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Literature summary for 1.2.4.2 extracted from
- Functional expression of a bacterial alpha-ketoglutarate dehydrogenase in the cytosol of Saccharomyces cerevisiae (2019), Metab. Eng., 56, 190-197 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | expression of the structural genes for all three subunits of the Escherichia coli 2-oxoglutarate dehydrogenase complex in Saccharomyces cerevisiae. The Escherichia coli lipoic-acid scavenging enzyme is coexpressed to enable cytosolic lipoylation of the complex, which is required for its enzymatic activity. In vivo cytosolic activity of the complex is tested by constructing a reporter strain in which the essential metabolite 5-aminolevulinic acid can only be synthetized from cytosolic, and not mitochondrial, succinyl-CoA. In the resulting strain, complementation of 5-aminolevulinic acid auxotrophy depends on activation of the 2-oxoglutarate dehydrogenase complex by lipoic acid addition | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Saccharomyces cerevisiae cytosol | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 1 mM used in assay conditions | Escherichia coli |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine | Escherichia coli | - |
[dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0AFG3 | - |
- |
| Escherichia coli | P0AFG3 | i.e. SucA, subunit E1 of the 2-oxoglutarate dehydrogenase complex | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Sephacryl S-500 gel filtration | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine | - |
Escherichia coli | [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-ketoglutarate dehydrogenase | - |
Escherichia coli |
| alphaKGDH | - |
Escherichia coli |
| SucA | - |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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