BRENDA - Enzyme Database
show all sequences of 1.2.2.B2

Purification and properties of the methanol dehydrogenase from Methylophilus methylotrophus

Ghosh, R.; Quayle, J.R.; Biochem. J. 199, 245-250 (1981) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.01
-
formaldehyde
-
Methylophilus methylotrophus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
the enzyme is loosely bound to the intracellular membrane
Methylophilus methylotrophus
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
62000
-
2 * 62000, SDS-PAGE
Methylophilus methylotrophus
115000
-
gel filtration
Methylophilus methylotrophus
Organism
Organism
UniProt
Commentary
Textmining
Methylophilus methylotrophus
P38539 and P38540
P38539 (large subunit, alpha) and P38540 (small subunit, beta)
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Methylophilus methylotrophus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
formaldehyde + oxidized 2,6-dichlorophenolindophenol
-
389944
Methylophilus methylotrophus
formate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
additional information
acetaldehyde is oxidized very slowly
389944
Methylophilus methylotrophus
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 62000, SDS-PAGE
Methylophilus methylotrophus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.01
-
formaldehyde
-
Methylophilus methylotrophus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
the enzyme is loosely bound to the intracellular membrane
Methylophilus methylotrophus
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
62000
-
2 * 62000, SDS-PAGE
Methylophilus methylotrophus
115000
-
gel filtration
Methylophilus methylotrophus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Methylophilus methylotrophus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
formaldehyde + oxidized 2,6-dichlorophenolindophenol
-
389944
Methylophilus methylotrophus
formate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
additional information
acetaldehyde is oxidized very slowly
389944
Methylophilus methylotrophus
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 62000, SDS-PAGE
Methylophilus methylotrophus
Other publictions for EC 1.2.2.B2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
687432
Grosse
Purification and properties of ...
Methylosinus sp., Methylosinus sp. WI 14
J. Basic Microbiol.
38
189-196
1998
2
-
-
-
-
-
4
-
1
-
2
-
-
2
-
-
1
-
-
-
1
2
8
1
-
1
1
1
-
1
1
-
1
-
-
-
2
-
-
1
-
-
-
-
4
-
-
1
-
2
-
-
-
-
1
-
-
1
2
8
1
1
1
1
-
1
1
-
-
-
-
-
-
-
-
692909
Grosse
Purification and properties of ...
Methylocystis sp., Methylocystis sp. WI14
J. Basic Microbiol.
8
189-196
1998
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
1
-
-
-
1
2
8
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
1
2
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389944
Ghosh
Purification and properties of ...
Methylophilus methylotrophus
Biochem. J.
199
245-250
1981
-
-
-
-
-
-
-
1
1
-
2
-
-
1
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
2
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
389950
Ohta
Methanol dehydrogenase of Meth ...
Methylomonas sp. J
J. Biochem.
90
205-213
1981
-
-
-
-
-
-
-
1
-
-
2
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
1
-
-
2
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-