BRENDA - Enzyme Database show
show all sequences of 1.2.1.9

Engineered nonphosphorylating gylceraldehyde 3-phosphate dehydrogenase at position 268 binds hydroxylamine and hydrazine as acyl acceptors

Marchal, S.; Branlant, G.; Eur. J. Biochem. 268, 5764-5770 (2001)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
E268A
attacking water molecule in the hydrolysis process is poorly activated, can be overcome by the nulceophiles hydrazin and hydroxylamine
Streptococcus mutans
E268Q
attacking water molecule in the hydrolysis process is poorly activated, can be overcome by the nulceophiles hydrazin and hydroxylamine
Streptococcus mutans
Inhibitors
Inhibitors
Commentary
Organism
Structure
hydrazin
protonated and neutral form, compete with the attacking water molecule for the binding site E268, inhibition mechanism
Streptococcus mutans
hydroxylamine
protonated and neutral form, compete with the attacking water molecule for the binding site E268, inhibition mechanism
Streptococcus mutans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-glyceraldehyde-3-phosphate + NADP+
Streptococcus mutans
-
D-3-phosphoglycerate + NADPH
-
-
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptococcus mutans
-
-
-
Reaction
Reaction
Commentary
Organism
D-glyceraldehyde 3-phosphate + NADP+ + H2O = 3-phospho-D-glycerate + NADPH + 2 H+
two-step mechanism and kinetics
Streptococcus mutans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glyceraldehyde-3-phosphate + NADP+
-
288346
Streptococcus mutans
D-3-phosphoglycerate + NADPH
-
-
-
ir
D-glyceraldehyde-3-phosphate + NADP+
two-step mechanism
288346
Streptococcus mutans
D-3-phosphoglycerate + NADPH
-
-
-
ir
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Streptococcus mutans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
assay at
Streptococcus mutans
pH Range
pH Minimum
pH Maximum
Commentary
Organism
additional information
-
steady-state rate constant for mutant E268Q and E268A is pH-dependent
Streptococcus mutans
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Streptococcus mutans
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.7
-
hydroxylamine
wild-type enzyme
Streptococcus mutans
6.3
-
hydrazin
wild-type enzyme
Streptococcus mutans
57
-
hydroxylamine
mutant E268Q
Streptococcus mutans
95
-
hydroxylamine
mutant E268A
Streptococcus mutans
109
-
hydrazin
mutant E268A
Streptococcus mutans
254
-
hydrazin
mutant E268Q
Streptococcus mutans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Streptococcus mutans
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
E268A
attacking water molecule in the hydrolysis process is poorly activated, can be overcome by the nulceophiles hydrazin and hydroxylamine
Streptococcus mutans
E268Q
attacking water molecule in the hydrolysis process is poorly activated, can be overcome by the nulceophiles hydrazin and hydroxylamine
Streptococcus mutans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
hydrazin
protonated and neutral form, compete with the attacking water molecule for the binding site E268, inhibition mechanism
Streptococcus mutans
hydroxylamine
protonated and neutral form, compete with the attacking water molecule for the binding site E268, inhibition mechanism
Streptococcus mutans
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.7
-
hydroxylamine
wild-type enzyme
Streptococcus mutans
6.3
-
hydrazin
wild-type enzyme
Streptococcus mutans
57
-
hydroxylamine
mutant E268Q
Streptococcus mutans
95
-
hydroxylamine
mutant E268A
Streptococcus mutans
109
-
hydrazin
mutant E268A
Streptococcus mutans
254
-
hydrazin
mutant E268Q
Streptococcus mutans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-glyceraldehyde-3-phosphate + NADP+
Streptococcus mutans
-
D-3-phosphoglycerate + NADPH
-
-
ir
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glyceraldehyde-3-phosphate + NADP+
-
288346
Streptococcus mutans
D-3-phosphoglycerate + NADPH
-
-
-
ir
D-glyceraldehyde-3-phosphate + NADP+
two-step mechanism
288346
Streptococcus mutans
D-3-phosphoglycerate + NADPH
-
-
-
ir
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Streptococcus mutans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
assay at
Streptococcus mutans
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
additional information
-
steady-state rate constant for mutant E268Q and E268A is pH-dependent
Streptococcus mutans
Other publictions for EC 1.2.1.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739907
Komati Reddy
Metabolic engineering of an AT ...
Clostridium acetobutylicum
Appl. Environ. Microbiol.
81
1996-2005
2015
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5
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1
1
-
-
-
724471
Ito
Engineering the allosteric pro ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2, Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7
Biochim. Biophys. Acta
1844
759-766
2014
2
-
2
-
7
-
-
-
-
-
4
-
-
9
-
-
2
-
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4
2
2
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-
7
2
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2
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2
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7
-
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4
-
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2
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4
2
2
-
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7
2
-
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-
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725162
Piattoni
A differential redox regulatio ...
Arabidopsis thaliana
Int. J. Mol. Sci.
14
8073-8092
2013
-
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3
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1
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2
1
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1
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1
2
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1
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3
2
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1
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1
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1
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741625
Centeno-Leija
Metabolic and transcriptional ...
Streptococcus mutans
Antonie van Leeuwenhoek
104
913-924
2013
-
-
1
-
1
-
-
-
-
-
-
1
-
1
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1
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1
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1
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2
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1
2
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1
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1
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-
1
-
1
-
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1
-
-
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1
1
-
-
-
741834
Arutyunov
An unusual effect of NADP+ on ...
Streptococcus mutans
Biochem. Cell Biol.
91
295-302
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
2
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1
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1
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1
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1
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-
-
-
-
1
-
-
1
-
-
-
-
-
-
2
2
-
-
-
724917
Avilan
-
Regulation of glyceraldehyde-3 ...
Asterionella formosa, Chlamydomonas reinhardtii, Pseudocharaciopsis ovalis
Eur. J. Phycol.
47
207-215
2012
3
-
-
-
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-
2
-
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3
-
3
-
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3
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3
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3
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3
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3
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2
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3
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3
-
-
-
-
-
3
-
-
-
3
-
-
3
-
-
725013
Ito
Comparative analysis of two gl ...
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7
FEBS Lett.
586
3097-3103
2012
3
-
1
-
2
-
1
15
-
-
3
4
-
7
-
-
1
-
-
-
-
-
8
2
2
-
-
-
2
-
-
2
-
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4
-
2
3
-
4
-
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1
-
18
-
-
6
4
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2
-
-
-
-
8
2
3
-
-
-
3
-
-
-
2
-
-
2
12
18
712646
Guo
Improving ethanol productivity ...
Bacillus cereus
J. Ind. Microbiol. Biotechnol.
38
935-943
2011
-
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1
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-
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1
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1
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1
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1
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1
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1
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1
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-
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-
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-
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724306
Erales
Molecular mechanism of NADPH-g ...
Chlamydomonas reinhardtii
Biochemistry
50
2881-2888
2011
-
-
1
-
4
-
1
3
-
-
-
1
-
1
-
-
1
-
-
-
-
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1
-
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1
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1
1
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4
-
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1
-
3
-
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1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
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-
-
-
-
-
-
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-
726000
Matsubara
Biochemical and genetic charac ...
Thermococcus kodakarensis
Mol. Microbiol.
81
1300-1312
2011
1
-
1
-
-
-
2
-
-
-
2
2
-
4
-
-
1
-
-
-
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2
1
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-
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-
-
2
1
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1
-
1
2
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2
1
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2
2
-
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-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
698095
Gao
Evolutionary and expression st ...
Oryza sativa
Gene
431
86-94
2009
-
-
1
-
-
-
-
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-
1
1
-
1
-
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1
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1
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1
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1
1
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1
1
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1
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1
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688646
Bustos
Involvement of non-phosphoryla ...
Triticum aestivum, Zea mays
J. Plant Physiol.
165
456-461
2008
2
-
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5
-
2
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6
2
-
2
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-
2
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2
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2
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2
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2
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5
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2
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2
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2
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2
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2
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-
699988
Martinez
Replacing Escherichia coli NAD ...
Clostridium acetobutylicum
Metab. Eng.
10
352-359
2008
-
-
-
-
1
-
-
-
-
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1
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1
1
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-
679719
Ettema
The non-phosphorylating glycer ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
Extremophiles
12
75-88
2007
5
-
1
-
-
-
4
6
-
-
3
1
-
13
-
-
1
-
-
-
1
-
17
2
-
-
-
-
-
-
-
2
-
-
-
5
-
4
7
-
-
-
-
12
-
14
-
-
11
1
-
-
-
3
-
-
1
-
17
3
-
-
-
-
-
-
-
-
-
-
-
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-
687467
Wu
PCR-mediated recombination of ...
Talipariti tiliaceum
J. Biochem. Mol. Biol.
40
172-179
2007
-
-
-
-
-
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1
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3
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1
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2
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671167
Kitatani
Structure of apo-glyceraldehyd ...
Synechococcus elongatus PCC 7942
Acta crystallogr. Sect. F
62
727-730
2006
-
-
1
1
-
-
-
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1
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1
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2
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1
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1
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1
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1
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1
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672132
DAmbrosio
The first crystal structure of ...
Streptococcus mutans
Biochemistry
45
2978-2986
2006
-
-
1
1
1
-
-
-
-
-
-
1
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1
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1
-
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1
1
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1
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1
1
1
1
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1
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1
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1
1
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673549
Lebreton
Mapping of the interaction sit ...
Chlamydomonas reinhardtii
FEBS J.
273
3358-3369
2006
-
-
-
-
-
-
1
-
1
-
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1
-
1
-
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1
-
-
-
-
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1
-
1
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1
1
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-
1
-
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-
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1
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1
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1
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1
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1
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1
-
1
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1
1
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-
-
-
-
-
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-
-
673728
Asanuma
Presence of NADP+-specific gly ...
Streptococcus equinus
FEMS Microbiol. Lett.
257
17-23
2006
1
-
1
-
-
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
11
-
2
-
3
-
-
-
2
-
-
1
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
11
-
2
-
3
-
-
-
2
-
-
1
-
-
-
-
-
-
675796
Harris
Enzymic analysis of NADPH meta ...
no activity in Penicillium chrysogenum
Metab. Eng.
8
91-101
2006
-
-
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1
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676550
Rius
Characterization of an Arabido ...
Arabidopsis thaliana
Plant Mol. Biol.
61
945-957
2006
-
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-
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-
-
-
-
2
-
-
1
-
4
-
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