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Literature summary for 1.2.4.1 extracted from

  • Korotchkina, L.G.; Tucker, M.M.; Thekkumkara, T.J.; Madhusudhan, K.T.; Pons, G.; Kim, H.; Patel, M.S.
    Overexpression and characterization of human tetrameric pyruvate dehydrogenase and its individual subunits (1995), Protein Expr. Purif., 6, 79-90.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged alpha and beta subunits and alpha2,beta2 tetramer, expression in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
phosphorylation
-
Bos taurus
phosphorylation phosphorylation inactivates recombinant alpha2,beta2 tetramer in about 10 min Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
recombinant alpha2,beta2 tetramer Homo sapiens

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification
-
Bos taurus
side-chain modification phosphorylation of recombinant alpha2,beta2 tetramer, 3.25 phosphoryl groups per mol tetramer are incorporated, 50% phosphorylation within 10 min, only the alpha subunit is phosphorylated Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant alpha and beta subunits and recombinant alpha2,beta2 tetramer Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2 two-step reaction: thiamine diphosphate dependent decarboxylation of pyruvate to 2-hydroxyethylidene-thiamine diphosphate and subsequent reductive acetylation of lipoic acid residues bound to the dihydrolipoamide acetyltransferase, EC 2.3.1.12 Homo sapiens
pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2 two-step reaction: thiamine diphosphate dependent decarboxylation of pyruvate to 2-hydroxyethylidene-thiamine diphosphate and subsequent reductive acetylation of lipoic acid residues bound to the dihydrolipoamide acetyltransferase, EC 2.3.1.12 Bos taurus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0162
-
recombinant His-tagged-alpha2,beta2 tetramer, assay with 2-hydroxyethylidene-thiamine diphosphate and 2,6-dichlorphenolindophenol Homo sapiens
0.0269
-
recombinant His-tagged-alpha2,beta2 tetramer, assay with pyruvate and 2,6-dichlorphenolindophenol Homo sapiens
0.063
-
decarboxylation in the absence of K3Fe(CN)6 as artificial electron acceptor Bos taurus
0.13
-
recombinant alpha2beta2 tetramer Homo sapiens
0.1416
-
decarboxylation in the presence of K3Fe(CN)6 as artificial electron acceptor Bos taurus
0.145
-
recombinant alpha2,His-tagged-beta2 tetramer, K3Fe(CN)6 as artificial electron acceptor Homo sapiens
0.18
-
recombinant alpha2,beta2 tetramer with six histidine residues attached to the N-terminus Homo sapiens
0.187
-
recombinant His-tagged-alpha2,beta2 tetramer, K3Fe(CN)6 as artificial electron acceptor Homo sapiens
0.367
-
recombinant alpha2,His-tagged-beta2 tetramer, assay with pyruvate and 2,6-dichlorphenolindophenol Homo sapiens
10.9
-
recombinant alpha2,His-tagged-beta2 tetramer, reduction of NAD+, overall reaction Homo sapiens
14.4
-
recombinant His-tagged-alpha2,beta2 tetramer, reduction of NAD+, overall reaction Homo sapiens
15.8
-
reduction of NAD+, overall reaction Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxyethylidene-thiamine diphosphate + 2,6-dichlorophenolindophenol
-
Homo sapiens S-acetyldihydrolipoamide + reduced 2,6-dichlorophenolindophenol
-
?
pyruvate + CoA + oxidized 2,6-dichlorophenolindophenol
-
Homo sapiens acetyl-CoA + CO2 + reduced 2,6-dichlorophenolindophenol
-
?