BRENDA - Enzyme Database
show all sequences of 1.2.1.9

Purification of recombinant non-phosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase from Streptococcus pyogenes expressed in E. coli

Iddar, A.; Valverde, F.; Serrano, A.; Soukri, A.; Mol. Cell. Biochem. 247, 195-203 (2003)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression by functional complementation of the Escherichia coli gap mutant W3CG
Streptococcus pyogenes
Inhibitors
Inhibitors
Commentary
Organism
Structure
iodoacetamide
1 mM, 85% inhibition
Streptococcus pyogenes
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.385
-
NADP+
pH 8.5, 25°C
Streptococcus pyogenes
0.666
-
D-Glyceraldehyde-3-phosphate
pH 8.5, 25°C
Streptococcus pyogenes
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55000
-
4 * 55000, SDS-PAGE
Streptococcus pyogenes
224000
-
non-denaturing PAGE
Streptococcus pyogenes
Organism
Organism
UniProt
Commentary
Textmining
Streptococcus pyogenes
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant enzyme
Streptococcus pyogenes
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
143.2
-
-
Streptococcus pyogenes
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
D-glyceraldehyde-3-phosphate + NADP+
-
656781
Streptococcus pyogenes
D-3-phosphoglycerate + NADPH
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 55000, SDS-PAGE
Streptococcus pyogenes
Synonyms
Synonyms
Commentary
Organism
non-phosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase
-
Streptococcus pyogenes
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
-
Streptococcus pyogenes
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
10 min, inactivation above
Streptococcus pyogenes
75
-
10 min, complete inactivation
Streptococcus pyogenes
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Streptococcus pyogenes
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.5
12
pH 7.5: about 35% of maximal activity, pH 12.0: about 60% of maximal activity
Streptococcus pyogenes
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Streptococcus pyogenes
chromatofocusing
-
4.9
Cloned(Commentary) (protein specific)
Commentary
Organism
expression by functional complementation of the Escherichia coli gap mutant W3CG
Streptococcus pyogenes
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
iodoacetamide
1 mM, 85% inhibition
Streptococcus pyogenes
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.385
-
NADP+
pH 8.5, 25°C
Streptococcus pyogenes
0.666
-
D-Glyceraldehyde-3-phosphate
pH 8.5, 25°C
Streptococcus pyogenes
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55000
-
4 * 55000, SDS-PAGE
Streptococcus pyogenes
224000
-
non-denaturing PAGE
Streptococcus pyogenes
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme
Streptococcus pyogenes
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
143.2
-
-
Streptococcus pyogenes
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
D-glyceraldehyde-3-phosphate + NADP+
-
656781
Streptococcus pyogenes
D-3-phosphoglycerate + NADPH
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 55000, SDS-PAGE
Streptococcus pyogenes
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
-
Streptococcus pyogenes
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
10 min, inactivation above
Streptococcus pyogenes
75
-
10 min, complete inactivation
Streptococcus pyogenes
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Streptococcus pyogenes
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.5
12
pH 7.5: about 35% of maximal activity, pH 12.0: about 60% of maximal activity
Streptococcus pyogenes
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Streptococcus pyogenes
chromatofocusing
-
4.9
Other publictions for EC 1.2.1.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739907
Komati Reddy
Metabolic engineering of an AT ...
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Appl. Environ. Microbiol.
81
1996-2005
2015
-
-
-
-
-
-
-
-
-
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6
-
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-
-
-
-
-
1
1
-
-
-
724471
Ito
Engineering the allosteric pro ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2, Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7
Biochim. Biophys. Acta
1844
759-766
2014
2
-
2
-
7
-
-
-
-
-
4
-
-
9
-
-
2
-
-
-
-
-
4
2
6
2
-
-
7
2
-
-
-
-
-
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2
-
2
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-
7
-
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-
-
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4
-
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2
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4
2
2
-
-
7
2
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-
725162
Piattoni
A differential redox regulatio ...
Arabidopsis thaliana
Int. J. Mol. Sci.
14
8073-8092
2013
-
-
-
-
-
-
3
-
-
-
-
1
-
2
1
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1
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2
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1
2
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1
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3
2
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1
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1
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-
1
-
-
-
-
-
-
-
-
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-
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-
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-
741625
Centeno-Leija
Metabolic and transcriptional ...
Streptococcus mutans
Antonie van Leeuwenhoek
104
913-924
2013
-
-
1
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
4
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
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-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
741834
Arutyunov
An unusual effect of NADP+ on ...
Streptococcus mutans
Biochem. Cell Biol.
91
295-302
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
2
-
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
2
2
-
-
-
724917
Avilan
-
Regulation of glyceraldehyde-3 ...
Asterionella formosa, Chlamydomonas reinhardtii, Pseudocharaciopsis ovalis
Eur. J. Phycol.
47
207-215
2012
3
-
-
-
-
-
2
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
3
-
-
3
-
-
-
3
-
-
3
-
-
-
-
2
-
-
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-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
3
-
-
-
3
-
-
3
-
-
725013
Ito
Comparative analysis of two gl ...
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7
FEBS Lett.
586
3097-3103
2012
3
-
1
-
2
-
1
15
-
-
3
4
-
7
-
-
1
-
-
-
-
-
8
2
5
2
-
-
-
2
-
-
2
-
-
-
4
-
2
3
-
4
-
-
1
-
18
-
-
6
4
-
-
-
2
-
-
-
-
8
2
3
-
-
-
3
-
-
-
2
-
-
2
12
18
712646
Guo
Improving ethanol productivity ...
Bacillus cereus
J. Ind. Microbiol. Biotechnol.
38
935-943
2011
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
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-
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1
-
2
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1
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1
1
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1
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-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
724306
Erales
Molecular mechanism of NADPH-g ...
Chlamydomonas reinhardtii
Biochemistry
50
2881-2888
2011
-
-
1
-
4
-
1
3
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
4
-
-
1
-
3
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726000
Matsubara
Biochemical and genetic charac ...
Thermococcus kodakarensis
Mol. Microbiol.
81
1300-1312
2011
1
-
1
-
-
-
2
-
-
-
2
2
-
4
-
-
1
-
-
-
-
-
2
1
2
-
-
-
-
-
-
-
2
1
-
-
1
-
1
2
-
-
-
-
2
1
-
-
-
2
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
698095
Gao
Evolutionary and expression st ...
Oryza sativa
Gene
431
86-94
2009
-
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
1
-
-
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-
1
1
-
-
-
-
-
-
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-
1
1
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-
-
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1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688646
Bustos
Involvement of non-phosphoryla ...
Triticum aestivum, Zea mays
J. Plant Physiol.
165
456-461
2008
2
-
-
-
-
-
5
-
2
-
-
-
-
6
2
-
2
-
-
2
-
-
2
-
4
-
-
-
-
-
-
-
2
-
-
-
2
-
-
2
-
-
-
-
5
-
-
2
-
-
-
-
2
-
2
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
699988
Martinez
Replacing Escherichia coli NAD ...
Clostridium acetobutylicum
Metab. Eng.
10
352-359
2008
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
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-
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1
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-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
679719
Ettema
The non-phosphorylating glycer ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
Extremophiles
12
75-88
2007
5
-
1
-
-
-
4
6
-
-
3
1
-
13
-
-
1
-
-
-
1
-
17
2
9
-
-
-
-
-
-
-
2
-
-
-
5
-
4
7
-
-
-
-
12
-
14
-
-
11
1
-
-
-
3
-
-
1
-
17
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687467
Wu
PCR-mediated recombination of ...
Talipariti tiliaceum
J. Biochem. Mol. Biol.
40
172-179
2007
-
-
-
-
-
-
-
-
1
-
-
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-
3
-
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1
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2
-
2
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1
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1
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1
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1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671167
Kitatani
Structure of apo-glyceraldehyd ...
Synechococcus elongatus PCC 7942 = FACHB-805
Acta crystallogr. Sect. F
62
727-730
2006
-
-
1
1
-
-
-
-
-
1
-
1
-
2
-
-
1
-
-
-
-
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1
-
3
-
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-
-
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-
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2
-
-
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1
2
1
-
-
-
-
-
-
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1
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1
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1
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1
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-
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-
-
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-
-
-
-
-
-
-
-
672132
DAmbrosio
The first crystal structure of ...
Streptococcus mutans
Biochemistry
45
2978-2986
2006
-
-
1
1
1
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
1
3
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673549
Lebreton
Mapping of the interaction sit ...
Chlamydomonas reinhardtii
FEBS J.
273
3358-3369
2006
-
-
-
-
-
-
1
-
1
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
3
1
-
-
1
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
1
-
-
-
1
-
-
-
-
1
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
673728
Asanuma
Presence of NADP+-specific gly ...
Streptococcus equinus
FEMS Microbiol. Lett.
257
17-23
2006
1
-
1
-
-
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
11
-
2
-
3
3
-
-
-
2
-
-
1
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
11
-
2
-
3
-
-
-
2
-
-
1
-
-
-
-
-
-
675796
Harris
Enzymic analysis of NADPH meta ...
no activity in Penicillium chrysogenum
Metab. Eng.
8
91-101
2006
-
-
-
-
-
-
-
-
-
-
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1
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1
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-
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676550
Rius
Characterization of an Arabido ...
Arabidopsis thaliana
Plant Mol. Biol.
61
945-957
2006
-
-
-
-
-
-
-
-
2
-
-
1
-
5
-
-
-
-
-
-
2
-
1
-
3
1
-
-
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1
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