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Literature summary for 1.2.3.4 extracted from

  • Moomaw, E.W.; Hoffer, E.; Moussatche, P.; Salerno, J.C.; Grant, M.; Immelman, B.; Uberto, R.; Ozarowski, A.; Angerhofer, A.
    Kinetic and spectroscopic studies of bicupin oxalate oxidase and putative active site mutants (2013), PLoS ONE, 8, e57933.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzymes are expressed in Escherichia coli BL21(DE3) cells Gelatoporia subvermispora

Protein Variants

Protein Variants Comment Organism
A242E the mutant shows 5% of the wild type oxidase activity Gelatoporia subvermispora
D241A the mutant contains 17% of the Mn2+ content of the recombinant wild type enzyme and 7% of the wild type oxidase activity Gelatoporia subvermispora
D241S inactive Gelatoporia subvermispora
R169K inactive Gelatoporia subvermispora
R349K inactive Gelatoporia subvermispora

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
oxalate wild type enzyme, pH and temperature not specified in the publication Gelatoporia subvermispora
1.1
-
oxalate mutant enzyme D241A, pH and temperature not specified in the publication Gelatoporia subvermispora
2
-
oxalate mutant enzyme A242E, pH and temperature not specified in the publication Gelatoporia subvermispora

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ dependent on Gelatoporia subvermispora
additional information not activated by Mg2+, Co2+, Zn2+, Ni2+, and Fe2+ Gelatoporia subvermispora

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oxalate + O2 + 2 H+ Gelatoporia subvermispora
-
CO2 + 2 H2O2
-
?

Organism

Organism UniProt Comment Textmining
Gelatoporia subvermispora
-
-
-

Purification (Commentary)

Purification (Comment) Organism
anion exchange column chromatography and butyl Sepharose column chromatography Gelatoporia subvermispora

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxalate + O2 + 2 H+
-
Gelatoporia subvermispora CO2 + 2 H2O2
-
?

Synonyms

Synonyms Comment Organism
OxOx
-
Gelatoporia subvermispora