Application | Comment | Organism |
---|---|---|
additional information | pyruvate oxidase is not only a stationary-phase enzyme. Removal of the poxB gene affects the central metabolism at the enzyme level | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | poxB mutant, absence of pyruvate oxidase activity, which reduces glucose uptake rate, biomass yield, acetate production and cell growth rate, and increases O2 consumption and CO2 evolution. Glucokinase activity increases, whereas the flux through glycolysis is reduced due to down-regulation of other glycolytic enzymes such as 6-phosphofructosekinase and fructose bisphosphate aldolase. TCA cycle enzymes such as citrate synthase and malate dehydrogenase are repressed in the poxB mutant when cells are cultivated in LB medium. Activation of glucose-6-phosphate dehydrogenase and acetyl-CoA synthetase | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
strains BW25113 and JW0855 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + H2O | - |
Escherichia coli | acetate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
poxB | - |
Escherichia coli |