I195F mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
16 | - |
acetaldehyde | I195F mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
16.3 | - |
propionaldehyde | wild-type protein, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
17.2 | - |
acetaldehyde | wild-type protein, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
42 | - |
acetaldehyde | I195L mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans | |
46 | - |
propionaldehyde | I195L mutant, steady state, pH 8.0, 25°C | Paraburkholderia xenovorans |
General Information | Comment | Organism |
---|---|---|
metabolism | enzyme is part of the BphI-BphJ-complex, an aldolase-dehydrogenase-complex from the polychlorinated biphenyl degradation pathway | Paraburkholderia xenovorans |