KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.163 | - |
2-oxoglutarate | pH 7.0, 55°C | Saccharolobus solfataricus | |
0.275 | - |
pyruvate | pH 7.0, 55°C | Saccharolobus solfataricus | |
0.516 | - |
2-oxobutyrate | pH 7.0, 55°C | Saccharolobus solfataricus | |
0.516 | - |
2-oxobutanoate | pH 7.0, 55°C | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
1 * 69000 (alpha-subunit) + 1 * 34000 (beta-subunit), SDS-PAGE, MALDI-TOF mass spectrometry | Saccharolobus solfataricus |
34000 | - |
1 * 69000, alpha-subuit, + 1 * 34000, beta-subunit, SDS-PAGE and MALDI-TOF | Saccharolobus solfataricus |
69000 | - |
1 * 69000 (alpha-subunit) + 1 * 34000 (beta-subunit), SDS-PAGE, MALDI-TOF mass spectrometry | Saccharolobus solfataricus |
69000 | - |
1 * 69000, alpha-subuit, + 1 * 34000, beta-subunit, SDS-PAGE and MALDI-TOF | Saccharolobus solfataricus |
103000 | - |
native PAGE | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus DSM 1616 | - |
- |
- |
Saccharolobus solfataricus P1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
52.6 | - |
pH 7.0, 55°C | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutanoate + CoA + 2 oxidized cytochrome c | - |
Saccharolobus solfataricus | propanoyl-CoA + CO2 + 2 reduced cytochrome c + 2 H+ | - |
? | |
2-oxobutyrate + CoA + oxidized ferredoxin | Sulfolobus solfataricus ferredoxin contains a dicluster, i.e., a [3Fe-4S] cluster and a [4Fe-4S] cluster, and to possibly contain one zinc center | Saccharolobus solfataricus | propanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
2-oxobutyrate + CoA + oxidized ferredoxin | Sulfolobus solfataricus ferredoxin contains a dicluster, i.e., a [3Fe-4S] cluster and a [4Fe-4S] cluster, and to possibly contain one zinc center | Saccharolobus solfataricus P1 | propanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
2-oxobutyrate + CoA + oxidized ferredoxin | Sulfolobus solfataricus ferredoxin contains a dicluster, i.e., a [3Fe-4S] cluster and a [4Fe-4S] cluster, and to possibly contain one zinc center | Saccharolobus solfataricus DSM 1616 | propanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
2-oxoglutarate + CoA + 2 oxidized cytochrome c | best substrate tested | Saccharolobus solfataricus | succinyl-CoA + CO2 + 2 reduced cytochrome c + 2 H+ | - |
? | |
2-oxoglutarate + CoA + 2 oxidized ferredoxin | relative activity is 20.4% compared to 2-oxoglutarate, kcat/Km for 2-oxoglutarate is 6.5% compared to kcat/Km for 2-oxobutyrate | Saccharolobus solfataricus | succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
2-oxoglutarate + CoA + 2 oxidized ferredoxin | relative activity is 20.4% compared to 2-oxoglutarate, kcat/Km for 2-oxoglutarate is 6.5% compared to kcat/Km for 2-oxobutyrate | Saccharolobus solfataricus P1 | succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
2-oxoglutarate + CoA + 2 oxidized ferredoxin | relative activity is 20.4% compared to 2-oxoglutarate, kcat/Km for 2-oxoglutarate is 6.5% compared to kcat/Km for 2-oxobutyrate | Saccharolobus solfataricus DSM 1616 | succinyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
additional information | no activity with glyoxylate | Saccharolobus solfataricus | ? | - |
? | |
additional information | enzyme displays broad substrate specificity toward 2-oxoacids, such as pyruvate, 2-oxobutanoate, and 2-oxoglutarate | Saccharolobus solfataricus | ? | - |
? | |
additional information | no activity with glyoxylate | Saccharolobus solfataricus P1 | ? | - |
? | |
additional information | enzyme displays broad substrate specificity toward 2-oxoacids, such as pyruvate, 2-oxobutanoate, and 2-oxoglutarate | Saccharolobus solfataricus P1 | ? | - |
? | |
additional information | no activity with glyoxylate | Saccharolobus solfataricus DSM 1616 | ? | - |
? | |
additional information | enzyme displays broad substrate specificity toward 2-oxoacids, such as pyruvate, 2-oxobutanoate, and 2-oxoglutarate | Saccharolobus solfataricus DSM 1616 | ? | - |
? | |
pyruvate + CoA + 2 oxidized cytochrome c | - |
Saccharolobus solfataricus | acetyl-CoA + CO2 + 2 reduced cytochrome c + 2 H+ | - |
? | |
pyruvate + CoA + 2 oxidized ferredoxin | relative activity is 31.2% compared to 2-oxoglutarate, kcat/Km for pyruvate is 19% compared to kcat/Km for 2-oxobutyrate | Saccharolobus solfataricus | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
pyruvate + CoA + 2 oxidized ferredoxin | relative activity is 31.2% compared to 2-oxoglutarate, kcat/Km for pyruvate is 19% compared to kcat/Km for 2-oxobutyrate | Saccharolobus solfataricus P1 | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? | |
pyruvate + CoA + 2 oxidized ferredoxin | relative activity is 31.2% compared to 2-oxoglutarate, kcat/Km for pyruvate is 19% compared to kcat/Km for 2-oxobutyrate | Saccharolobus solfataricus DSM 1616 | acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 69000, alpha-subuit, + 1 * 34000, beta-subunit, SDS-PAGE and MALDI-TOF | Saccharolobus solfataricus |
heterodimer | 1 * 69000 (alpha-subunit) + 1 * 34000 (beta-subunit), SDS-PAGE, MALDI-TOF mass spectrometry | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
assay at | Saccharolobus solfataricus |
70 | - |
- |
Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
2-oxobutanoate | pH 7.0, 55°C | Saccharolobus solfataricus | |
1.55 | - |
2-oxobutyrate | pH 7.0, 55°C | Saccharolobus solfataricus | |
2.4 | - |
pyruvate | pH 7.0, 55°C | Saccharolobus solfataricus | |
7.5 | - |
2-oxoglutarate | pH 7.0, 55°C | Saccharolobus solfataricus | |
7.53 | - |
2-oxoglutarate | pH 7.0, 55°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Saccharolobus solfataricus |
7 | - |
assay at | Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6.0: about 50% of maximal activity, pH 9.0: about 50% of maximal activity | Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | enzyme contains a dicluster, [3Fe-4S][4Fe-4S], type ferredoxin of 11000 Da by SDS-PAGE and of 11180 Da by MALDI-TOF mass spectrometry | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
2-oxobutyrate | pH 7.0, 55°C | Saccharolobus solfataricus | |
3 | - |
2-oxobutanoate | pH 7.0, 55°C | Saccharolobus solfataricus | |
8.7 | - |
pyruvate | pH 7.0, 55°C | Saccharolobus solfataricus | |
8.73 | - |
pyruvate | pH 7.0, 55°C | Saccharolobus solfataricus | |
46 | - |
2-oxoglutarate | pH 7.0, 55°C | Saccharolobus solfataricus | |
46.2 | - |
2-oxoglutarate | pH 7.0, 55°C | Saccharolobus solfataricus |