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Literature summary for 1.2.1.9 extracted from

  • Bustos, D.M.; Bustamante, C.A.; Iglesias, A.A.
    Involvement of non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase in response to oxidative stress (2008), J. Plant Physiol., 165, 456-461.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
methyl viologen the specific activity of the enzyme increases up to 2fold after oxidative conditions imposed by methylviologen, expression of NP-Ga3PDHase in plants is induced by high treatments with methyl viologen Triticum aestivum
methyl viologen the specific activity of the enzyme increases up to 2fold after oxidative conditions imposed by methylviologen, expression of NP-Ga3PDHase in plants is induced by high treatments with methyl viologen Zea mays

Inhibitors

Inhibitors Comment Organism Structure
Diamide sensitive to inactivation by diamide, inactivation is protected by D-glyceraldehyde 3-phosphate (with no effect by NADP+) and it is completely reversed by 2-mercaptoethanol Triticum aestivum
H2O2 72% residual activity at 1 mM H2O2 Triticum aestivum
H2O2 72% residual activity at 1 mM H2O2 Zea mays
Mg2+ near 75% of the activity is lost when 5 mM Mg2+ is included in the incubation medium Triticum aestivum
Mg2+ near 75% of the activity is lost when 5 mM Mg2+ is included in the incubation medium Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Triticum aestivum 5829
-
cytosol
-
Zea mays 5829
-

Organism

Organism UniProt Comment Textmining
Triticum aestivum
-
-
-
Zea mays
-
-
-

Oxidation Stability

Oxidation Stability Organism
a relative stability of NP-Ga3PDHase mainly accounts for the increase in specific activity observed in treatments with low and medium levels (up to 0.01 mM) of methyl viologen Triticum aestivum
a relative stability of NP-Ga3PDHase mainly accounts for the increase in specific activity observed in treatments with low and medium levels (up to 0.01 mM) of methyl viologen Zea mays

Purification (Commentary)

Purification (Comment) Organism
partial purification Triticum aestivum
partial purification Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Triticum aestivum
-
seedling
-
Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate + NADP+ + H2O
-
Triticum aestivum 3-phospho-D-glycerate + NADPH + H+
-
ir
D-glyceraldehyde 3-phosphate + NADP+ + H2O
-
Zea mays 3-phospho-D-glycerate + NADPH + H+
-
ir

Synonyms

Synonyms Comment Organism
non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase
-
Triticum aestivum
non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase
-
Zea mays
NP-Ga3PDHase
-
Triticum aestivum
NP-Ga3PDHase
-
Zea mays

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Triticum aestivum
NADP+
-
Zea mays