Activating Compound | Comment | Organism | Structure |
---|---|---|---|
methylamine | can replace NH4+ | Methylosinus sp. | |
NH4+ | absolute requirement, NH4+ can be replaced by methylamine but not by di- or triamines | Methylosinus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 38% inhibition at 0.1 mM | Methylosinus sp. | |
Fe2+ | complete inhibition at 1 mM | Methylosinus sp. | |
additional information | no or poor inhibition by Ca2+, Co2+, Li+, Mg2+, Mn2+, and KCN, EDTA, and DTE | Methylosinus sp. | |
phenazine methosulfate | 39% inhibition at 3 mM | Methylosinus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Methylosinus sp. | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
2 * 70000, SDS-PAGE | Methylosinus sp. |
140000 | - |
gel filtration | Methylosinus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylosinus sp. | - |
- |
- |
Methylosinus sp. WI 14 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 9fold to homogeneity by anion exchange chromatography, ammonium sulfate fractionation, and hydrophobic interaction chromatography, followed by ultrafiltration and gel filtration | Methylosinus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.12 | - |
purified enzyme | Methylosinus sp. |
Storage Stability | Organism |
---|---|
-18°C, purified native enzyme, 0.05 M sodium phosphate buffer, pH 7.5, loss of 10% of activity after 1 month | Methylosinus sp. |
4°C, purified native enzyme, 0.05 M sodium phosphate buffer, pH 7.5, loss of 30% of activity after 24 h | Methylosinus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + 2,6-dichlorophenolindophenol | with phenazine methosulfate | Methylosinus sp. | acetate + reduced 2,6-dichlorophenolindophenol | - |
? | |
acetaldehyde + 2,6-dichlorophenolindophenol | with phenazine methosulfate | Methylosinus sp. WI 14 | acetate + reduced 2,6-dichlorophenolindophenol | - |
? | |
formaldehyde + 2,6-dichlorophenolindophenol | with phenazine methosulfate | Methylosinus sp. | formate + reduced 2,6-dichlorophenolindophenol | - |
? | |
formaldehyde + 2,6-dichlorophenolindophenol | with phenazine methosulfate | Methylosinus sp. WI 14 | formate + reduced 2,6-dichlorophenolindophenol | - |
? | |
additional information | broad substrate specificity, MDH from Methylosinus sp. strain WI 14 oxidises only primary alcohols up to octanol and several aldehydes | Methylosinus sp. | ? | - |
? | |
additional information | broad substrate specificity, MDH from Methylosinus sp. strain WI 14 oxidises only primary alcohols up to octanol and several aldehydes | Methylosinus sp. WI 14 | ? | - |
? | |
propionaldehyde + 2,6-dichlorophenolindophenol | with phenazine methosulfate | Methylosinus sp. | propionate + reduced 2,6-dichlorophenolindophenol | - |
? | |
propionaldehyde + 2,6-dichlorophenolindophenol | with phenazine methosulfate | Methylosinus sp. WI 14 | propionate + reduced 2,6-dichlorophenolindophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 70000, SDS-PAGE | Methylosinus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
57 | - |
- |
Methylosinus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 60 | - |
Methylosinus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
30 min, purified enzyme, loss of 25% activity | Methylosinus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Methylosinus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | - |
Methylosinus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | prosthetic group, 1.3 molecules per enzyme molecule | Methylosinus sp. |