Literature summary for 1.2.1.89 extracted from

Wu, X.; Xu, L.; Yan, M.
A new NAD(+)-dependent glyceraldehyde dehydrogenase obtained by rational design of l-lactaldehyde dehydrogenase from Escherichia coli (2016), Biosci. Biotechnol. Biochem., 80, 2306-2310.

Search for more literature for 1.2.1.89

Protein Variants

Protein Variants	Comment	Organism
N286E	engineering of L-lactaldehyde dehydrogenase. Contrary to wild-type, mutant is able to oxidate glyceraldehyde	Escherichia coli
N286H	engineering of L-lactaldehyde dehydrogenase. Contrary to wild-type, mutant is able to oxidate glyceraldehyde	Escherichia coli
N286T	engineering of L-lactaldehyde dehydrogenase. Contrary to wild-type, mutant is able to oxidate glyceraldehyde. Compared to L-lactaldehyde, N286T has a one-third lower Km value to glyceraldehyde.	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.14	-	D-glyceraldehyde	mutant N286H, pH 7.0, 37°C	Escherichia coli
1.97	-	D-glyceraldehyde	mutant N286H, pH 7.0, 37°C	Escherichia coli
2.2	-	D-glyceraldehyde	mutant N286H, pH 7.0, 37°C	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P25553	cf. EC 1.2.1.22	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.08	-	mutant N286H, pH 7.0, 37°C	Escherichia coli
0.13	-	mutant N286H, pH 7.0, 37°C	Escherichia coli
1.1	-	mutant N286H, pH 7.0, 37°C	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glyceraldehyde + NAD+ + H2O	activity of mutants N286E, N286H, N286T. No substrate for wild-type	Escherichia coli	D-glycerate + NADH + H+	-	?

Synonyms

Synonyms	Comment	Organism
AldA	-	Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
55	-	1 h, 70% residual activity for mutant N286T	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
69.6	-	D-glyceraldehyde	mutant N286H, pH 7.0, 37°C	Escherichia coli
113.1	-	D-glyceraldehyde	mutant N286H, pH 7.0, 37°C	Escherichia coli
957	-	D-glyceraldehyde	mutant N286H, pH 7.0, 37°C	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	mutants N286E, N286H, N286T, but not wild-type show activtiy with NAD+	Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
31.64	-	D-glyceraldehyde	mutant N286H, pH 7.0, 37°C	Escherichia coli
99.2	-	D-glyceraldehyde	mutant N286H, pH 7.0, 37°C	Escherichia coli
485.8	-	D-glyceraldehyde	mutant N286H, pH 7.0, 37°C	Escherichia coli

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Release 2023.1 (January 2023)