Cloned (Comment) | Organism |
---|---|
recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3), coexpression with superoxide dismutase of Thermus thermophilus in Escherichia coli, interaction analysis of 2-oxoisovalerate dehydrogenase alpha subunit with superoxid dismutase of Thermus thermophilus by yeast two hybrid system, recombinant expression in Saccharomyces cerevisiae | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | thermodynamics | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Thermus thermophilus | the 2-oxoisovalerate dehydrogenase alpha subunit interacts directly with the superoxid dismutase of Thermus thermophilus | ? | - |
? | |
additional information | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | the 2-oxoisovalerate dehydrogenase alpha subunit interacts directly with the superoxid dismutase of Thermus thermophilus | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q72GU1 | - |
- |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | Q72GU1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged enzyme from Escherichia coli strain Bl21(DE3) by glutathione affinity chromatography | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the 2-oxoisovalerate dehydrogenase alpha subunit interacts directly with the superoxid dismutase of Thermus thermophilus | Thermus thermophilus | ? | - |
? | |
additional information | the 2-oxoisovalerate dehydrogenase alpha subunit interacts directly with the superoxid dismutase of Thermus thermophilus | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2-oxoisovalerate dehydrogenase | - |
Thermus thermophilus |
branched-chain 2-oxo acid dehydrogenase | - |
Thermus thermophilus |
DHalpha | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Thermus thermophilus |
General Information | Comment | Organism |
---|---|---|
metabolism | the branched-chain alpha-ketoacid dehydrogenase complex (BCKDC) is a multisubunit complex of enzymes which catalyzes the oxidative decarboxylation of branched short chain alpha-ketoacids and function in the TCA cycle as key enzymes. Among them, the branched-chain 2-oxo acid dehydrogenase, 2-oxoisovalerate dehydrogenase, plays an essential role in the TCA cycle | Thermus thermophilus |