BRENDA - Enzyme Database show
show all sequences of 1.2.1.99

Production of 3-hydroxypropionic acid from glycerol by a novel recombinant Escherichia coli BL21 strain

Raj, S.M.; Rathnasingh, C.; Jo, J.-E.; Park, S.; Proc. Biochem. 32, 1440-1446 (2008)
No PubMed abstract available

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
3-hydroxypropionic acid is an important compound from which several commodity and specialty chemicals can be generated. The recombinant strain SH254 producing 3-hydroxypropionate from glycerol is developed by cloning of dhaB and aldH genes in Escherichia coli BL21 under different regulatory promoters. The recombinant SH254 can accumulate 3-hydroxypropionic acid from glycerol at 6.5 mmol/l in 30 h
Escherichia coli
Cloned(Commentary)
Commentary
Organism
the recombinant strain SH254 producing 3-hydroxypropionate from glycerol is developed by cloning of dhaB and aldH genes in Escherichia coli BL21 under different regulatory promoters
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-hydroxypropionaldehyde + NAD+ + H2O
Escherichia coli
-
3-hydroxypropanoate + NADH + 2 H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P23883
-
-
Purification (Commentary)
Commentary
Organism
partial
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-hydroxypropionaldehyde + NAD+ + H2O
-
713388
Escherichia coli
3-hydroxypropanoate + NADH + 2 H+
-
-
-
?
Application (protein specific)
Application
Commentary
Organism
synthesis
3-hydroxypropionic acid is an important compound from which several commodity and specialty chemicals can be generated. The recombinant strain SH254 producing 3-hydroxypropionate from glycerol is developed by cloning of dhaB and aldH genes in Escherichia coli BL21 under different regulatory promoters. The recombinant SH254 can accumulate 3-hydroxypropionic acid from glycerol at 6.5 mmol/l in 30 h
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
the recombinant strain SH254 producing 3-hydroxypropionate from glycerol is developed by cloning of dhaB and aldH genes in Escherichia coli BL21 under different regulatory promoters
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-hydroxypropionaldehyde + NAD+ + H2O
Escherichia coli
-
3-hydroxypropanoate + NADH + 2 H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-hydroxypropionaldehyde + NAD+ + H2O
-
713388
Escherichia coli
3-hydroxypropanoate + NADH + 2 H+
-
-
-
?
Other publictions for EC 1.2.1.99
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724621
Ashok
Production of 3-hydroxypropion ...
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Biotechnol. Bioeng.
110
511-524
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1
1
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-
1
-
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1
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3
-
2
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-
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1
1
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1
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-
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-
3
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Appl. Microbiol. Biotechnol.
90
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2
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6
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2
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1
1
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2
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2
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711536
Raj
-
A novel NAD+-dependent aldehyd ...
Klebsiella pneumoniae, Klebsiella pneumoniae DSM 2026
Biotechnol. Bioprocess Eng.
15
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2010
3
1
1
-
-
-
12
4
-
-
1
1
-
6
-
-
1
-
-
-
4
-
10
1
1
1
1
4
1
1
-
1
-
-
-
3
1
1
1
-
-
-
-
12
-
4
-
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1
1
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1
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4
-
10
1
1
1
1
4
1
1
-
-
-
-
-
-
4
4
690591
Jo
Cloning, expression, and chara ...
Escherichia coli
Appl. Microbiol. Biotechnol.
81
51-60
2008
2
-
1
-
-
-
15
9
-
-
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1
-
1
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1
-
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9
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9
-
1
1
-
9
1
1
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2
-
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2
-
1
2
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15
-
9
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1
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-
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1
-
-
9
-
9
-
1
1
-
9
1
1
-
-
-
-
-
-
9
9
713388
Raj
-
Production of 3-hydroxypropion ...
Escherichia coli
Proc. Biochem.
32
1440-1446
2008
-
1
1
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-
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1
-
1
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1
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1
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1
1
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1
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1
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1
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659502
Kurihara
A novel putrescine utilization ...
Escherichia coli
J. Biol. Chem.
280
4602-4608
2005
-
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-
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1
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1
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1
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