BRENDA - Enzyme Database show
show all sequences of 1.2.1.9

Metabolic and transcriptional response of Escherichia coli with a NADP+-dependent glyceraldehyde 3-phosphate dehydrogenase from Streptococcus mutans

Centeno-Leija, S.; Utrilla, J.; Flores, N.; Rodriguez, A.; Gosset, G.; Martinez, A.; Antonie van Leeuwenhoek 104, 913-924 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene gapN, construction of the mutant Escherichia coli strain MG1655DELTAgapA::gapN using the fusion of the gapN gene with a chloramphenicol resistance cassette resulting in plasmid pTrcgapN
Streptococcus mutans
Engineering
Amino acid exchange
Commentary
Organism
additional information
the gapA gene, UniProt ID P0A9B2, EC 1.2.1.12, from Escherichia coli strain MG1655 is replaced by the gene gapN from Streptococcus mutans. The specific NADP+-GAPDH activity of the strain MG1655DgapA::gapN is 4.6times lower relative to strain MG1655DELTAgapA::gapN/pTrcgapN and no NAD+-GAPDH activity is detected. The specific NADP+-GAPDH activity levels in the derivative strain reveal that growth rate and glucose uptake differences are attributable to gapN expression level. The NADH/NAD+ ratio in the strain MG1655DELTAgapA::gapN/pTrcgapN decreases by 25% as compared to wild-type strain. In contrast, the NADPH/NADP+ ratio increases 2times indicating that the alteration in the turnover of NAD(P)H via glyceraldehyde 3-phosphate oxidation affects the redox levels of the strain MG1655DELTAgapA::gapN/pTrcgapN, which increases 2.8times the NADPH/NADH ratio
Streptococcus mutans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-glyceraldehyde 3-phosphate + NADP+ + H2O
Streptococcus mutans
-
3-phospho-D-glycerate + NADPH + 2 H+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptococcus mutans
Q59931
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glyceraldehyde 3-phosphate + NADP+ + H2O
-
741625
Streptococcus mutans
3-phospho-D-glycerate + NADPH + 2 H+
-
-
-
r
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Streptococcus mutans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
assay at
Streptococcus mutans
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Streptococcus mutans
NADPH
-
Streptococcus mutans
Cloned(Commentary) (protein specific)
Commentary
Organism
gene gapN, construction of the mutant Escherichia coli strain MG1655DELTAgapA::gapN using the fusion of the gapN gene with a chloramphenicol resistance cassette resulting in plasmid pTrcgapN
Streptococcus mutans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Streptococcus mutans
NADPH
-
Streptococcus mutans
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
the gapA gene, UniProt ID P0A9B2, EC 1.2.1.12, from Escherichia coli strain MG1655 is replaced by the gene gapN from Streptococcus mutans. The specific NADP+-GAPDH activity of the strain MG1655DgapA::gapN is 4.6times lower relative to strain MG1655DELTAgapA::gapN/pTrcgapN and no NAD+-GAPDH activity is detected. The specific NADP+-GAPDH activity levels in the derivative strain reveal that growth rate and glucose uptake differences are attributable to gapN expression level. The NADH/NAD+ ratio in the strain MG1655DELTAgapA::gapN/pTrcgapN decreases by 25% as compared to wild-type strain. In contrast, the NADPH/NADP+ ratio increases 2times indicating that the alteration in the turnover of NAD(P)H via glyceraldehyde 3-phosphate oxidation affects the redox levels of the strain MG1655DELTAgapA::gapN/pTrcgapN, which increases 2.8times the NADPH/NADH ratio
Streptococcus mutans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-glyceraldehyde 3-phosphate + NADP+ + H2O
Streptococcus mutans
-
3-phospho-D-glycerate + NADPH + 2 H+
-
-
r
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-glyceraldehyde 3-phosphate + NADP+ + H2O
-
741625
Streptococcus mutans
3-phospho-D-glycerate + NADPH + 2 H+
-
-
-
r
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Streptococcus mutans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
assay at
Streptococcus mutans
General Information
General Information
Commentary
Organism
metabolism
the EMP pathway can be controlled through the glyceraldehyde 3-phosphate node by NAD+-GAPDH activity, recombinant NADP+-GAPDH heterologous activity can also exert a similar response, which modulates the glucose uptake and also the acetic acid production rate
Streptococcus mutans
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the EMP pathway can be controlled through the glyceraldehyde 3-phosphate node by NAD+-GAPDH activity, recombinant NADP+-GAPDH heterologous activity can also exert a similar response, which modulates the glucose uptake and also the acetic acid production rate
Streptococcus mutans
Other publictions for EC 1.2.1.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739907
Komati Reddy
Metabolic engineering of an AT ...
Clostridium acetobutylicum
Appl. Environ. Microbiol.
81
1996-2005
2015
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6
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1
1
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724471
Ito
Engineering the allosteric pro ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2, Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7
Biochim. Biophys. Acta
1844
759-766
2014
2
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2
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7
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4
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9
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2
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4
2
2
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7
2
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2
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2
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7
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4
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4
2
2
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7
2
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725162
Piattoni
A differential redox regulatio ...
Arabidopsis thaliana
Int. J. Mol. Sci.
14
8073-8092
2013
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3
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1
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741625
Centeno-Leija
Metabolic and transcriptional ...
Streptococcus mutans
Antonie van Leeuwenhoek
104
913-924
2013
-
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1
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1
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1
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1
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1
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1
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1
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1
1
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741834
Arutyunov
An unusual effect of NADP+ on ...
Streptococcus mutans
Biochem. Cell Biol.
91
295-302
2013
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2
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1
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1
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2
2
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724917
Avilan
-
Regulation of glyceraldehyde-3 ...
Asterionella formosa, Chlamydomonas reinhardtii, Pseudocharaciopsis ovalis
Eur. J. Phycol.
47
207-215
2012
3
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2
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3
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3
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3
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3
-
-
725013
Ito
Comparative analysis of two gl ...
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7
FEBS Lett.
586
3097-3103
2012
3
-
1
-
2
-
1
15
-
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3
4
-
7
-
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1
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8
2
2
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2
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2
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4
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2
3
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4
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1
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18
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6
4
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2
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8
2
3
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3
-
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2
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2
12
18
712646
Guo
Improving ethanol productivity ...
Bacillus cereus
J. Ind. Microbiol. Biotechnol.
38
935-943
2011
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1
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1
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724306
Erales
Molecular mechanism of NADPH-g ...
Chlamydomonas reinhardtii
Biochemistry
50
2881-2888
2011
-
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1
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4
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1
3
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1
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1
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1
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726000
Matsubara
Biochemical and genetic charac ...
Thermococcus kodakarensis
Mol. Microbiol.
81
1300-1312
2011
1
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1
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2
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2
2
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4
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1
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1
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1
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2
2
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698095
Gao
Evolutionary and expression st ...
Oryza sativa
Gene
431
86-94
2009
-
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1
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1
1
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1
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688646
Bustos
Involvement of non-phosphoryla ...
Triticum aestivum, Zea mays
J. Plant Physiol.
165
456-461
2008
2
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5
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2
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6
2
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2
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2
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2
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2
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699988
Martinez
Replacing Escherichia coli NAD ...
Clostridium acetobutylicum
Metab. Eng.
10
352-359
2008
-
-
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1
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1
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1
1
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679719
Ettema
The non-phosphorylating glycer ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
Extremophiles
12
75-88
2007
5
-
1
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4
6
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3
1
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13
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1
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1
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17
2
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4
7
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12
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1
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1
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17
3
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687467
Wu
PCR-mediated recombination of ...
Talipariti tiliaceum
J. Biochem. Mol. Biol.
40
172-179
2007
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3
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671167
Kitatani
Structure of apo-glyceraldehyd ...
Synechococcus elongatus PCC 7942
Acta crystallogr. Sect. F
62
727-730
2006
-
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1
1
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672132
DAmbrosio
The first crystal structure of ...
Streptococcus mutans
Biochemistry
45
2978-2986
2006
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1
1
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1
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673549
Lebreton
Mapping of the interaction sit ...
Chlamydomonas reinhardtii
FEBS J.
273
3358-3369
2006
-
-
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1
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1
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1
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673728
Asanuma
Presence of NADP+-specific gly ...
Streptococcus equinus
FEMS Microbiol. Lett.
257
17-23
2006
1
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1
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1
2
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1
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11
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2
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3
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2
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1
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1
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11
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2
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1
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675796
Harris
Enzymic analysis of NADPH meta ...
no activity in Penicillium chrysogenum
Metab. Eng.
8
91-101
2006
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676550
Rius
Characterization of an Arabido ...
Arabidopsis thaliana
Plant Mol. Biol.
61
945-957
2006
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2
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1
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5
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2
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1
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1
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1
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