BRENDA - Enzyme Database show
show all sequences of 1.2.1.9

Expression, purification, and characterization of recombinant nonphosphorylating NADP-dependent glyceraldehyde-3-phosphate dehydrogenase from Clostridium acetobutylicum

Iddar, A.; Valverde, F.; Serrano, A.; Soukri, A.; Protein Expr. Purif. 25, 519-526 (2002)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli BL-21
Clostridium acetobutylicum
Inhibitors
Inhibitors
Commentary
Organism
Structure
iodoacetamide
1 mM, 76% inhibition
Clostridium acetobutylicum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
NADP+
pH 8.5, 25°C
Clostridium acetobutylicum
0.545
-
D-Glyceraldehyde-3-phosphate
pH 8.5, 25°C
Clostridium acetobutylicum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
4 * 50000, SDS-PAGE
Clostridium acetobutylicum
204000
-
non-denaturing PAGE
Clostridium acetobutylicum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clostridium acetobutylicum
-
ATCC 824
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme
Clostridium acetobutylicum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
75
-
-
Clostridium acetobutylicum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glyceraldehyde-3-phosphate + NADP+
-
657250
Clostridium acetobutylicum
D-3-phosphoglycerate + NADPH
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 50000, SDS-PAGE
Clostridium acetobutylicum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
65
-
-
Clostridium acetobutylicum
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
-
10 min, inactivation above
Clostridium acetobutylicum
95
-
10 min, complete inactivation
Clostridium acetobutylicum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.2
-
-
Clostridium acetobutylicum
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
absolute specificity
Clostridium acetobutylicum
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Clostridium acetobutylicum
chromatofocusing
-
4.2
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli BL-21
Clostridium acetobutylicum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
absolute specificity
Clostridium acetobutylicum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
iodoacetamide
1 mM, 76% inhibition
Clostridium acetobutylicum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
NADP+
pH 8.5, 25°C
Clostridium acetobutylicum
0.545
-
D-Glyceraldehyde-3-phosphate
pH 8.5, 25°C
Clostridium acetobutylicum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50000
-
4 * 50000, SDS-PAGE
Clostridium acetobutylicum
204000
-
non-denaturing PAGE
Clostridium acetobutylicum
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme
Clostridium acetobutylicum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
75
-
-
Clostridium acetobutylicum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glyceraldehyde-3-phosphate + NADP+
-
657250
Clostridium acetobutylicum
D-3-phosphoglycerate + NADPH
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 50000, SDS-PAGE
Clostridium acetobutylicum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
65
-
-
Clostridium acetobutylicum
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
-
10 min, inactivation above
Clostridium acetobutylicum
95
-
10 min, complete inactivation
Clostridium acetobutylicum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.2
-
-
Clostridium acetobutylicum
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Clostridium acetobutylicum
chromatofocusing
-
4.2
Other publictions for EC 1.2.1.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739907
Komati Reddy
Metabolic engineering of an AT ...
Clostridium acetobutylicum
Appl. Environ. Microbiol.
81
1996-2005
2015
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-
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-
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-
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6
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1
1
-
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-
724471
Ito
Engineering the allosteric pro ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2, Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7
Biochim. Biophys. Acta
1844
759-766
2014
2
-
2
-
7
-
-
-
-
-
4
-
-
9
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2
-
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4
2
2
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7
2
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2
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2
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7
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4
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2
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4
2
2
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7
2
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725162
Piattoni
A differential redox regulatio ...
Arabidopsis thaliana
Int. J. Mol. Sci.
14
8073-8092
2013
-
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-
3
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1
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2
1
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1
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1
2
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1
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3
2
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1
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1
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1
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741625
Centeno-Leija
Metabolic and transcriptional ...
Streptococcus mutans
Antonie van Leeuwenhoek
104
913-924
2013
-
-
1
-
1
-
-
-
-
-
-
1
-
1
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1
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1
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1
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2
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1
2
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1
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1
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1
-
1
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1
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1
1
-
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-
741834
Arutyunov
An unusual effect of NADP+ on ...
Streptococcus mutans
Biochem. Cell Biol.
91
295-302
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
2
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1
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1
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1
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1
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-
-
1
-
-
1
-
-
-
-
-
-
2
2
-
-
-
724917
Avilan
-
Regulation of glyceraldehyde-3 ...
Asterionella formosa, Chlamydomonas reinhardtii, Pseudocharaciopsis ovalis
Eur. J. Phycol.
47
207-215
2012
3
-
-
-
-
-
2
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-
3
-
3
-
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3
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3
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3
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3
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3
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2
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3
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3
-
-
-
-
-
3
-
-
-
3
-
-
3
-
-
725013
Ito
Comparative analysis of two gl ...
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7
FEBS Lett.
586
3097-3103
2012
3
-
1
-
2
-
1
15
-
-
3
4
-
7
-
-
1
-
-
-
-
-
8
2
2
-
-
-
2
-
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2
-
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4
-
2
3
-
4
-
-
1
-
18
-
-
6
4
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-
-
2
-
-
-
-
8
2
3
-
-
-
3
-
-
-
2
-
-
2
12
18
712646
Guo
Improving ethanol productivity ...
Bacillus cereus
J. Ind. Microbiol. Biotechnol.
38
935-943
2011
-
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1
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-
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-
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1
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1
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1
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1
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1
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1
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1
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724306
Erales
Molecular mechanism of NADPH-g ...
Chlamydomonas reinhardtii
Biochemistry
50
2881-2888
2011
-
-
1
-
4
-
1
3
-
-
-
1
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1
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1
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1
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1
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1
1
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4
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1
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3
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1
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1
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1
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-
726000
Matsubara
Biochemical and genetic charac ...
Thermococcus kodakarensis
Mol. Microbiol.
81
1300-1312
2011
1
-
1
-
-
-
2
-
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-
2
2
-
4
-
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1
-
-
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2
1
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2
1
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1
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1
2
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2
1
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2
2
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1
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2
1
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-
-
-
-
-
2
2
-
-
-
698095
Gao
Evolutionary and expression st ...
Oryza sativa
Gene
431
86-94
2009
-
-
1
-
-
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-
1
1
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1
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1
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1
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1
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1
1
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1
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1
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1
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688646
Bustos
Involvement of non-phosphoryla ...
Triticum aestivum, Zea mays
J. Plant Physiol.
165
456-461
2008
2
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5
-
2
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6
2
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2
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2
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2
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2
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2
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2
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5
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2
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2
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2
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2
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2
-
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-
699988
Martinez
Replacing Escherichia coli NAD ...
Clostridium acetobutylicum
Metab. Eng.
10
352-359
2008
-
-
-
-
1
-
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1
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1
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-
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-
1
1
-
-
-
679719
Ettema
The non-phosphorylating glycer ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
Extremophiles
12
75-88
2007
5
-
1
-
-
-
4
6
-
-
3
1
-
13
-
-
1
-
-
-
1
-
17
2
-
-
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-
-
-
-
2
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5
-
4
7
-
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12
-
14
-
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11
1
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-
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3
-
-
1
-
17
3
-
-
-
-
-
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-
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687467
Wu
PCR-mediated recombination of ...
Talipariti tiliaceum
J. Biochem. Mol. Biol.
40
172-179
2007
-
-
-
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1
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3
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1
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2
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671167
Kitatani
Structure of apo-glyceraldehyd ...
Synechococcus elongatus PCC 7942
Acta crystallogr. Sect. F
62
727-730
2006
-
-
1
1
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1
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1
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2
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1
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672132
DAmbrosio
The first crystal structure of ...
Streptococcus mutans
Biochemistry
45
2978-2986
2006
-
-
1
1
1
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1
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1
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1
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1
1
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1
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1
1
1
1
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1
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1
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1
1
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673549
Lebreton
Mapping of the interaction sit ...
Chlamydomonas reinhardtii
FEBS J.
273
3358-3369
2006
-
-
-
-
-
-
1
-
1
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1
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1
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1
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1
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1
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1
1
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1
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1
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1
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1
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1
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1
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1
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1
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1
1
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-
-
-
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-
-
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-
673728
Asanuma
Presence of NADP+-specific gly ...
Streptococcus equinus
FEMS Microbiol. Lett.
257
17-23
2006
1
-
1
-
-
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
11
-
2
-
3
-
-
-
2
-
-
1
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
11
-
2
-
3
-
-
-
2
-
-
1
-
-
-
-
-
-
675796
Harris
Enzymic analysis of NADPH meta ...
no activity in Penicillium chrysogenum
Metab. Eng.
8
91-101
2006
-
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1
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676550
Rius
Characterization of an Arabido ...
Arabidopsis thaliana
Plant Mol. Biol.
61
945-957
2006
-
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-
-
2
-
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1
-
5
-
-
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-
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2
-
1
-
1
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1
-
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1
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1
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-
-
2
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
656283
Rahuel-Clermont
Thermal destabilization of non ...
Streptococcus mutans
J. Biol. Chem.
280
18590-18597
2005
-
-
-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
1
-
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-
-
1
-
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-
-
-
1
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
674077
Iddar
Widespread occurrence of non-p ...
Bacillus cereus, Bacillus licheniformis, Bacillus sp. (in: Bacteria), Bacillus thuringiensis, Clostridioides difficile, Clostridium acetobutylicum, Clostridium pasteurianum, Clostridium perfringens, Clostridium sporogenes, Streptococcus agalactiae, Streptococcus pyogenes, Streptococcus sp.
Int. Microbiol.
8
251-258
2005
-
-
-
-
-
-
-
-
-
-
10
12
-
18
-
-
12
-
-
-
12
-
12
-
12
-
-
-
12
-
-
12
-
-
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-
12
-
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-
-
-
-
-
-
10
12
-
-
-
12
-
-
12
-
12
-
12
-
-
-
12
-
-
-
-
-
-
-
-
-
676575
Sparla
Regulation of photosynthetic G ...
Spinacia oleracea
Plant Physiol.
138
2210-2219
2005
-
-
1
-
5
-
-
20
1
-
12
1
-
1
-
-
1
-
-
1
-
-
1
-
1
-
-
-
1
-
-
1
-
-
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-
1
1
-
5
-
-
-
-
20
1
-
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1
-
-
-
1
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
654427
Arutyunov
The activation of glycolysis p ...
Streptococcus mutans
Biochem. Biophys. Res. Commun.
300
149-154
2003
-
-
-
-
-
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656781
Iddar
Purification of recombinant no ...
Streptococcus pyogenes
Mol. Cell. Biochem.
247
195-203
2003
-
-
1
-
-
-
1
2
-
-
2
-
-
4
-
-
1
-
-
-
1
-
1
1
1
-
2
-
1
1
-
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1
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-
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-
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2
-
-
-
-
1
-
-
1
-
1
1
1
-
2
-
1
1
-
1
-
-
-
-
-
-
657058
Bustos
Phosphorylated non-phosphoryla ...
Triticum aestivum
Plant Physiol.
133
2081-2088
2003
-
-
-
-
-
-
2
-
1
1
-
-
-
4
-
-
1
-
-
3
-
-
1
-
-
-
-
-
-
-
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1
-
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-
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-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655561
Bustos
Non-phosphorylating glyceralde ...
Triticum aestivum
FEBS Lett.
530
169-173
2002
-
-
-
-
-
-
-
5
1
-
1
-
-
3
-
1
1
-
-
5
-
-
1
-
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-
-
-
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1
-
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1
-
-
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-
5
1
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-
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-
1
1
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656082
Marchal
Characterization of the amino ...
Streptococcus mutans
J. Biol. Chem.
277
39235-39242
2002
-
-
-
-
4
-
1
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
5
-
-
-
1
2
-
-
-
-
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1
-
4
-
-
1
2
3
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
657096
Iglesias
-
On the interaction of substrat ...
Apium graveolens
Plant Sci.
162
689-696
2002
-
-
-
-
-
-
8
5
-
-
-
-
-
1
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
5
9
-
-
-
-
-
5
-
-
-
-
8
9
5
-
-
-
-
-
-
-
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
657250
Iddar
Expression, purification, and ...
Clostridium acetobutylicum
Protein Expr. Purif.
25
519-526
2002
-
-
1
-
-
-
1
2
-
-
2
-
-
4
-
-
1
-
-
-
1
-
1
1
1
-
2
-
1
-
-
1
-
1
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1
1
-
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1
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2
-
-
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-
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-
-
1
-
1
1
1
-
2
-
1
-
-
1
-
-
-
-
-
-
288346
Marchal
Engineered nonphosphorylating ...
Streptococcus mutans
Eur. J. Biochem.
268
5764-5770
2001
-
-
-
-
2
-
2
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
1
-
1
6
-
-
-
-
-
1
-
2
-
-
2
6
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
288348
Nakamura
Crystallization and preliminar ...
Synechococcus sp.
Acta Crystallogr. Sect. D
57
879-881
2001
1
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
1
1
-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
288347
Charron
-
Crystallization and preliminar ...
Methanothermus fervidus
Acta Crystallogr. Sect. D
55
1253-1255
1999
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
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-
-
1
-
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-
-
-
1
1
-
-
-
-
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-
-
-
-
-
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-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
288345
Tamoi
Enzymic and molecular characte ...
Synechococcus sp.
Biochem. J.
316
685-690
1996
-
-
1
-
-
-
-
2
-
-
2
-
-
1
1
-
1
1
-
-
2
-
1
1
-
-
-
-
-
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
2
-
-
2
-
-
1
-
1
-
-
2
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288337
Iglesias
Purification and kinetic and s ...
Spinacia oleracea
Arch. Biochem. Biophys.
260
830-840
1988
-
-
-
-
-
-
4
2
-
-
1
1
-
2
-
-
1
1
-
2
1
1
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
2
-
-
1
1
-
-
-
1
-
2
1
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288338
Iglesias
-
Purification and properties of ...
Chlamydomonas reinhardtii
Biochim. Biophys. Acta
925
1-10
1987
-
-
1
-
-
-
6
2
-
-
1
1
-
1
-
-
1
-
-
-
1
1
2
1
-
-
1
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
6
-
2
-
-
1
1
-
-
-
1
-
-
1
1
2
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
94715
Rumpho
A pathway for photosynthetic c ...
Apium graveolens
Plant Physiol.
73
869-873
1983
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288339
Pupillo
Subunit structure of three gly ...
Arum italicum, Beta vulgaris, Ficaria verna, Spinacia oleracea
Arch. Biochem. Biophys.
194
581-592
1979
-
-
-
-
-
-
-
-
1
-
3
-
-
4
-
-
1
-
-
2
1
-
4
1
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
-
1
-
3
-
-
-
-
1
-
2
1
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288343
Crow
Separation and properties of N ...
Streptococcus mutans
J. Biol. Chem.
254
1134-1142
1979
-
-
-
-
-
-
4
-
-
3
1
1
-
1
-
-
1
-
-
-
1
1
2
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
3
1
1
-
-
-
1
-
-
1
1
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
288344
Mann
-
Inhibition of spinach glyceral ...
Spinacia oleracea
Nature
282
535-536
1979
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288340
Kelly
Nonreversible D-glyceraldehyde ...
Arachis hypogaea, Pisum sativum, Ricinus communis, Zea mays
Plant Physiol.
52
111-118
1973
-
-
-
-
-
-
11
6
-
-
-
4
-
4
-
-
3
-
-
4
1
-
4
-
-
-
-
-
1
1
-
4
-
-
-
-
-
-
4
-
-
-
-
11
-
6
-
-
-
4
-
-
-
3
-
4
1
-
4
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
288341
Jacob
Glyceraldehyde-3-phosphate deh ...
Hevea brasiliensis
Eur. J. Biochem.
31
255-265
1972
3
-
-
-
-
-
5
2
1
-
1
1
-
1
-
-
1
-
-
1
1
-
1
1
-
-
1
-
1
-
-
2
-
-
-
3
-
-
2
-
-
-
-
5
-
2
1
-
1
1
-
-
-
1
-
1
1
-
1
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-