Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.2.5.2 extracted from

  • Gomez-Manzo, S.; Chavez-Pacheco, J.L.; Contreras-Zentella, M.; Sosa-Torres, M.E.; Arreguin-Espinosa, R.; Perez de la Mora, M.; Membrillo-Hernandez, J.; Escamilla, J.E.
    Molecular and catalytic properties of the aldehyde dehydrogenase of Gluconacetobacter diazotrophicus, a quinoheme protein containing pyrroloquinoline quinone, cytochrome b, and cytochrome c (2010), J. Bacteriol., 192, 5718-5724.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.2.5.2

Inhibitors

Inhibitors Comment Organism Structure
CuSO4 1 mM, cosubstrate ferricyanide, strong inhibition; 1 mM, strong inhibition Gluconacetobacter diazotrophicus
HgCl2 1 mM, cosubstrate ferricyanide, strong inhibition; 1 mM, strong inhibition Gluconacetobacter diazotrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3
-
 acetic acid cosubstrate ferricyandie, pH 3.5, 30°C Gluconacetobacter diazotrophicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane membrane-bound complex Gluconacetobacter diazotrophicus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 1 * 79700 + 1 * 50000, SDS-PAGE under denaturing conditions Gluconacetobacter diazotrophicus
79700
-
 1 * 79700 + 1 * 50000, SDS-PAGE under denaturing conditions Gluconacetobacter diazotrophicus
79700
-
 1 * 79700 and 1 * 50000,SDS-PAGE Gluconacetobacter diazotrophicus
129000
-
 PAGE Gluconacetobacter diazotrophicus
129000
-
 nondenaturing PAGE Gluconacetobacter diazotrophicus

Organism

Organism UniProt Comment Textmining
Gluconacetobacter diazotrophicus
-
-
-
Gluconacetobacter diazotrophicus ATCC 49037
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gluconacetobacter diazotrophicus

Storage Stability

Storage Stability Organism
-70°C, stable for several months Gluconacetobacter diazotrophicus
-70°C, stable for weeks without appreciable loss of activity Gluconacetobacter diazotrophicus
4°C, stable for several weeks Gluconacetobacter diazotrophicus
4°C, stable for several weeks without appreciable loss of activity Gluconacetobacter diazotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + 2 ferricyanide + H2O
-
 Gluconacetobacter diazotrophicus acetate + 2 ferrocyanide + 2 H+
-
 ?
acetaldehyde + 2 ferricyanide + H2O
-
 Gluconacetobacter diazotrophicus ATCC 49037 acetate + 2 ferrocyanide + 2 H+
-
 ?
acetaldehyde + ferricyanide
-
 Gluconacetobacter diazotrophicus acetic acid + ferrocyanide + H+
-
 ?
acetaldehyde + ferricyanide
-
 Gluconacetobacter diazotrophicus ATCC 49037 acetic acid + ferrocyanide + H+
-
 ?
acetaldehyde + oxidized phenazine methosulfate
-
 Gluconacetobacter diazotrophicus acetic acid + reduced phenazine methosulfate
-
 ?
acetaldehyde + oxidized phenazine methosulfate
-
 Gluconacetobacter diazotrophicus ATCC 49037 acetic acid + reduced phenazine methosulfate
-
 ?
glutaraldehyde + 2 ferricyanide + H2O 26.4% compared to the activity with acetaldehyde Gluconacetobacter diazotrophicus glutarate + 2 ferrocyanide + 2 H+
-
 ?
glutaraldehyde + oxidized phenazine methosulfate 26.5% of the activity with acetaldehyde Gluconacetobacter diazotrophicus glutaric acid + reduced phenazine methosulfate
-
 ?
additional information formaldehyde and benzaldehyde are not oxidized at all. Aliphatic alcohols and sugar aldehydes, are not substrates. The enzyme is specific for aliphatic aldehydes (C2-C6) and is able to use ferricyanide or phenazine methosulfate plus 2,6-dichlorophenolindophenol as electron acceptors having optimal pH values of 3.5 and 7.0, respectively. Ubiquinone-1 is also able to accept electrons from the dithionite-reduced enzyme Gluconacetobacter diazotrophicus ?
-
 ?
additional information no substrates: formaldehyde, benzaldehyde, aliphatic alcohols, sugar aldehydes Gluconacetobacter diazotrophicus ?
-
 ?
additional information formaldehyde and benzaldehyde are not oxidized at all. Aliphatic alcohols and sugar aldehydes, are not substrates. The enzyme is specific for aliphatic aldehydes (C2-C6) and is able to use ferricyanide or phenazine methosulfate plus 2,6-dichlorophenolindophenol as electron acceptors having optimal pH values of 3.5 and 7.0, respectively. Ubiquinone-1 is also able to accept electrons from the dithionite-reduced enzyme Gluconacetobacter diazotrophicus ATCC 49037 ?
-
 ?
additional information no substrates: formaldehyde, benzaldehyde, aliphatic alcohols, sugar aldehydes Gluconacetobacter diazotrophicus ATCC 49037 ?
-
 ?
propanal + oxidized phenazine methosulfate 72.7% of the activity with acetaldehyde Gluconacetobacter diazotrophicus propanoic acid + reduced phenazine methosulfate
-
 ?
propionaldehyde + 2 ferricyanide + H2O 72.7% compared to the activity with acetaldehyde Gluconacetobacter diazotrophicus propionate + 2 ferrocyanide + 2 H+
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 79700 + 1 * 50000, SDS-PAGE under denaturing conditions Gluconacetobacter diazotrophicus
heterodimer 1 * 79700 and 1 * 50000,SDS-PAGE Gluconacetobacter diazotrophicus

Synonyms

Synonyms Comment Organism
aldehyde dehydrogenase (acceptor)
-
 Gluconacetobacter diazotrophicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
22000
-
 acetic acid cosubstrate ferricyandie, pH 3.5, 30°C Gluconacetobacter diazotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
 electron accceptor ferricyanide Gluconacetobacter diazotrophicus
3.5
-
 using ferricyanide as an electron acceptor, the enzyme shows an optimum pH of 3.5 that shifts to pH 7.0 when phenazine methosulfate plus 2,6-dichlorophenolindophenol are the electron acceptors Gluconacetobacter diazotrophicus
7
-
 electron acceptor phenazine methosulfate plus 2,6-dichlorophenolindophenol Gluconacetobacter diazotrophicus
7
-
 using ferricyanide as an electron acceptor, the enzyme shows an optimum pH of 3.5 that shifts to pH 7.0 when phenazine methosulfate plus 2,6-dichlorophenolindophenol are the electron acceptors Gluconacetobacter diazotrophicus

Cofactor

Cofactor Comment Organism Structure
cytochrome b
-
 Gluconacetobacter diazotrophicus
cytochrome b the enzyme complex contains one cytochrome b Gluconacetobacter diazotrophicus
cytochrome c the smaller subunit bears three cytochromes c Gluconacetobacter diazotrophicus
pyrroloquinoline quinone
-
 Gluconacetobacter diazotrophicus

pI Value

Organism Comment pI Value Maximum pI Value
Gluconacetobacter diazotrophicus isoelectric focusing
-
 5.2

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6720
-
 acetic acid cosubstrate ferricyandie, pH 3.5, 30°C Gluconacetobacter diazotrophicus