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Literature summary for 1.2.1.89 extracted from

  • Steffler, F.; Guterl, J.K.; Sieber, V.
    Improvement of thermostable aldehyde dehydrogenase by directed evolution for application in Synthetic Cascade Biomanufacturing (2013), Enzyme Microb. Technol., 53, 307-314.
    View publication on PubMed
Search for more literature for 1.2.1.89

Cloned(Commentary)

Cloned (Comment) Organism
-
 Thermoplasma acidophilum
expressed in Escherichia coli BL21(DE3) cells Thermoplasma acidophilum

Protein Variants

Protein Variants Comment Organism
34M/Y399C/S405N the mutations enhance enzyme activity with the cofactor NAD+ by a factor of 55.7 Thermoplasma acidophilum
F34L the mutation enhances enzyme activity with the cofactor NAD+ by a factor of 3.2 Thermoplasma acidophilum
F34M/D372N/S405N the mutations enhance enzyme activity with the cofactor NAD+ by a factor of 7.2 Thermoplasma acidophilum
F34M/D372N/Y399C/S405N the mutations enhance enzyme activity with the cofactor NAD+ by a factor of 35.1 Thermoplasma acidophilum
F34M/S405N enhances enzyme activity with the cofactor NAD+ by a factor of eight Thermoplasma acidophilum
F34M/S405N the mutations enhance enzyme activity with the cofactor NAD+ by a factor of 8.5 while impacts on enzyme stability and substrate specificity are negligible Thermoplasma acidophilum
F34M/W271R/S405N the mutations enhance enzyme activity with the cofactor NAD+ by a factor of 22.6 Thermoplasma acidophilum
F34M/W271S/S405N the mutations enhance enzyme activity with the cofactor NAD+ by a factor of 42.5 Thermoplasma acidophilum
F34M/W271S/Y399C/S405N the mutations enhance enzyme activity with the cofactor NAD+ by a factor of 1.1 Thermoplasma acidophilum
F34M/Y399C/S405N enhances enzyme activity with the cofactor NAD+ by a factor of eight and enhances solubilty Thermoplasma acidophilum
S405C the mutation enhances enzyme activity with the cofactor NAD+ by a factor of 6 Thermoplasma acidophilum
S405N the mutation enhances enzyme activity with the cofactor NAD+ by a factor of 7.4 Thermoplasma acidophilum
W271S the mutations enhance enzyme activity with the cofactor NAD+ by a factor of 17.1 Thermoplasma acidophilum
Y399C enhances the protein solubility after recombinant expression in Escherichia coli 6fold Thermoplasma acidophilum
Y399C the mutation enhances the protein solubility after recombinant expression in Escherichia coli 6fold and enhances enzyme activity with the cofactor NAD+ by a factor of 6.4 Thermoplasma acidophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
 NADP+ wild-type, pH 7.0, 50°C Thermoplasma acidophilum
0.031
-
 NADP+ mutant F34M/Y399C/S405N, pH 7.0, 50°C Thermoplasma acidophilum
0.036
-
 NADP+ mutant F34M/S405N, pH 7.0, 50°C Thermoplasma acidophilum
16.7
-
 NAD+ mutant F34M/Y399C/S405N, pH 7.0, 50°C Thermoplasma acidophilum
16.7
-
 NAD+ mutant enzyme F34M/Y399C/S405N, at pH 7.0 and 50°C Thermoplasma acidophilum
17.6
-
 NAD+ mutant F34M/S405N, pH 7.0, 50°C Thermoplasma acidophilum
17.6
-
 NAD+ mutant enzyme F34M/S405N, at pH 7.0 and 50°C Thermoplasma acidophilum
22.7
-
 NAD+ wild-type, pH 7.0, 50°C Thermoplasma acidophilum
22.7
-
 NAD+ wild type enzyme, at pH 7.0 and 50°C Thermoplasma acidophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 x * 55000, SDS-PAGE Thermoplasma acidophilum

Organic Solvent Stability

Organic Solvent Comment Organism
isobutanol wild-type, 50% residual activity at 3.1%, mutant F34M/S405N at 3.6%, mutant F34M/Y399C/S405N at 3.5%, respectively Thermoplasma acidophilum

Organism

Organism UniProt Comment Textmining
Thermoplasma acidophilum Q9HK01
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap column chromatography Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde + NAD+ NAD+ is a very poor cofactor for wild-type Thermoplasma acidophilum D-glycerate + NADH + H+
-
 ?
D-glyceraldehyde + NADP+ + H2O
-
 Thermoplasma acidophilum D-glycerate + NADPH + H+
-
 ?
glyceraldehyde + NAD+ + H2O
-
 Thermoplasma acidophilum glycerate + NADH + H+
-
 ?
additional information no activity with acetaldehyde and isobutyraldehyde Thermoplasma acidophilum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Thermoplasma acidophilum

Synonyms

Synonyms Comment Organism
ALDH
-
 Thermoplasma acidophilum
Ta0809
-
 Thermoplasma acidophilum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.64
-
 NAD+ wild-type, pH 7.0, 50°C Thermoplasma acidophilum
1.64
-
 NAD+ wild type enzyme, at pH 7.0 and 50°C Thermoplasma acidophilum
10.54
-
 NAD+ mutant F34M/Y399C/S405N, pH 7.0, 50°C Thermoplasma acidophilum
10.54
-
 NAD+ mutant enzyme F34M/Y399C/S405N, at pH 7.0 and 50°C Thermoplasma acidophilum
10.99
-
 NAD+ mutant F34M/S405N, pH 7.0, 50°C Thermoplasma acidophilum
10.99
-
 NAD+ mutant enzyme F34M/S405N, at pH 7.0 and 50°C Thermoplasma acidophilum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Thermoplasma acidophilum
NADH poor cofoactor Thermoplasma acidophilum
NADPH NADPH is preferred over NADH Thermoplasma acidophilum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.07
-
 NAD+ wild-type, pH 7.0, 50°C Thermoplasma acidophilum
0.07
-
 NAD+ wild type enzyme, at pH 7.0 and 50°C Thermoplasma acidophilum
0.62
-
 NAD+ mutant F34M/S405N, pH 7.0, 50°C Thermoplasma acidophilum
0.62
-
 NAD+ mutant enzyme F34M/S405N, at pH 7.0 and 50°C Thermoplasma acidophilum
0.63
-
 NAD+ mutant F34M/Y399C/S405N, pH 7.0, 50°C Thermoplasma acidophilum
0.63
-
 NAD+ mutant enzyme F34M/Y399C/S405N, at pH 7.0 and 50°C Thermoplasma acidophilum