Activating Compound | Comment | Organism | Structure |
---|---|---|---|
FAD | 0.1 mM concentration 66% activation | Pseudomonas sp. | |
FMN | 0.1 mM concentration 88% activation | Pseudomonas sp. | |
hydroquinone | 0.1 mM concentration 123% activation | Pseudomonas sp. | |
MnSO4 | 0.1 mM concentration 75% activation | Pseudomonas sp. | |
o-phenylenediamine | 0.1 mM concentration 505% activation | Pseudomonas sp. | |
phenylhydrazine | 0.1 mM concentration 280% activation | Pseudomonas sp. | |
riboflavin | 0.1 mM concentration 111% activation | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-hydroxyquinoline | 0.1 mM concentration 41% inhibition | Pseudomonas sp. | |
alpha,alpha'-dipyridyl | 0.1 mM concentration 31% inhibition | Pseudomonas sp. | |
azide | 0.1 mM concentration 10% inhibition | Pseudomonas sp. | |
CuSO4 | 0.1 mM concentration 36% inhibition | Pseudomonas sp. | |
EDTA | 0.1 mM concentration 42% inhibition | Pseudomonas sp. | |
HgCl2 | 0.1 mM 36% inhibition | Pseudomonas sp. | |
hydroxylamine | 0.1 mM concentration 100% inhibition | Pseudomonas sp. | |
iodacetamide | 0.1 mM concentration 62% inhibition | Pseudomonas sp. | |
KBr | 1 mM concentration 58% inhibition | Pseudomonas sp. | |
KI | 1 mM concentration 94% inhibition | Pseudomonas sp. | |
o-phenanthroline | 0.1 mM concentration 26% inhibition | Pseudomonas sp. | |
Semicarbazide | 0.1 mM concentration 57% inhibition | Pseudomonas sp. | |
ZnSO4 | 0.1 mM concentration 12% inhibition | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.5 | - |
oxalic acid | - |
Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 0.09 atoms per subunit | Pseudomonas sp. | |
Mn2+ | 1.12 atoms per subunit | Pseudomonas sp. | |
Zn2+ | 0.36 atoms per subunit | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
alpha8, 8 * 38000, SDS-PAGE | Pseudomonas sp. |
320000 | - |
gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. OX-53 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
630 | - |
O2 consumption | Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-20°C, potassium phosphate buffer pH 6.5, 6 months, no activity loss | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-malic acid + O2 | - |
Pseudomonas sp. | ? | - |
? | |
DL-malic acid + O2 | - |
Pseudomonas sp. OX-53 | ? | - |
? | |
glyoxylic acid + O2 + 2 H+ | - |
Pseudomonas sp. | CO2 + formaldehyde + H2O2 | - |
? | |
glyoxylic acid + O2 + 2 H+ | - |
Pseudomonas sp. OX-53 | CO2 + formaldehyde + H2O2 | - |
? | |
maleic acid + O2 | - |
Pseudomonas sp. | ? | - |
? | |
maleic acid + O2 | - |
Pseudomonas sp. OX-53 | ? | - |
? | |
oxalic acid + O2 + 2 H+ | - |
Pseudomonas sp. | 2 CO2 + H2O2 | - |
ir | |
oxalic acid + O2 + 2 H+ | - |
Pseudomonas sp. OX-53 | 2 CO2 + H2O2 | - |
ir |
Subunits | Comment | Organism |
---|---|---|
octamer | alpha8, 8 * 38000, SDS-PAGE | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
after 10 min 100% activity | Pseudomonas sp. |
60 | - |
after 10 min, 76% activity | Pseudomonas sp. |
70 | - |
after 10 min 9% activity | Pseudomonas sp. |
89 | - |
after 3 min 49% activity | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
- |
Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7 | - |
Pseudomonas sp. |