BRENDA - Enzyme Database show
show all sequences of 1.2.1.99

A novel NAD+-dependent aldehyde dehydrogenase encoded by the puuC gene of Klebsiella pneumoniae DSM 2026 that utilizes 3-hydroxypropionaldehyde as a substrate

Raj, S.; Rathnasingh, C.; Jung, W.; Selvakumar, E.; Park, S.; Biotechnol. Bioprocess Eng. 15, 131-138 (2010)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
1 mM, activity increases by 61%
Klebsiella pneumoniae
dithiothreitol
1 mM, activity increases by 47.5%
Klebsiella pneumoniae
Semicarbazide hydrochloride
1 mM, activity increases by 19%
Klebsiella pneumoniae
Application
Application
Commentary
Organism
synthesis
PuuC is an active enzyme that can be utilized effectively for several purposes, including biological production of 3-hydroxypropionate
Klebsiella pneumoniae
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli
Klebsiella pneumoniae
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ag+
1 mM, complete inhibition
Klebsiella pneumoniae
Co2+
1 mM, 11% inhibition
Klebsiella pneumoniae
Cu2+
1 mM, complete inhibition
Klebsiella pneumoniae
EDTA
1 mM, 8.3% inhibition
Klebsiella pneumoniae
Fe2+
1 mM, 73.6% inhibition
Klebsiella pneumoniae
Hg2+
1 mM, complete inhibition
Klebsiella pneumoniae
Li+
1 mM, 9.4% inhibition
Klebsiella pneumoniae
Mg2+
1 mM, 6.3% inhibition
Klebsiella pneumoniae
NaCl
mM, 11% inhibition
Klebsiella pneumoniae
NH4Cl
8% inhibition
Klebsiella pneumoniae
Ni2+
1 mM, 10.5% inhibition
Klebsiella pneumoniae
Zn2+
1 mM, 64% inhibition
Klebsiella pneumoniae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.14
-
Valeraldehyde
pH 8.0, 45°C
Klebsiella pneumoniae
0.21
-
Isovaleraldehyde
pH 8.0, 45°C
Klebsiella pneumoniae
0.26
-
Butyraldehyde
pH 8.0, 45°C
Klebsiella pneumoniae
0.48
-
3-hydroxypropionaldehyde
pH 8.0, 45°C
Klebsiella pneumoniae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53800
-
x * 53800, SDS-PAGE
Klebsiella pneumoniae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-hydroxypropionaldehyde + NAD+ + H2O
Klebsiella pneumoniae
the enzyme might play a role in putresine and propionate metabolism
3-hydroxypropanoate + NADH + 2 H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Klebsiella pneumoniae
-
-
-
Klebsiella pneumoniae DSM 2026
-
-
-
Purification (Commentary)
Commentary
Organism
-
Klebsiella pneumoniae
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
22.25
-
Vmax: 3-hydroxypropionaldehyde, pH 8.0, 45°C
Klebsiella pneumoniae
28.61
-
Vmax: isovaleraldehyde, pH 8.0, 45°C
Klebsiella pneumoniae
33.26
-
Vmax: valeraldehyde, pH 8.0, 45°C
Klebsiella pneumoniae
34.08
-
Vmax: butyraldehyde, pH 8.0, 45°C
Klebsiella pneumoniae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-furaldehyde + NAD+ + H2O
22% of the activity with 3-hydroxypropionaldehyde
711536
Klebsiella pneumoniae
furan-2-carboxylate + NADH + 2 H+
-
-
-
?
3-hydroxypropionaldehyde + NAD+ + H2O
-
711536
Klebsiella pneumoniae
3-hydroxypropanoate + NADH + 2 H+
-
-
-
?
3-hydroxypropionaldehyde + NAD+ + H2O
the enzyme might play a role in putresine and propionate metabolism
711536
Klebsiella pneumoniae
3-hydroxypropanoate + NADH + 2 H+
-
-
-
?
acetaldehyde + NAD+ + H2O
-
711536
Klebsiella pneumoniae
acetate + NADH + H+
-
-
-
?
benzaldehyde + NAD+ + H2O
35% of the activity with 3-hydroxypropionaldehyde
711536
Klebsiella pneumoniae
benzoate + NADH + H+
-
-
-
?
butyraldehyde + NAD+ + H2O
-
711536
Klebsiella pneumoniae
butyrate + NADH + 2 H+
-
-
-
?
isovaleraldehyde + NAD+ + H2O
-
711536
Klebsiella pneumoniae
3-methylbutanoate + NADH + 2 H+
-
-
-
?
additional information
no activity with formaldehyde
711536
Klebsiella pneumoniae
?
-
-
-
-
propionaldehyde + NAD+ + H2O
-
711536
Klebsiella pneumoniae
propionate + NADH + 2 H+
-
-
-
?
valeraldehyde + NAD+ + H2O
most preferred substrate
711536
Klebsiella pneumoniae
pentanoate + NADH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 53800, SDS-PAGE
Klebsiella pneumoniae
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
-
Klebsiella pneumoniae
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
60
20°C: about 30% of maximal activity, 60°C: about 35% of maximal activity
Klebsiella pneumoniae
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
becomes unstable at 60°C
Klebsiella pneumoniae
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
19.81
-
3-hydroxypropionaldehyde
pH 8.0, 45°C
Klebsiella pneumoniae
25.47
-
Isovaleraldehyde
pH 8.0, 45°C
Klebsiella pneumoniae
29.61
-
Valeraldehyde
pH 8.0, 45°C
Klebsiella pneumoniae
30.34
-
Butyraldehyde
pH 8.0, 45°C
Klebsiella pneumoniae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Klebsiella pneumoniae
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
9
pH 7.0: about 50% of maximal activity, pH 9.0: about 80% of maximal activity
Klebsiella pneumoniae
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
preferred coenzyme over NADP+
Klebsiella pneumoniae
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
1 mM, activity increases by 61%
Klebsiella pneumoniae
dithiothreitol
1 mM, activity increases by 47.5%
Klebsiella pneumoniae
Semicarbazide hydrochloride
1 mM, activity increases by 19%
Klebsiella pneumoniae
Application (protein specific)
Application
Commentary
Organism
synthesis
PuuC is an active enzyme that can be utilized effectively for several purposes, including biological production of 3-hydroxypropionate
Klebsiella pneumoniae
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Klebsiella pneumoniae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
preferred coenzyme over NADP+
Klebsiella pneumoniae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ag+
1 mM, complete inhibition
Klebsiella pneumoniae
Co2+
1 mM, 11% inhibition
Klebsiella pneumoniae
Cu2+
1 mM, complete inhibition
Klebsiella pneumoniae
EDTA
1 mM, 8.3% inhibition
Klebsiella pneumoniae
Fe2+
1 mM, 73.6% inhibition
Klebsiella pneumoniae
Hg2+
1 mM, complete inhibition
Klebsiella pneumoniae
Li+
1 mM, 9.4% inhibition
Klebsiella pneumoniae
Mg2+
1 mM, 6.3% inhibition
Klebsiella pneumoniae
NaCl
mM, 11% inhibition
Klebsiella pneumoniae
NH4Cl
8% inhibition
Klebsiella pneumoniae
Ni2+
1 mM, 10.5% inhibition
Klebsiella pneumoniae
Zn2+
1 mM, 64% inhibition
Klebsiella pneumoniae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.14
-
Valeraldehyde
pH 8.0, 45°C
Klebsiella pneumoniae
0.21
-
Isovaleraldehyde
pH 8.0, 45°C
Klebsiella pneumoniae
0.26
-
Butyraldehyde
pH 8.0, 45°C
Klebsiella pneumoniae
0.48
-
3-hydroxypropionaldehyde
pH 8.0, 45°C
Klebsiella pneumoniae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53800
-
x * 53800, SDS-PAGE
Klebsiella pneumoniae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-hydroxypropionaldehyde + NAD+ + H2O
Klebsiella pneumoniae
the enzyme might play a role in putresine and propionate metabolism
3-hydroxypropanoate + NADH + 2 H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Klebsiella pneumoniae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
22.25
-
Vmax: 3-hydroxypropionaldehyde, pH 8.0, 45°C
Klebsiella pneumoniae
28.61
-
Vmax: isovaleraldehyde, pH 8.0, 45°C
Klebsiella pneumoniae
33.26
-
Vmax: valeraldehyde, pH 8.0, 45°C
Klebsiella pneumoniae
34.08
-
Vmax: butyraldehyde, pH 8.0, 45°C
Klebsiella pneumoniae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-furaldehyde + NAD+ + H2O
22% of the activity with 3-hydroxypropionaldehyde
711536
Klebsiella pneumoniae
furan-2-carboxylate + NADH + 2 H+
-
-
-
?
3-hydroxypropionaldehyde + NAD+ + H2O
-
711536
Klebsiella pneumoniae
3-hydroxypropanoate + NADH + 2 H+
-
-
-
?
3-hydroxypropionaldehyde + NAD+ + H2O
the enzyme might play a role in putresine and propionate metabolism
711536
Klebsiella pneumoniae
3-hydroxypropanoate + NADH + 2 H+
-
-
-
?
acetaldehyde + NAD+ + H2O
-
711536
Klebsiella pneumoniae
acetate + NADH + H+
-
-
-
?
benzaldehyde + NAD+ + H2O
35% of the activity with 3-hydroxypropionaldehyde
711536
Klebsiella pneumoniae
benzoate + NADH + H+
-
-
-
?
butyraldehyde + NAD+ + H2O
-
711536
Klebsiella pneumoniae
butyrate + NADH + 2 H+
-
-
-
?
isovaleraldehyde + NAD+ + H2O
-
711536
Klebsiella pneumoniae
3-methylbutanoate + NADH + 2 H+
-
-
-
?
additional information
no activity with formaldehyde
711536
Klebsiella pneumoniae
?
-
-
-
-
propionaldehyde + NAD+ + H2O
-
711536
Klebsiella pneumoniae
propionate + NADH + 2 H+
-
-
-
?
valeraldehyde + NAD+ + H2O
most preferred substrate
711536
Klebsiella pneumoniae
pentanoate + NADH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 53800, SDS-PAGE
Klebsiella pneumoniae
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
-
Klebsiella pneumoniae
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
60
20°C: about 30% of maximal activity, 60°C: about 35% of maximal activity
Klebsiella pneumoniae
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
60
-
becomes unstable at 60°C
Klebsiella pneumoniae
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
19.81
-
3-hydroxypropionaldehyde
pH 8.0, 45°C
Klebsiella pneumoniae
25.47
-
Isovaleraldehyde
pH 8.0, 45°C
Klebsiella pneumoniae
29.61
-
Valeraldehyde
pH 8.0, 45°C
Klebsiella pneumoniae
30.34
-
Butyraldehyde
pH 8.0, 45°C
Klebsiella pneumoniae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Klebsiella pneumoniae
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
9
pH 7.0: about 50% of maximal activity, pH 9.0: about 80% of maximal activity
Klebsiella pneumoniae
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
41.44
-
3-hydroxypropionaldehyde
pH 8.0, 45°C
Klebsiella pneumoniae
117.2
-
Butyraldehyde
pH 8.0, 45°C
Klebsiella pneumoniae
121.9
-
Isovaleraldehyde
pH 8.0, 45°C
Klebsiella pneumoniae
207.1
-
Valeraldehyde
pH 8.0, 45°C
Klebsiella pneumoniae
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
41.44
-
3-hydroxypropionaldehyde
pH 8.0, 45°C
Klebsiella pneumoniae
117.2
-
Butyraldehyde
pH 8.0, 45°C
Klebsiella pneumoniae
121.9
-
Isovaleraldehyde
pH 8.0, 45°C
Klebsiella pneumoniae
207.1
-
Valeraldehyde
pH 8.0, 45°C
Klebsiella pneumoniae
Other publictions for EC 1.2.1.99
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724621
Ashok
Production of 3-hydroxypropion ...
Klebsiella pneumoniae
Biotechnol. Bioeng.
110
511-524
2013
-
1
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
740617
Schneider
Pathway and enzyme redundancy ...
Escherichia coli
J. Bacteriol.
194
4080-4088
2012
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
710985
Ashok
Development of recombinant Kle ...
Klebsiella pneumoniae, Klebsiella pneumoniae DSM 2026
Appl. Microbiol. Biotechnol.
90
1253-1265
2011
-
1
1
-
-
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
711536
Raj
-
A novel NAD+-dependent aldehyd ...
Klebsiella pneumoniae, Klebsiella pneumoniae DSM 2026
Biotechnol. Bioprocess Eng.
15
131-138
2010
3
1
1
-
-
-
12
4
-
-
1
1
-
6
-
-
1
-
-
-
4
-
10
1
1
1
1
4
1
1
-
1
-
-
-
3
1
1
1
-
-
-
-
12
-
4
-
-
1
1
-
-
-
1
-
-
4
-
10
1
1
1
1
4
1
1
-
-
-
-
-
-
4
4
690591
Jo
Cloning, expression, and chara ...
Escherichia coli
Appl. Microbiol. Biotechnol.
81
51-60
2008
2
-
1
-
-
-
15
9
-
-
-
1
-
1
-
-
1
-
-
-
9
-
9
-
1
1
-
9
1
1
-
2
-
-
-
2
-
1
2
-
-
-
-
15
-
9
-
-
-
1
-
-
-
1
-
-
9
-
9
-
1
1
-
9
1
1
-
-
-
-
-
-
9
9
713388
Raj
-
Production of 3-hydroxypropion ...
Escherichia coli
Proc. Biochem.
32
1440-1446
2008
-
1
1
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659502
Kurihara
A novel putrescine utilization ...
Escherichia coli
J. Biol. Chem.
280
4602-4608
2005
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-