Cloned (Comment) | Organism |
---|---|
His-tagged alpha and beta subunits and alpha2,beta2 tetramer, expression in Escherichia coli | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphorylation | - |
Bos taurus | |
phosphorylation | phosphorylation inactivates recombinant alpha2,beta2 tetramer in about 10 min | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
160000 | - |
recombinant alpha2,beta2 tetramer | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | - |
Bos taurus |
side-chain modification | phosphorylation of recombinant alpha2,beta2 tetramer, 3.25 phosphoryl groups per mol tetramer are incorporated, 50% phosphorylation within 10 min, only the alpha subunit is phosphorylated | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant alpha and beta subunits and recombinant alpha2,beta2 tetramer | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2 | two-step reaction: thiamine diphosphate dependent decarboxylation of pyruvate to 2-hydroxyethylidene-thiamine diphosphate and subsequent reductive acetylation of lipoic acid residues bound to the dihydrolipoamide acetyltransferase, EC 2.3.1.12 | Homo sapiens | |
pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2 | two-step reaction: thiamine diphosphate dependent decarboxylation of pyruvate to 2-hydroxyethylidene-thiamine diphosphate and subsequent reductive acetylation of lipoic acid residues bound to the dihydrolipoamide acetyltransferase, EC 2.3.1.12 | Bos taurus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0162 | - |
recombinant His-tagged-alpha2,beta2 tetramer, assay with 2-hydroxyethylidene-thiamine diphosphate and 2,6-dichlorphenolindophenol | Homo sapiens |
0.0269 | - |
recombinant His-tagged-alpha2,beta2 tetramer, assay with pyruvate and 2,6-dichlorphenolindophenol | Homo sapiens |
0.063 | - |
decarboxylation in the absence of K3Fe(CN)6 as artificial electron acceptor | Bos taurus |
0.13 | - |
recombinant alpha2beta2 tetramer | Homo sapiens |
0.1416 | - |
decarboxylation in the presence of K3Fe(CN)6 as artificial electron acceptor | Bos taurus |
0.145 | - |
recombinant alpha2,His-tagged-beta2 tetramer, K3Fe(CN)6 as artificial electron acceptor | Homo sapiens |
0.18 | - |
recombinant alpha2,beta2 tetramer with six histidine residues attached to the N-terminus | Homo sapiens |
0.187 | - |
recombinant His-tagged-alpha2,beta2 tetramer, K3Fe(CN)6 as artificial electron acceptor | Homo sapiens |
0.367 | - |
recombinant alpha2,His-tagged-beta2 tetramer, assay with pyruvate and 2,6-dichlorphenolindophenol | Homo sapiens |
10.9 | - |
recombinant alpha2,His-tagged-beta2 tetramer, reduction of NAD+, overall reaction | Homo sapiens |
14.4 | - |
recombinant His-tagged-alpha2,beta2 tetramer, reduction of NAD+, overall reaction | Homo sapiens |
15.8 | - |
reduction of NAD+, overall reaction | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxyethylidene-thiamine diphosphate + 2,6-dichlorophenolindophenol | - |
Homo sapiens | S-acetyldihydrolipoamide + reduced 2,6-dichlorophenolindophenol | - |
? | |
pyruvate + CoA + oxidized 2,6-dichlorophenolindophenol | - |
Homo sapiens | acetyl-CoA + CO2 + reduced 2,6-dichlorophenolindophenol | - |
? |