Cloned (Comment) | Organism |
---|---|
the gene encoding the delta-subunit is expressed in Escherichia coli | Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the enzyme contains at least two [4Fe-4S] clusters. The purified and reconstituted delta subunit contains 8 Fe mol/mol | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + CoA + 2 oxidized ferredoxin | Pyrococcus furiosus | the enzyme catalyzes the final oxidative step in carbohydrate fermentation in which pyruvate is oxidized to acetyl-CoA and CO2, coupled to the reduction of ferredoxin | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinantly expressed delta subunit is purified after reconstitution with iron and sulfide. The reconstituted delta-subunit is monomeric with a mass of 11879 Da as determined by mass spectrometry. The purified and reconstituted delta subunit contains 8 Fe mol/mol and remains intact when incubated at 85 °C for 2 h | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate + CoA + 2 oxidized ferredoxin | the enzyme catalyzes the final oxidative step in carbohydrate fermentation in which pyruvate is oxidized to acetyl-CoA and CO2, coupled to the reduction of ferredoxin | Pyrococcus furiosus | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme is composed of two catalytic units of molecular mass 120000 Da. Each unit consists of four subunits, alpha/beta/gamma/delta, with masses of approximately 44000 Da, 36000 Da, 20000 Da, and 12000 Da, respectively, and contains at least two [4Fe-4S] clusters. The recombinantly expressed and reconstituted delta subunit is monomeric with a mass of 11879 Da | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
POR | - |
Pyrococcus furiosus |
pyruvate ferredoxin oxidoreductase | - |
Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
the recombinantly expressed, purified and reconstituted delta subunit is stable for 2 h | Pyrococcus furiosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
[4Fe-4S]-center | the enzyme contains at least two [4Fe-4S] clusters. The purified and reconstituted delta subunit contains 8 Fe mol/mol | Pyrococcus furiosus |