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Literature summary for 1.2.3.14 extracted from
- Identification of superoxide production by Arabidopsis thaliana aldehyde oxidases AAO1 and AAO3 (2012), Plant Mol. Biol., 80, 659-671.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cDNAs of AAO1 and AAO3 expressed in Pichia pastoris to obtain recombinant homodimeric AAO1 and AAO3 proteins with His6-tag | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cyanide | the ability of AAO1 and AAO3 to reduce 2,6-dichloroindophenol is abrogated when the enzymes are pre-treated with cyanide, NADH oxidation activity of AAO1 and AAO3 is highly sensitive to cyanide treatment | Arabidopsis thaliana |  |
| diphenylene iodonium | DPI i.e. diphenylene iodonium, in the presence of DPI aldehyde oxidation activities of AAO1 and AAO3 are strongly reduced to 116%, NADH oxidation activity of AAO1 and AAO3 is highly sensitive to DPI treatment | Arabidopsis thaliana | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q7G9P4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by affinity chromatography with nickel-nitrilotriacetic acid-agarose under native conditions, further purification by anion exchange chromatography | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| rosette leaf | extracts from drought stressed leaves, AAO3 | Arabidopsis thaliana | - |
| seedling | AAO1 | Arabidopsis thaliana | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
in the presence of diphenylene iodonium, aldehyde oxidation activities of AAO1 and AAO3 are strongly reduced to 116% | Arabidopsis thaliana |
| 0.093 | - |
heptaldehyde oxidation by AAO1, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
| 0.11 | - |
benzaldehyde oxidation by AAO1, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
| 0.146 | - |
benzaldehyde oxidation by AAO3, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
| 0.204 | - |
indole-3-carbaldehyde oxidation by AAO3, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
| 0.215 | - |
NADH oxidation by AAO1, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
| 0.515 | - |
abscisic aldehyde oxidation by AAO1, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
| 0.517 | - |
heptaldehyde oxidation by AAO3, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
| 0.53 | - |
NADH oxidation by AAO3, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
| 0.558 | - |
indole-3-carbaldehyde oxidation by AAO1, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
| 0.635 | - |
abscisic aldehyde oxidation by AAO3, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| abscisic aldehyde + 2,6-dichloroindophenol | 2,6-dichloroindophenol i.e. DCIP used as electron acceptor, natural electron acceptor is oxygen | Arabidopsis thaliana | abscisic acid + H2O2 | rate of H2O2 formation increases in the presence of superoxide dismutase, indicating that in addition to the two-electron reduction of molecular oxygen, AAO1 and AAO3 also catalyze a one-electron transfer to molecular oxygen, leading to the formation of superoxide ion, O2- | ? | |
| benzaldehyde + 2,6-dichloroindophenol | 2,6-dichloroindophenol i.e. DCIP used as electron acceptor, natural electron acceptor is oxygen | Arabidopsis thaliana | benzoic acid + H2O2 | rate of H2O2 formation increases in the presence of superoxide dismutase, indicating that in addition to the two-electron reduction of molecular oxygen, AAO1 and AAO3 also catalyze a one-electron transfer to molecular oxygen, leading to the formation of superoxide ion, O2- | ? | |
| heptaldehyde + 2,6-dichloroindophenol | 2,6-dichloroindophenol i.e. DCIP used as electron acceptor, natural electron acceptor is oxygen | Arabidopsis thaliana | heptanoic acid + H2O2 | rate of H2O2 formation increases in the presence of superoxide dismutase, indicating that in addition to the two-electron reduction of molecular oxygen, AAO1 and AAO3 also catalyze a one-electron transfer to molecular oxygen, leading to the formation of superoxide ion, O2- | ? | |
| indole-3-carbaldehyde + 2,6-dichloroindophenol | 2,6-dichloroindophenol i.e. DCIP used as electron acceptor, natural electron acceptor is oxygen | Arabidopsis thaliana | indole-3-carboxylate + H2O2 | rate of H2O2 formation increases in the presence of superoxide dismutase, indicating that in addition to the two-electron reduction of molecular oxygen, AAO1 and AAO3 also catalyze a one-electron transfer to molecular oxygen, leading to the formation of superoxide ion, O2- | ? | |
| additional information | confirmation of superoxide generation by AAO1 and AAO3 by monitoring the reduction of the tetrazolium salt XTT due to O2- | Arabidopsis thaliana | ? | - |
? | |
| NADH + O2 | oxidation of NADH by AAO1 and AAO3, no oxidation of NADPH by AAO1 or AAO3 | Arabidopsis thaliana | NAD+ + O2- | for confirmation, O2--dependent reduction of cytochrome c monitored, oxidation of NADH by AAO1 and AAO3 does not result in detectable levels of H2O2 | ? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AAO1 | - |
Arabidopsis thaliana |
| AAO3 | - |
Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | presence of all prosthetic groups confirmed by UVvis spectroscopy | Arabidopsis thaliana |
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