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Literature summary for 1.2.1.92 extracted from

  • Pathiraja, D.; Kim, K.H.; Choi, I.G.
    Rapid and robust enzymatic sensing and quantitation of 3,6-anhydro-L-galactose in a heterogeneous sugar mixture (2017), Carbohydr. Res., 446-447, 13-18 .
    View publication on PubMed
Search for more literature for 1.2.1.92

Activating Compound

Activating Compound Comment Organism Structure
EDTA 132.2% activity at 1 mM Vibrio sp. EJY3

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 80.8% residual activity at 1 mM Vibrio sp. EJY3
Cd2+ complete inhibition at 1 mM Vibrio sp. EJY3
Co2+ complete inhibition at 1 mM Vibrio sp. EJY3
Cu2+ complete inhibition at 1 mM Vibrio sp. EJY3
Mn2+ 68.2% residual activity at 1 mM Vibrio sp. EJY3
additional information not inhibited by EDTA Vibrio sp. EJY3
Ni2+ complete inhibition at 1 mM Vibrio sp. EJY3
Zn2+ complete inhibition at 1 mM Vibrio sp. EJY3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
 3,6-anhydro-alpha-L-galactopyranose at pH 8.0 and 20°C Vibrio sp. EJY3

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mg2+, Na+ and K+ have no effect on the enzyme activity Vibrio sp. EJY3

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3,6-anhydro-alpha-L-galactopyranose + NADP+ + H2O Vibrio sp. EJY3 the enzyme is highly specific to 3,6-anhydro-alpha-L-galactopyranose 3,6-anhydro-L-galactonate + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Vibrio sp. EJY3 H2IFE7
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Vibrio sp. EJY3

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,6-anhydro-alpha-L-galactopyranose + NADP+ + H2O the enzyme is highly specific to 3,6-anhydro-alpha-L-galactopyranose Vibrio sp. EJY3 3,6-anhydro-L-galactonate + NADPH + H+
-
 ?
additional information no activity with D-glucose, D-galactose, D-fructose, sucrose, maltose, arabinose, xylose, agarohexose, agarotetrose and neoagarobiose Vibrio sp. EJY3 ?
-
-

Synonyms

Synonyms Comment Organism
AHGD
-
 Vibrio sp. EJY3
anhydrogalactose dehydrogenase
-
 Vibrio sp. EJY3

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
-
 Vibrio sp. EJY3

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 50 the enzyme shows 50% activity after 12 h at 20°C and pH 8.0. At 50°C, the half-life of the enzyme is less than 10 min Vibrio sp. EJY3

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.006
-
 3,6-anhydro-alpha-L-galactopyranose at pH 8.0 and 20°C Vibrio sp. EJY3

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Vibrio sp. EJY3

pH Range

pH Minimum pH Maximum Comment Organism
6 12 the enzyme shows over 90% of its maximum within a small pH range from 8.0 to 11.0 and more than 60% activity between pH 6.0 and 12.0. At pH values lower than 5.0, activity of the enzyme is drastically reduced Vibrio sp. EJY3

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
 the enzyme shows 50% activity after 12 h at 20°C and pH 8.0 Vibrio sp. EJY3

Cofactor

Cofactor Comment Organism Structure
NADP+ dependent on Vibrio sp. EJY3

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.032
-
 3,6-anhydro-alpha-L-galactopyranose at pH 8.0 and 20°C Vibrio sp. EJY3