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Literature summary for 1.2.3.4 extracted from

  • Koyama, H.
    Purification and characterization of oxalate oxidase from Pseudomonas sp. OX-53 (1988), Agric. Biol. Chem., 52, 743-748.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
FAD 0.1 mM concentration 66% activation Pseudomonas sp.
FMN 0.1 mM concentration 88% activation Pseudomonas sp.
hydroquinone 0.1 mM concentration 123% activation Pseudomonas sp.
MnSO4 0.1 mM concentration 75% activation Pseudomonas sp.
o-phenylenediamine 0.1 mM concentration 505% activation Pseudomonas sp.
phenylhydrazine 0.1 mM concentration 280% activation Pseudomonas sp.
riboflavin 0.1 mM concentration 111% activation Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
8-hydroxyquinoline 0.1 mM concentration 41% inhibition Pseudomonas sp.
alpha,alpha'-dipyridyl 0.1 mM concentration 31% inhibition Pseudomonas sp.
azide 0.1 mM concentration 10% inhibition Pseudomonas sp.
CuSO4 0.1 mM concentration 36% inhibition Pseudomonas sp.
EDTA 0.1 mM concentration 42% inhibition Pseudomonas sp.
HgCl2 0.1 mM 36% inhibition Pseudomonas sp.
hydroxylamine 0.1 mM concentration 100% inhibition Pseudomonas sp.
iodacetamide 0.1 mM concentration 62% inhibition Pseudomonas sp.
KBr 1 mM concentration 58% inhibition Pseudomonas sp.
KI 1 mM concentration 94% inhibition Pseudomonas sp.
o-phenanthroline 0.1 mM concentration 26% inhibition Pseudomonas sp.
Semicarbazide 0.1 mM concentration 57% inhibition Pseudomonas sp.
ZnSO4 0.1 mM concentration 12% inhibition Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
9.5
-
oxalic acid
-
Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 0.09 atoms per subunit Pseudomonas sp.
Mn2+ 1.12 atoms per subunit Pseudomonas sp.
Zn2+ 0.36 atoms per subunit Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
alpha8, 8 * 38000, SDS-PAGE Pseudomonas sp.
320000
-
gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. OX-53
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
630
-
O2 consumption Pseudomonas sp.

Storage Stability

Storage Stability Organism
-20°C, potassium phosphate buffer pH 6.5, 6 months, no activity loss Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-malic acid + O2
-
Pseudomonas sp. ?
-
?
DL-malic acid + O2
-
Pseudomonas sp. OX-53 ?
-
?
glyoxylic acid + O2 + 2 H+
-
Pseudomonas sp. CO2 + formaldehyde + H2O2
-
?
glyoxylic acid + O2 + 2 H+
-
Pseudomonas sp. OX-53 CO2 + formaldehyde + H2O2
-
?
maleic acid + O2
-
Pseudomonas sp. ?
-
?
maleic acid + O2
-
Pseudomonas sp. OX-53 ?
-
?
oxalic acid + O2 + 2 H+
-
Pseudomonas sp. 2 CO2 + H2O2
-
ir
oxalic acid + O2 + 2 H+
-
Pseudomonas sp. OX-53 2 CO2 + H2O2
-
ir

Subunits

Subunits Comment Organism
octamer alpha8, 8 * 38000, SDS-PAGE Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
after 10 min 100% activity Pseudomonas sp.
60
-
after 10 min, 76% activity Pseudomonas sp.
70
-
after 10 min 9% activity Pseudomonas sp.
89
-
after 3 min 49% activity Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
-
Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 7
-
Pseudomonas sp.