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show all sequences of 1.2.1.99

Production of 3-hydroxypropionic acid from glycerol by recombinant Klebsiella pneumoniae DELTAdhaTDELTAyqhD which can produce vitamin B12 naturally

Ashok, S.; Sankaranarayanan, M.; Ko, Y.; Jae, K.E.; Ainala, S.K.; Kumar, V.; Park, S.; Biotechnol. Bioeng. 110, 511-524 (2013)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
3-hydroxypropionic acid is an important platform chemical that can be used to synthesize a range of chemical compounds, such as acrylic acid, 1,3-propanediol, methyl acrylate, malonic acid, acrylamide, and hydroxyamides
Klebsiella pneumoniae
Cloned(Commentary)
Commentary
Organism
PuuC of Klebsiella pneumoniae alone or with coenzyme B12-dependent glycerol dehydratase, DhaB overexpressed homologously, and two major oxidoreductases, DhaT and YqhD, disrupted resulting in KpC_DELTA_dhaT_DELTA_yqhD strain and KpBC_DELTA_dhaT_DELTA_yqhD strain, overexpressing the glycerol dehydratase DhaB, too
Klebsiella pneumoniae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53000
-
PuuC protein in recombinant Klebsiella pneumoniae, SDS–PAGE
Klebsiella pneumoniae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Klebsiella pneumoniae
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
activity varies depending on the type of strain and culture conditions, e.g. aeration conditions and working volume
Klebsiella pneumoniae
1.5
-
recombinant KpBC_DELTA_dhaT_DELTA_yqhD strain, pH 8.0, 45°C
Klebsiella pneumoniae
2.5
-
recombinant KpC_DELTA_dhaT_DELTA_yqhD strain, pH 8.0, 45°C
Klebsiella pneumoniae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-hydroxypropionaldehyde + NAD+ + H2O
3-hydroxypropionaldehyde i.e. 3-HPA, 3-hydroxypropionic acid i.e. 3-HP, PuuC overexpression enhances the glycerol carbon flux towards 3-hydroxypropionaldehyde
724621
Klebsiella pneumoniae
3-hydroxypropanoate + NADH + 2 H+
-
-
-
?
additional information
development and evaluation recombinant Klebsiella pneumoniae, for the production of 3-hydroxypropionic acid from glycerol, therefor PuuC together with a coenzyme B12-dependent glycerol dehydratase are overexpressed while two major oxidoreductases, DhaT and YqhD, are disrupted eliminating the production of 1,3-propanediol out of 3-hydroxypropionaldehyde
724621
Klebsiella pneumoniae
?
-
-
-
?
Application (protein specific)
Application
Commentary
Organism
synthesis
3-hydroxypropionic acid is an important platform chemical that can be used to synthesize a range of chemical compounds, such as acrylic acid, 1,3-propanediol, methyl acrylate, malonic acid, acrylamide, and hydroxyamides
Klebsiella pneumoniae
Cloned(Commentary) (protein specific)
Commentary
Organism
PuuC of Klebsiella pneumoniae alone or with coenzyme B12-dependent glycerol dehydratase, DhaB overexpressed homologously, and two major oxidoreductases, DhaT and YqhD, disrupted resulting in KpC_DELTA_dhaT_DELTA_yqhD strain and KpBC_DELTA_dhaT_DELTA_yqhD strain, overexpressing the glycerol dehydratase DhaB, too
Klebsiella pneumoniae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53000
-
PuuC protein in recombinant Klebsiella pneumoniae, SDS–PAGE
Klebsiella pneumoniae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
activity varies depending on the type of strain and culture conditions, e.g. aeration conditions and working volume
Klebsiella pneumoniae
1.5
-
recombinant KpBC_DELTA_dhaT_DELTA_yqhD strain, pH 8.0, 45°C
Klebsiella pneumoniae
2.5
-
recombinant KpC_DELTA_dhaT_DELTA_yqhD strain, pH 8.0, 45°C
Klebsiella pneumoniae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-hydroxypropionaldehyde + NAD+ + H2O
3-hydroxypropionaldehyde i.e. 3-HPA, 3-hydroxypropionic acid i.e. 3-HP, PuuC overexpression enhances the glycerol carbon flux towards 3-hydroxypropionaldehyde
724621
Klebsiella pneumoniae
3-hydroxypropanoate + NADH + 2 H+
-
-
-
?
additional information
development and evaluation recombinant Klebsiella pneumoniae, for the production of 3-hydroxypropionic acid from glycerol, therefor PuuC together with a coenzyme B12-dependent glycerol dehydratase are overexpressed while two major oxidoreductases, DhaT and YqhD, are disrupted eliminating the production of 1,3-propanediol out of 3-hydroxypropionaldehyde
724621
Klebsiella pneumoniae
?
-
-
-
?
Other publictions for EC 1.2.1.99
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724621
Ashok
Production of 3-hydroxypropion ...
Klebsiella pneumoniae
Biotechnol. Bioeng.
110
511-524
2013
-
1
1
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1
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1
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3
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2
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1
1
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1
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3
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2
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740617
Schneider
Pathway and enzyme redundancy ...
Escherichia coli
J. Bacteriol.
194
4080-4088
2012
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1
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2
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1
1
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710985
Ashok
Development of recombinant Kle ...
Klebsiella pneumoniae, Klebsiella pneumoniae DSM 2026
Appl. Microbiol. Biotechnol.
90
1253-1265
2011
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1
1
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2
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6
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2
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2
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711536
Raj
-
A novel NAD+-dependent aldehyd ...
Klebsiella pneumoniae, Klebsiella pneumoniae DSM 2026
Biotechnol. Bioprocess Eng.
15
131-138
2010
3
1
1
-
-
-
12
4
-
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1
1
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6
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1
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4
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10
1
1
1
1
4
1
1
-
1
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3
1
1
1
-
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12
-
4
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1
1
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1
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4
-
10
1
1
1
1
4
1
1
-
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4
4
690591
Jo
Cloning, expression, and chara ...
Escherichia coli
Appl. Microbiol. Biotechnol.
81
51-60
2008
2
-
1
-
-
-
15
9
-
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1
-
1
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1
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9
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9
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1
1
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9
1
1
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2
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2
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1
2
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15
-
9
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1
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1
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9
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9
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1
1
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9
1
1
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9
9
713388
Raj
-
Production of 3-hydroxypropion ...
Escherichia coli
Proc. Biochem.
32
1440-1446
2008
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1
1
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1
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1
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659502
Kurihara
A novel putrescine utilization ...
Escherichia coli
J. Biol. Chem.
280
4602-4608
2005
-
-
-
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1
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1
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