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Literature summary for 1.2.4.2 extracted from

  • Green, S.R.; Storey, K.B.
    Regulation of the alpha-ketoglutarate dehydrogenasecomplex during hibernation in a small mammal, the Richardsons ground squirrel (Urocitellus richardsonii) (2020), Biochim. Biophys. Acta Proteins Proteom., 1868, 140448 .
    View publication on PubMed
Search for more literature for 1.2.4.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.29
-
 2-oxoglutarate overall reaction, enzyme from euthermic animals, 22°C, pH 7.8 Urocitellus richardsonii
0.29
-
 2-oxoglutarate overall reaction, enzyme from hibernating animals, 37°C, pH 7.8 Urocitellus richardsonii
0.32
-
 2-oxoglutarate overall reaction, enzyme from euthermic animals, 37°C, pH 7.8 Urocitellus richardsonii
0.38
-
 2-oxoglutarate overall reaction, enzyme from hibernating animals, 22°C, pH 7.8 Urocitellus richardsonii
0.4
-
 2-oxoglutarate overall reaction, enzyme from hibernating animals, 5°C, pH 7.8 Urocitellus richardsonii
0.45
-
 2-oxoglutarate overall reaction, enzyme from euthermic animals, 5°C, pH 7.8 Urocitellus richardsonii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 5 mM used in assay conditions Urocitellus richardsonii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine Urocitellus richardsonii
-
 [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Urocitellus richardsonii
-
-
-
Urocitellus richardsonii
-
 Richardsons ground squirrel
-

Purification (Commentary)

Purification (Comment) Organism
partial purification by 2% and 6% PEG (w/v) precipitations and Sephacryl S-400 gel filtration Urocitellus richardsonii
partially purified from skeletal muscle of euthermic and hibernating ground squirrels Urocitellus richardsonii

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle
-
 Urocitellus richardsonii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
252
-
 pH 7.8, 22°C, overall reaction of the complex Urocitellus richardsonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine
-
 Urocitellus richardsonii [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 116000, SDS-PAGE Urocitellus richardsonii

Synonyms

Synonyms Comment Organism
OGDH E1 subunit of the alpha-ketoglutarate dehydrogenase complex Urocitellus richardsonii

General Information

General Information Comment Organism
physiological function enzymes purified from skeletal muscle of euthermic and hibernating ground squirrels are evaluated at 5°C, 22°C, and 37 °C. The enzyme complex from hibernator muscle at all temperatures compared with euthermic controls exhibits a decreased affinity for CoA as well as reduced activation by Ca2+ ions at 5°C from both euthermic and hibernating conditions. The E1, E2 and E3 enzymes of the complex (OGDH, DLST, DLD) all show elevated phosphotyrosine content during hibernation as well as increased ADP-ribosylation and succinylation of hibernator OGDH Urocitellus richardsonii