Literature summary for 1.2.7.11 extracted from

Witt, A.; Pozzi, R.; Diesch, S.; Haedicke, O.; Grammel, H.
New light on ancient enzymes - in vitro CO2 fixation by pyruvate synthase of Desulfovibrio africanus and Sulfolobus acidocaldarius (2019), FEBS J., 286, 4494-4508 .

Search for more literature for 1.2.7.11

Application

Application	Comment	Organism
analysis	biochemical assay for activity, low-potential electrons are introduced by photochemical reduction of EDTA/deazaflavin and the generated pyruvate is trapped by chemical derivatization with semicarbazide. The product of CO2 fixation can be detected as pyruvate semicarbazone by HPLC-MS	Sulfolobus acidocaldarius

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Sulfolobus acidocaldarius

Organism

Organism	UniProt	Comment	Textmining
Sulfolobus acidocaldarius	Q4J6I9 and Q4J6I8	Q4J6I9 i.e. alpha subunit, Q4J6I8 i.e. beta subunit KorB	-
Sulfolobus acidocaldarius DSM 639	Q4J6I9 and Q4J6I8	Q4J6I9 i.e. alpha subunit, Q4J6I8 i.e. beta subunit KorB	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
10	-	acceptor methyl viologen, pH 9, 70°C	Sulfolobus acidocaldarius

Storage Stability

Storage Stability	Organism
4°C, Tris/HCl (20 mM), pH 8.5, 32 days, no loss of catalytic activity	Sulfolobus acidocaldarius

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-oxoglutarate + CoA + oxidized methyl viologen	-	Sulfolobus acidocaldarius	succinoyl-CoA + CO2 + reduced methyl viologen	-	r
2-oxoglutarate + CoA + oxidized methyl viologen	-	Sulfolobus acidocaldarius DSM 639	succinoyl-CoA + CO2 + reduced methyl viologen	-	r
acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+	-	Sulfolobus acidocaldarius	pyruvate + CoA + 2 oxidized ferredoxin	-	r
acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+	-	Sulfolobus acidocaldarius DSM 639	pyruvate + CoA + 2 oxidized ferredoxin	-	r
acetyl-CoA + CO2 + 2 reduced methyl viologen + 2 H+	-	Sulfolobus acidocaldarius	pyruvate + CoA + 2 oxidized methyl viologen	-	r
additional information	enzyme is able to use low-potential ferredoxins of the green sulfur bacterium Chlorobium tepidum and of Sulfolobus acidocaldarius whereas CO2 fixation is not supported by the native ferredoxin of Desulfocurvibacter africanus. Methyl viologen as an artificial electron carrier also allows CO2 fixation	Sulfolobus acidocaldarius	?	-	-
additional information	enzyme is able to use low-potential ferredoxins of the green sulfur bacterium Chlorobium tepidum and of Sulfolobus acidocaldarius whereas CO2 fixation is not supported by the native ferredoxin of Desulfocurvibacter africanus. Methyl viologen as an artificial electron carrier also allows CO2 fixation	Sulfolobus acidocaldarius DSM 639	?	-	-
pyruvate + CoA + 2 oxidized ferredoxin	-	Sulfolobus acidocaldarius	acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+	-	r
pyruvate + CoA + 2 oxidized ferredoxin	-	Sulfolobus acidocaldarius DSM 639	acetyl-CoA + CO2 + 2 reduced ferredoxin + 2 H+	-	r
pyruvate + CoA + 2 oxidized methyl viologen	-	Sulfolobus acidocaldarius	acetyl-CoA + CO2 + 2 reduced methyl viologen + 2 H+	-	r
pyruvate + CoA + 2 oxidized methyl viologen	-	Sulfolobus acidocaldarius DSM 639	acetyl-CoA + CO2 + 2 reduced methyl viologen + 2 H+	-	r

Subunits

Subunits	Comment	Organism
?	x * 71000 plus x * 34000, SDS-PAGE	Sulfolobus acidocaldarius

Synonyms

Synonyms	Comment	Organism
PFOR	-	Sulfolobus acidocaldarius
Saci_2306	-	Sulfolobus acidocaldarius
Saci_2307	-	Sulfolobus acidocaldarius

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
70	-	-	Sulfolobus acidocaldarius

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
10	-	-	Sulfolobus acidocaldarius

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Release 2023.1 (January 2023)