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Literature summary for 1.2.3.1 extracted from

  • Yamada, M.; Adachi, K.; Ogawa, N.; Kishino, S.; Ogawa, J.; Kataoka, M.; Shimizu, S.; Isobe, K.
    A new aldehyde oxidase catalyzing the conversion of glycolaldehyde to glycolate from Burkholderia sp. AIU 129 (2015), J. Biosci. Bioeng., 119, 410-415.
    View publication on PubMed
Search for more literature for 1.2.3.1

Inhibitors

Inhibitors Comment Organism Structure
8-hydroxyquinoline 1 mM, 20% residual activity Burkholderia sp. AIU 129
alpha,alpha'-dipyridyl 1 mM, 49% residual activity Burkholderia sp. AIU 129
Cu2+ 1 mM, 4% residual activity Burkholderia sp. AIU 129
Fe2+ 1 mM, no residual activity Burkholderia sp. AIU 129
Mo5+ 1 mM, 6% residual activity Burkholderia sp. AIU 129
phenylhydrazine 1 mM, 6% residual activity Burkholderia sp. AIU 129
Zn2+ 1 mM, 67% residual activity Burkholderia sp. AIU 129

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 benzaldehyde pH 7.0, 30°C Burkholderia sp. AIU 129
0.1
-
 butylaldehyde pH 7.0, 30°C Burkholderia sp. AIU 129
0.34
-
 acetaldehyde pH 7.0, 30°C Burkholderia sp. AIU 129
0.74
-
 propionaldehyde pH 7.0, 30°C Burkholderia sp. AIU 129
1
-
 glycolaldehyde pH 7.0, 30°C Burkholderia sp. AIU 129
12.3
-
 Glyoxal pH 7.0, 30°C Burkholderia sp. AIU 129
35.9
-
 formaldehyde pH 7.0, 30°C Burkholderia sp. AIU 129

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
-
 Burkholderia sp. AIU 129
36000
-
-
 Burkholderia sp. AIU 129
76000
-
-
 Burkholderia sp. AIU 129
130000
-
 gel filtration Burkholderia sp. AIU 129

Organism

Organism UniProt Comment Textmining
Burkholderia sp. AIU 129
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + O2 + H2O
-
 Burkholderia sp. AIU 129 acetate + H2O2
-
 ?
benzaldehyde + H2O + O2
-
 Burkholderia sp. AIU 129 benzoate + H2O2
-
 ?
butylaldehyde + H2O + O2
-
 Burkholderia sp. AIU 129 butanoate + H2O2
-
 ?
formaldehyde + H2O + O2
-
 Burkholderia sp. AIU 129 formate + H2O2
-
 ?
glycolaldehyde + H2O + O2
-
 Burkholderia sp. AIU 129 hydroxyacetic acid + H2O2
-
 ?
glyoxal + H2O + O2
-
 Burkholderia sp. AIU 129 glyoxylate + H2O2
-
 ?
additional information no substrates: glycolate and alcohols such as ethanol, 1-propanol, 2-methoxyethanol, ethylene glycol and glycerol Burkholderia sp. AIU 129 ?
-
 ?
propionaldehyde + H2O + O2
-
 Burkholderia sp. AIU 129 propionate + H2O2
-
 ?

Subunits

Subunits Comment Organism
heterotrimer 1 * 76000, alpha-subunit, plus 1 * 36000, beta-subunit, plus 1 * 14000, gamma-subunit Burkholderia sp. AIU 129

Synonyms

Synonyms Comment Organism
ALOD
-
 Burkholderia sp. AIU 129

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Burkholderia sp. AIU 129

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 30 min, morethan 50% residual activity Burkholderia sp. AIU 129

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Burkholderia sp. AIU 129

pH Range

pH Minimum pH Maximum Comment Organism
7 8 more than 70% of maximum activity Burkholderia sp. AIU 129