BRENDA - Enzyme Database
show all sequences of 1.2.1.90

Role of two different glyceraldehyde-3-phosphate dehydrogenases in controlling the reversible Embden-Meyerhof-Parnas pathway in Thermoproteus tenax: regulation on protein and transcript level

Brunner, N.A.; Siebers, B.; Hensel R.; Extremophiles 5, 101-109 (2001)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
D-glyceraldehyde 3-phosphate
pH 7.0, 70°C
Thermoproteus tenax
0.02
-
D-glyceraldehyde 3-phosphate
pH 7.0, 45°C
Thermoproteus tenax
1
-
NAD+
pH 7.0, 45°C
Thermoproteus tenax
3.1
-
NAD+
pH 7.0, 70°C
Thermoproteus tenax
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
D-glyceraldehyde 3-phosphate + NAD+ + H2O
Thermoproteus tenax
the enzyme is part of the modified Embden–Meyerhof–Parnas pathway, the main route for carbohydrate metabolism in Thermoproteus tenax
3-phospho-D-glycerate + NADH + 2 H+
-
-
ir
Organism
Organism
UniProt
Commentary
Textmining
Thermoproteus tenax
O57693
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:autotrophically-grown cell
specific activity of remains constant in autotrophically and heterotrophically grown cells
Thermoproteus tenax
-
culture condition:heterotrophically-grown cell
specific activity of remains constant in autotrophically and heterotrophically grown cells
Thermoproteus tenax
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
D-glyceraldehyde 3-phosphate + NAD+ + H2O
-
727454
Thermoproteus tenax
3-phospho-D-glycerate + NADH + 2 H+
-
-
-
ir
D-glyceraldehyde 3-phosphate + NAD+ + H2O
the enzyme is part of the modified Embden–Meyerhof–Parnas pathway, the main route for carbohydrate metabolism in Thermoproteus tenax
727454
Thermoproteus tenax
3-phospho-D-glycerate + NADH + 2 H+
-
-
-
ir
Synonyms
Synonyms
Commentary
Organism
GAPN
-
Thermoproteus tenax
nonphosphorylating NAD+-dependent GAPN
-
Thermoproteus tenax
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
D-glyceraldehyde 3-phosphate
pH 7.0, 70°C
Thermoproteus tenax
0.02
-
D-glyceraldehyde 3-phosphate
pH 7.0, 45°C
Thermoproteus tenax
1
-
NAD+
pH 7.0, 45°C
Thermoproteus tenax
3.1
-
NAD+
pH 7.0, 70°C
Thermoproteus tenax
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
D-glyceraldehyde 3-phosphate + NAD+ + H2O
Thermoproteus tenax
the enzyme is part of the modified Embden–Meyerhof–Parnas pathway, the main route for carbohydrate metabolism in Thermoproteus tenax
3-phospho-D-glycerate + NADH + 2 H+
-
-
ir
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:autotrophically-grown cell
specific activity of remains constant in autotrophically and heterotrophically grown cells
Thermoproteus tenax
-
culture condition:heterotrophically-grown cell
specific activity of remains constant in autotrophically and heterotrophically grown cells
Thermoproteus tenax
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
D-glyceraldehyde 3-phosphate + NAD+ + H2O
-
727454
Thermoproteus tenax
3-phospho-D-glycerate + NADH + 2 H+
-
-
-
ir
D-glyceraldehyde 3-phosphate + NAD+ + H2O
the enzyme is part of the modified Embden–Meyerhof–Parnas pathway, the main route for carbohydrate metabolism in Thermoproteus tenax
727454
Thermoproteus tenax
3-phospho-D-glycerate + NADH + 2 H+
-
-
-
ir
General Information
General Information
Commentary
Organism
metabolism
the enzyme is part of the modified Embden–Meyerhof–Parnas pathway, the main route for carbohydrate metabolism in Thermoproteus tenax
Thermoproteus tenax
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is part of the modified Embden–Meyerhof–Parnas pathway, the main route for carbohydrate metabolism in Thermoproteus tenax
Thermoproteus tenax
Other publictions for EC 1.2.1.90
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728132
Lorentzen
Structural basis of allosteric ...
Thermoproteus tenax
J. Mol. Biol.
341
815-828
2004
4
-
1
1
-
-
1
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
3
-
2
1
-
-
-
1
-
-
2
-
-
-
4
-
1
2
1
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
288349
Pohl
The crystal structure of the a ...
Thermoproteus tenax
J. Biol. Chem.
277
19938-19945
2002
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727454
Brunner
Role of two different glyceral ...
Thermoproteus tenax
Extremophiles
5
101-109
2001
-
-
-
-
-
-
-
4
-
-
-
1
-
1
-
-
-
-
-
2
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
727825
Brunner
NAD+-dependent glyceraldehyde- ...
Thermoproteus tenax
J. Biol. Chem.
273
6149-6156
1998
7
-
1
-
-
-
5
3
-
1
2
1
-
1
-
-
1
-
-
-
-
-
2
1
1
1
-
1
-
1
-
-
1
1
-
-
7
-
1
1
-
-
-
-
5
1
3
-
1
2
1
-
-
-
1
-
-
-
-
2
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-