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Literature summary for 1.2.7.1 extracted from
- The pyruvate ferredoxin oxidoreductase of the thermophilic acetogen, Thermoanaerobacter kivui (2021), FEBS open bio, 11, 1332-1342 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | homologous protein production in Thermoacetobacter kivui by cloning and expression in Thermoacetobacter kivui. The encoded protein containing a genetically engineered His-tag is purified in only two steps to apparent homogeneity, and has the same properties as the native | Thermoanaerobacter kivui |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.019 | - |
oxidized ferredoxin | pH 7.5, 66°C | Thermoanaerobacter kivui |  |
| 0.025 | - |
CoA | pH 7.5, 66°C | Thermoanaerobacter kivui | |
| 0.11 | - |
pyruvate | pH 7.5, 66°C | Thermoanaerobacter kivui | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | 13.6 mol of iron per mol of protein | Thermoanaerobacter kivui | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 245000 | - |
gel filtration | Thermoanaerobacter kivui |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoanaerobacter kivui | A0A097APA2 | isoform Pfor1, NifJ1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification from cells grown on glucose | Thermoanaerobacter kivui |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.8 | - |
acceptor methylene blue, pH 7.5, 66°C | Thermoanaerobacter kivui |
| 27.2 | - |
acceptor ferredoxin, pH 7.5, 66°C | Thermoanaerobacter kivui |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 172 days, 70% residual activity | Thermoanaerobacter kivui |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate + CoA + 2 oxidized ferredoxin | - |
Thermoanaerobacter kivui | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
| pyruvate + CoA + oxidized methylene blue | - |
Thermoanaerobacter kivui | acetyl-CoA + CO2 + reduced methylene blue + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 130000, SDS-PAGE | Thermoanaerobacter kivui |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NifJ1 | - |
Thermoanaerobacter kivui |
| Pfor1 | - |
Thermoanaerobacter kivui |
| TKV_c04340 | - |
Thermoanaerobacter kivui |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 66 | - |
- |
Thermoanaerobacter kivui |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Thermoanaerobacter kivui |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
20% residual activity | Thermoanaerobacter kivui |
| 8 | - |
20% residual activity | Thermoanaerobacter kivui |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| coenzyme A | - |
Thermoanaerobacter kivui | |
| thiamine diphosphate | - |
Thermoanaerobacter kivui | |
| [4Fe-4S]-center | presence of three [4Fe-4S] clusters | Thermoanaerobacter kivui | |
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Release 2023.1 (January 2023)
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