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Literature summary for 1.2.3.15 extracted from

  • Kadowaki, M.A.S.; Ortiz de Godoy, M.; Kumagai, P.S.; da Costa-Filho, A.J.; Mort, A.; Prade, R.A.; Polikarpov, I.
    Characterization of a new glyoxal oxidase from the thermophilic fungus Myceliophthora thermophila M77 hydrogen peroxide production retained in 5-hydroxymethylfurfural oxidation (2018), Catalysts, 8, 476 .
No PubMed abstract available
Search for more literature for 1.2.3.15

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of structure. An N-terminal beta-propeller structure containing the catalytic copper center is linked to a C-terminal immunoglobulin-like domain, with both domains being involved in forming the active site. Residues His804, His889, and Tyr803 compose the first-shell coordination of the copper ion and Cys522-Tyr581 stabilize the free-radical species Thermothelomyces thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
12.8
-
 methylglyoxal pH 6, 50°C Thermothelomyces thermophilus
20.2
-
 5-hydroxymethylfurfural pH 6, 50°C Thermothelomyces thermophilus
471.3
-
 glycerol pH 6, 50°C Thermothelomyces thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
copper protein contains a radical-coupled copper complex, a mononuclear Cu(II) center with an axial coordination Thermothelomyces thermophilus

Organism

Organism UniProt Comment Textmining
Thermothelomyces thermophilus G2Q335
-
-
Thermothelomyces thermophilus DSM 1799 G2Q335
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-hydroxymethylfurfural + H2O + O2
-
 Thermothelomyces thermophilus 2,5-diformylfuran + H2O2
-
 ?
5-hydroxymethylfurfural + H2O + O2
-
 Thermothelomyces thermophilus DSM 1799 2,5-diformylfuran + H2O2
-
 ?
formaldehyde + H2O + O2 4% of the activity with 5-hydroxymethylfurfural Thermothelomyces thermophilus formate + H2O2
-
 ?
formaldehyde + H2O + O2 4% of the activity with 5-hydroxymethylfurfural Thermothelomyces thermophilus DSM 1799 formate + H2O2
-
 ?
glycerol + H2O + O2 16% of the activity with 5-hydroxymethylfurfural Thermothelomyces thermophilus ? + H2O2
-
 ?
glycerol + H2O + O2 16% of the activity with 5-hydroxymethylfurfural Thermothelomyces thermophilus DSM 1799 ? + H2O2
-
 ?
methylglyoxal + H2O + O2 96% of the activity with 5-hydroxymethylfurfural Thermothelomyces thermophilus methylglyoxylate + H2O2
-
 ?
methylglyoxal + H2O + O2 96% of the activity with 5-hydroxymethylfurfural Thermothelomyces thermophilus DSM 1799 methylglyoxylate + H2O2
-
 ?
additional information no substrates: furfural, 2,5-diformylfuran, glutaraldehyde Thermothelomyces thermophilus ?
-
-
additional information no substrates: furfural, 2,5-diformylfuran, glutaraldehyde Thermothelomyces thermophilus DSM 1799 ?
-
-

Synonyms

Synonyms Comment Organism
GLOX
-
 Thermothelomyces thermophilus
MYCTH_2294895
-
 Thermothelomyces thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Thermothelomyces thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 15 min, 50% loss of activity Thermothelomyces thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12.6
-
 methylglyoxal pH 6, 50°C Thermothelomyces thermophilus
15.9
-
 5-hydroxymethylfurfural pH 6, 50°C Thermothelomyces thermophilus
34.1
-
 glycerol pH 6, 50°C Thermothelomyces thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Thermothelomyces thermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.07
-
 glycerol pH 6, 50°C Thermothelomyces thermophilus
0.86
-
 5-hydroxymethylfurfural pH 6, 50°C Thermothelomyces thermophilus
0.99
-
 methylglyoxal pH 6, 50°C Thermothelomyces thermophilus