Crystallization (Comment) | Organism |
---|---|
- |
Lysinibacillus sphaericus |
General Stability | Organism |
---|---|
NADP+ stabilizes | Lysinibacillus sphaericus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0095 | - |
NADP+ | - |
Lysinibacillus sphaericus | |
0.42 | - |
1-pyrroline-5-carboxylate | with NADP+ | Lysinibacillus sphaericus | |
0.75 | - |
1-pyrroline-5-carboxylate | with NAD+ | Lysinibacillus sphaericus | |
2.3 | - |
NAD+ | - |
Lysinibacillus sphaericus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Lysinibacillus sphaericus |
100000 | - |
gel filtration | Lysinibacillus sphaericus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
strains IFO 12983, ATCC 8005 and ATCC 21365 | - |
Geobacillus thermodenitrificans | - |
strains ATCC 39492 and DSM 465 | - |
Lysinibacillus sphaericus | - |
- |
- |
[Bacillus] caldotenax | - |
strain DSM 406 | - |
Purification (Comment) | Organism |
---|---|
- |
Lysinibacillus sphaericus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.6 | - |
strain ATCC 21365 | Geobacillus stearothermophilus |
2.6 | - |
strain ATCC 39492 | Geobacillus thermodenitrificans |
2.7 | - |
- |
[Bacillus] caldotenax |
3.3 | - |
strain DSM 465 | Geobacillus thermodenitrificans |
7.1 | - |
strain ATCC 8005 | Geobacillus stearothermophilus |
8 | - |
strain IFO 12983 | Geobacillus stearothermophilus |
34.1 | - |
- |
Lysinibacillus sphaericus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-pyrroline-5-carboxylate + NAD+ + H2O | - |
Geobacillus stearothermophilus | L-glutamate + NADH | - |
ir | |
1-pyrroline-5-carboxylate + NAD+ + H2O | - |
[Bacillus] caldotenax | L-glutamate + NADH | - |
ir | |
1-pyrroline-5-carboxylate + NAD+ + H2O | - |
Geobacillus thermodenitrificans | L-glutamate + NADH | - |
ir | |
1-pyrroline-5-carboxylate + NAD+ + H2O | highly specific | Lysinibacillus sphaericus | L-glutamate + NADH | - |
ir | |
1-pyrroline-5-carboxylate + NAD+ + H2O | - |
Lysinibacillus sphaericus | L-glutamate + NADH + H+ | - |
r | |
1-pyrroline-5-carboxylate + NADP+ + H2O | - |
Lysinibacillus sphaericus | L-glutamate + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, SDS-PAGE | Lysinibacillus sphaericus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
cofactor NAD+ | Lysinibacillus sphaericus |
45 | - |
cofactor NADP+ | Lysinibacillus sphaericus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
15 min, 40% loss of activity in presence of NAD+, 2% loss of activity in presence of NADP+ | Lysinibacillus sphaericus |
40 | - |
15 min, 75% loss of activity in presence of NAD+, 10% loss of activity in presence of NADP+ | Lysinibacillus sphaericus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Lysinibacillus sphaericus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | cofactor NAD+ | Lysinibacillus sphaericus |
5.5 | 8 | cofactor NADP+ | Lysinibacillus sphaericus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Geobacillus stearothermophilus | |
NAD+ | - |
Lysinibacillus sphaericus | |
NAD+ | - |
[Bacillus] caldotenax | |
NAD+ | - |
Geobacillus thermodenitrificans | |
NADP+ | 8.1times higher activity compared to NAD+ | Lysinibacillus sphaericus |